Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,832,443 G→A 8.3% P130P (CCG→CCA)  PP_1635 → two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,832,4430GA 8.3% 134.4 / 2.7 48P130P (CCG→CCA) PP_1635two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (24/20);  new base A (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

GTGATGGTTTCGGCACAGGAAGAGGCCGCCGTGGTGGTCAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAACAAGCAGATCGCCTATGAGCTGAGCG  >  NC_002947/1832300‑1832588
                                                                                                                                               |                                                                                                                                                   
gtgATGGTTTCGGCACAGGAAGAGGCCGCCGTGGTGGTCAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAg                                                                                                                                               <  2:199388/150‑1 (MQ=255)
            gCACAGGAAGAGGCCGCCGTGGTGGTCAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAg                                                                                                                                   >  2:347024/1‑150 (MQ=255)
               caGGAAGAGGCCGCCGTGGTGGTCAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTc                                                                                                                                <  2:143870/150‑1 (MQ=255)
                  gAAGAGGCCGCCGTGGTGGTCAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCCGGCGTTCGAAAAGTTCGAt                                                                                                                             <  2:1043545/150‑1 (MQ=255)
                        gccgccGTGGTGGTCAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGAt                                                                                                                             >  2:460144/1‑144 (MQ=255)
                        gccgccGTGGTGGTCAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGAt                                                                                                                             <  1:460144/144‑1 (MQ=255)
                        gccgccGTGGTGGTCAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCg                                                                                                                       >  1:877224/1‑150 (MQ=255)
                                    gtCAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAg                                                                                                           <  1:347024/150‑1 (MQ=255)
                                      cAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGgc                                                                                                         <  1:26461/150‑1 (MQ=255)
                                            ccGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAg                                                                                                   >  1:1222343/1‑150 (MQ=255)
                                             cgcgAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGttt                                                                                                                         <  1:102225/127‑1 (MQ=255)
                                             cgcgAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGttt                                                                                                                         >  2:102225/1‑127 (MQ=255)
                                              gcgAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCg                                                                                                 >  2:732219/1‑150 (MQ=255)
                                                gAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCgcc                                                                                               <  1:1009758/150‑3 (MQ=255)
                                                       gcgcCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTg‑‑g                                                                                      <  1:262543/150‑1 (MQ=255)
                                                                gTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCa                                                                             >  2:770831/1‑150 (MQ=255)
                                                                gTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCa                                                                             >  2:775449/1‑150 (MQ=255)
                                                                 tttCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCAc                                                                            <  2:1222343/150‑1 (MQ=255)
                                                                  ttCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCAcg                                                                           >  2:772863/1‑150 (MQ=255)
                                                                   tCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGATAGGCAGTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCAcgc                                                                          >  1:78856/1‑150 (MQ=255)
                                                                      ttCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCCACGGCGCGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCATCAcgccgc                                                                       >  2:632885/1‑150 (MQ=255)
                                                                         ccAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCgcagc                                                                    >  1:1084099/1‑150 (MQ=255)
                                                                         ccAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCgcagc                                                                    >  2:188105/1‑150 (MQ=255)
                                                                           aaGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTg‑‑g                                                                                      <  1:265286/130‑1 (MQ=255)
                                                                           aaGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTg‑‑g                                                                                      >  2:265286/1‑130 (MQ=255)
                                                                                cAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCcg                                                             >  1:1056000/1‑150 (MQ=255)
                                                                                 aGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCAcgc                                                                          >  2:1072721/1‑136 (MQ=255)
                                                                                 aGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCAcgc                                                                          <  1:1072721/136‑1 (MQ=255)
                                                                                      ccTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACt                                                       >  2:1078400/1‑150 (MQ=255)
                                                                                       cTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTg                                                      >  2:348651/1‑150 (MQ=255)
                                                                                             gTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATg                                                <  1:770831/150‑1 (MQ=255)
                                                                                                 tCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCACCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGaa                                                                                               <  2:174248/101‑1 (MQ=255)
                                                                                                 tCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCACCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGaa                                                                                               <  2:174289/101‑1 (MQ=255)
                                                                                                 tCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCACCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGaa                                                                                               >  1:174248/1‑101 (MQ=255)
                                                                                                 tCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCACCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGaa                                                                                               >  1:174289/1‑101 (MQ=255)
                                                                                                   cAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGc                                          <  1:772863/150‑1 (MQ=255)
                                                                                                   cAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTAGGACGCGGCCAAGGACGAC‑TCGAATG‑TTGCTGTCATCCGCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGc                                          <  1:348651/150‑1 (MQ=255)
                                                                                                           ggTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCtt                                  >  2:612838/1‑150 (MQ=255)
                                                                                                            gTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTg                                 >  2:807783/1‑150 (MQ=255)
                                                                                                                  aaGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGCAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAAc                           >  2:931250/1‑150 (MQ=255)
                                                                                                                                   ggTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAACAAGCAGATCGCCTAtga          >  2:4496/1‑150 (MQ=255)
                                                                                                                                       tggtggCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAACAAGCAGATCGCCTAtgagctg      >  1:962817/1‑150 (MQ=255)
                                                                                                                                        ggtggCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAACAAGCAGATCGCCTAtgagctga     <  1:775449/150‑1 (MQ=255)
                                                                                                                                          tggCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAACAAGCAGATCGCCTAtgagctgagc   <  2:877224/150‑1 (MQ=255)
                                                                                                                                          tggCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAACAAGCAGATCGCCTAtgagctgagc   <  2:962817/150‑1 (MQ=255)
                                                                                                                                          tggCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAACAAGCAGATCGCCTAtgagctgagc   <  1:612838/150‑1 (MQ=255)
                                                                                                                                          tggCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAACAAGCAGATCGCCTAtgagctgagc   <  1:807783/150‑1 (MQ=255)
                                                                                                                                           ggCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAACAAGCAGATCGCCTATGAGCTGAGCg  >  2:1072732/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GTGATGGTTTCGGCACAGGAAGAGGCCGCCGTGGTGGTCAAGTCCCGCGAGTTCGGCGCCAGTGGTTTCATTCCCAAGTCCAGCACCCTGGAAGTGATCCAGGATGCGGTGCGCAAGGTACTCGACGGCGAGGTCTGGTGGCCGCCGCAGGCGTTCGAAAAGGTCGATGTTTCGGCCGAGGCCAAGGCCGCCAGCGAAGGCTTG‑‑GCCAGCCTCACGCCGCAGCAGTTCCGCGTACTGACCATGGTCTGCGAAGGCTTGCTGAACAAGCAGATCGCCTATGAGCTGAGCG  >  NC_002947/1832300‑1832588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑