| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,955,253 | T→A | 12.8% | intergenic (+40/‑153) | PP_1752 → / → PP_1753 | hypothetical protein/DMT superfamily permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,955,253 | 0 | T | A | 12.8% | 129.9 / 11.5 | 49 | intergenic (+40/‑153) | PP_1752/PP_1753 | hypothetical protein/DMT superfamily permease |
| Reads supporting (aligned to +/- strand): ref base T (25/16); new base A (3/3); total (29/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01 | |||||||||||
TTGCCCCTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATCGAG > NC_002947/1955104‑1955399 | ttGCCCCTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGttt < 2:1093263/150‑1 (MQ=255) ccTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCcg > 2:606516/1‑150 (MQ=255) cTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCcgc > 2:939981/1‑150 (MQ=255) cTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCcgc > 2:515740/1‑150 (MQ=255) cTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGTTTCGCGGGTAAACCCGCGaagcggc < 2:765012/150‑6 (MQ=255) tggtggAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCGGCGAAAGGGTc > 2:1056417/1‑150 (MQ=255) gAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGcac > 1:1038659/1‑150 (MQ=255) aGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGcaca > 1:891263/1‑150 (MQ=255) ggCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAg > 1:245751/1‑150 (MQ=255) ggCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAg < 1:748123/150‑1 (MQ=255) ggCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAg < 1:515740/150‑1 (MQ=255) cAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCa > 1:165091/1‑150 (MQ=255) aTGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAac < 2:891263/150‑1 (MQ=255) ggtggtGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGaagc < 2:308406/109‑3 (MQ=255) ggtggtGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGaagc > 1:308406/1‑107 (MQ=255) ggtggtGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCaaaa < 1:639778/150‑1 (MQ=255) ggtggtGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGACGCCTCGCGGCTTCTCCCGCGAAAGGGTCATCACTGGCAACACCTCCACCCAGCCATGCCCCaaaa < 2:787933/150‑1 (MQ=255) gtgCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAAt > 1:832724/1‑150 (MQ=255) tCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAAt < 1:543338/140‑1 (MQ=255) tCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAAt > 2:543338/1‑140 (MQ=255) agcAGGCGCTTGAGGCGGCGCTTCCCACAGC‑CGAGCACTAGCAAGCGGTGGGGTTCGCTGTTCTAGTCGATTCGCTGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAAtt < 2:908366/148‑1 (MQ=255) aCCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAAtt < 2:165091/150‑1 (MQ=255) ccAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAg > 1:375014/1‑102 (MQ=255) ccAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAg < 2:375014/102‑1 (MQ=255) cAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCt > 1:110893/1‑150 (MQ=255) aGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCtt < 2:110893/150‑1 (MQ=255) ggCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGCATGACCTGATGACTTCttt > 1:1145950/1‑150 (MQ=255) ggCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGa < 1:1136804/135‑1 (MQ=255) ggCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGa > 2:1136804/1‑135 (MQ=255) cA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGtct > 2:364978/1‑150 (MQ=255) caAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGACCCTTTCGCGGGTAAACCCGCGaagc > 1:1072277/1‑59 (MQ=11) caAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGACCCTTTCGCGGGTAAACCCGCGaagc < 2:1072277/61‑3 (MQ=11) aTAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAAc > 1:1080411/1‑150 (MQ=255) ccGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCg > 2:411667/1‑150 (MQ=255) gAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTa < 2:1080411/150‑1 (MQ=255) ggTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTcaa < 2:245751/150‑1 (MQ=255) gTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTcaac > 1:583672/1‑150 (MQ=255) ttGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTcaaca > 2:1027890/1‑150 (MQ=255) ttGCCTGTGCTGACCCTTTCGCGGGTAAACCCGCGAAGCGGCCAGAACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTcaaca > 1:189120/1‑150 (MQ=21) cTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAAccc > 2:205725/1‑150 (MQ=255) tGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCccacca > 2:1281936/1‑150 (MQ=255) gaccctttCGCGGGTAAACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCcaccac > 2:748113/7‑150 (MQ=255) gCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACg > 2:662840/1‑150 (MQ=255) cGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACggg > 2:123366/1‑150 (MQ=255) tCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTg < 1:364978/150‑1 (MQ=255) tCGCGGGTAAACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTg < 1:748113/150‑1 (MQ=255) gcgcGTTTACCCGCGAAAGGGTCAGCACACGAAAAACATCAAGGCAGCCAGGCCCCAAAAGAATGACCTAATTTCTCCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGAt < 1:606516/150‑1 (MQ=255) cgGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATc > 1:58177/1‑150 (MQ=255) ggTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACAACGGGCTTGATCGa < 2:857308/150‑1 (MQ=255) gTTGACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATCGAg > 2:17706/1‑150 (MQ=255) | TTGCCCCTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCA‑AGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATCGAG > NC_002947/1955104‑1955399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |