| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,445,402 | G→C | 19.0% | A51A (GCC→GCG) | lexA‑I ← | transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,445,402 | 0 | G | C | 19.0% | 132.9 / 29.1 | 58 | A51A (GCC→GCG) | lexA‑I | transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (20/27); new base C (5/6); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-01 | |||||||||||
TGCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTTGGCGTGGCGTC > NC_002947/2445265‑2445543 | tgCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCCCGCAAGGGcgcga < 2:351517/150‑5 (MQ=255) gCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGa < 2:706800/150‑1 (MQ=255) gCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGa < 2:256753/150‑1 (MQ=255) cTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAg > 2:1058915/1‑150 (MQ=255) cTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAg < 1:700391/150‑1 (MQ=255) tGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTg > 1:640465/1‑147 (MQ=255) tGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTg < 2:640465/147‑1 (MQ=255) tGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAgg < 1:562656/150‑1 (MQ=255) ggCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGctc > 2:881897/1‑150 (MQ=255) ggCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGctc < 1:1058915/150‑1 (MQ=255) gCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGctcc > 1:184768/1‑150 (MQ=255) aaTCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGTATGCCGCGGTAGCCGGCTGGCTGGATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTcggc > 2:467706/1‑150 (MQ=255) aaTCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTcggc < 2:1124343/150‑1 (MQ=255) gcACCGGCAGCGTCTCGGCCGATGATGTGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTg > 2:258184/1‑150 (MQ=255) ccGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCATGCCAGGTACGCGCTGGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGc < 1:664604/150‑1 (MQ=255) ccGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGcc < 2:360735/150‑1 (MQ=255) ccGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCCCGCAAGGGc > 2:1129278/1‑111 (MQ=255) ccGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCCCGCAAGGGc < 1:1129278/111‑1 (MQ=255) atgGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCCCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGa < 2:950108/150‑1 (MQ=255) tgGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAg < 2:184768/150‑1 (MQ=255) ggCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAggg > 1:80042/1‑97 (MQ=255) ggCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAggg < 2:80042/97‑1 (MQ=255) gCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCATTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCaa > 1:22476/1‑150 (MQ=255) cAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAAt < 1:467706/150‑1 (MQ=255) cAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCgg > 1:57902/1‑146 (MQ=255) cAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCgg < 2:57902/146‑1 (MQ=255) cAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAg < 1:1037369/150‑1 (MQ=255) ttGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCggg > 2:632501/1‑150 (MQ=255) ttCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCGTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGcc > 1:473819/1‑150 (MQ=255) tCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCg > 1:233990/1‑150 (MQ=255) caggccaggGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTGGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGTAATCTCGGCGCGGGTAGTCGGGAAGCCGtt < 1:881897/150‑1 (MQ=255) caggccaggGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTTGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGtt > 1:210888/1‑150 (MQ=255) caggccaggGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGtt < 2:22476/150‑1 (MQ=255) caggccaggGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCATTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGtt < 1:258184/150‑1 (MQ=255) caggGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCgg > 1:953564/1‑136 (MQ=255) caggGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCgg < 2:953564/136‑1 (MQ=255) ggatgcCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCACACCGGGGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAgc < 1:80024/150‑1 (MQ=255) gatgcCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAAt < 2:144143/108‑1 (MQ=255) gatgcCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAAt > 1:144143/1‑108 (MQ=255) gcCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCgg < 1:604017/115‑1 (MQ=255) gcCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCgg > 2:604017/1‑115 (MQ=255) cgcgGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGCCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTtga < 1:632501/150‑1 (MQ=255) cgcCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCga < 2:1274330/150‑1 (MQ=255) aTTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCt > 1:1080950/1‑150 (MQ=255) tttCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCtt < 2:233990/150‑1 (MQ=255) aTCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAgc > 1:564719/1‑118 (MQ=255) aTCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAgc < 2:564719/118‑1 (MQ=255) aTCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTtggcgt > 2:962033/1‑150 (MQ=255) aaggcCCTTGCCCGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGcc < 2:1012539/96‑1 (MQ=255) aaggcCCTTGCCCGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGcc > 1:1012539/4‑99 (MQ=255) aaggcCCTTGCCCGCAAGGGCCTTGAGGCGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGtt > 2:486948/4‑102 (MQ=255) aaggcCCTTGCCCGCAAGGGCCTTGAGGCGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGtt < 1:486948/99‑1 (MQ=255) ggcCCTTGCCCGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCa > 2:755117/2‑108 (MQ=255) ggcCCTTGCCCGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCa < 1:755117/107‑1 (MQ=255) gcCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTTggcgtggcgt > 1:806415/1‑150 (MQ=255) gcCCTTGCCCGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCtt > 2:673159/1‑140 (MQ=255) gcCCTTGCCCGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCtt < 1:673159/140‑1 (MQ=255) cccTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTTGGCGTGGCGTc < 2:473819/150‑1 (MQ=255) | TGCTGTTCGGCAAGAATCGGCGCACCGGCAGCGACCCGGCCGATGATGGGCAGGCCGGCTTCTTCAGCCTTGGCTTCCAGGCCAGGGATGCGGATGCCGCGGGAAGCGCCCGGCGTCATTTCGATCGCGCCCTTGCGGGCAAGGGCCTTGAGGTGCTCCTCGGCGGCATTGGGCGACTTGAAGCCCAGCTCCTGAGCAATCTCGGCGCGGGTAGGCGGGAAGCCGTTGTCTTCCAGGCAGCGCTTGATGAACGCGAGAATTTCGGCTTGGCGTGGCGTC > NC_002947/2445265‑2445543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |