Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,462,973 T→A 13.7% L229Q (CTG→CAG)  lolD → lipoprotein‑releasing system ATP‑binding protein LolD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,462,9730TA13.7% 137.0 / 15.1 51L229Q (CTG→CAG) lolDlipoprotein‑releasing system ATP‑binding protein LolD
Reads supporting (aligned to +/- strand):  ref base T (23/21);  new base A (4/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01

CTCGACCACCATACCGCCCAAGGCATCCAGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGCCAAGCGTCGCAACCACTTC  >  NC_002947/2462831‑2463112
                                                                                                                                              |                                                                                                                                           
cTCGACCACCATACCGCCCAAGGCATCCAGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGcc                                                                                                                                      >  2:280582/1‑150 (MQ=255)
 tCGACCACCATACCGCCCAAGGCATCCAGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCa                                                                                                                                     >  2:1075263/1‑150 (MQ=255)
      caccaTACCGCCCAAGGCATCCAGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGa                                                                                                                                <  2:1113701/150‑1 (MQ=255)
      caccaTACCGCCCAAGGCATCCAGGAGCTGATGCAGGAGCGGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGa                                                                                                                                >  1:28547/1‑150 (MQ=255)
        ccaTACCGCCCAAGGCATCCAGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATc                                                                                                                              <  2:951720/150‑1 (MQ=255)
         caTACCGCCCAAGGCATCCAGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCg                                                                                                                             >  1:109371/1‑150 (MQ=255)
         caTACCGCCCAAGGCATCCAGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCAGGTGGCCATcgtcgag                                                                                                                             <  1:128556/150‑7 (MQ=255)
               gCCCAAGGCATCCAGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGcc                                                                                                                       >  1:1090779/1‑150 (MQ=255)
                           cAGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGt                                                                                                           >  1:587075/1‑150 (MQ=255)
                            aGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGtg                                                                                                          >  1:1193691/1‑150 (MQ=255)
                            aGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGtg                                                                                                          <  2:1021552/150‑1 (MQ=255)
                            aGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCAGGTGGCCATc                                                                                                                                   <  1:1152877/125‑1 (MQ=255)
                            aGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCAGGTGGCCATc                                                                                                                                   >  2:1152877/1‑125 (MQ=255)
                              gAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCAGGTGGCCATc                                                                                                                                   >  2:509481/1‑123 (MQ=255)
                              gAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCAGGTGGCCATc                                                                                                                                   <  1:509481/123‑1 (MQ=255)
                                        aGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGtgctgc                                                                                                      <  2:226541/142‑1 (MQ=255)
                                        aGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGtgctgc                                                                                                      >  1:226541/1‑142 (MQ=255)
                                         ggAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTc                                                                                             >  1:1177256/1‑150 (MQ=255)
                                                tCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTc                                                                                      >  2:164775/1‑150 (MQ=255)
                                                      gcTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTgg                                                                                <  2:109371/150‑1 (MQ=255)
                                                        ttCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGt                                                                              <  1:378846/150‑1 (MQ=255)
                                                           gcgcACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCCGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTggt                                                                           >  2:87835/1‑148 (MQ=255)
                                                               aCGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTgg                                                                                >  1:1019669/1‑141 (MQ=255)
                                                               aCGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTgg                                                                                <  2:1019669/141‑1 (MQ=255)
                                                                 ggCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTa                                                                     >  2:96685/1‑150 (MQ=255)
                                                                 ggCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTa                                                                     >  1:1016947/1‑150 (MQ=255)
                                                                    aTTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCNTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCGGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACAt                                                                  >  1:626381/1‑150 (MQ=255)
                                                                    aTTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCGATTGGGTTGTTTCGTACAt                                                                  >  2:874076/1‑150 (MQ=255)
                                                                      tCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTgg                                                                                >  1:424233/1‑134 (MQ=255)
                                                                      tCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTgg                                                                                <  2:424233/134‑1 (MQ=255)
                                                                                 aCCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCTCGGTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTg                                                     >  1:781842/1‑150 (MQ=255)
                                                                                   ccaccaCCTCAACCTGGCGCGGCACATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGcc                                                   <  2:626381/150‑1 (MQ=255)
                                                                                   ccACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGcc                                                   >  1:678604/1‑150 (MQ=255)
                                                                                    cACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGccc                                                  <  2:1193691/150‑1 (MQ=255)
                                                                                    cACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGccc                                                  <  2:587075/150‑1 (MQ=255)
                                                                                        aCCTCAACCTGGAGCGGCAGATGGACCGCGAGCTAAAGCTCTACGATGTCCACCTGGTTGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCt                                              <  2:781842/150‑1 (MQ=255)
                                                                                          cTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCttt                                            >  2:541687/1‑150 (MQ=255)
                                                                                            cAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTAt                                          <  1:164775/150‑1 (MQ=255)
                                                                                                         cAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTac                             <  1:280582/150‑1 (MQ=255)
                                                                                                             tGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACAccc                         >  1:807268/1‑150 (MQ=255)
                                                                                                                  cGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGcc                    >  2:647882/1‑150 (MQ=255)
                                                                                                                    tgtgCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCGTTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGCCaa                  <  2:1090779/150‑1 (MQ=255)
                                                                                                                    tgtgCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGCCaa                  <  2:1177256/150‑1 (MQ=255)
                                                                                                                              cTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGCCAAGCGTCGCAAc        <  2:28547/150‑1 (MQ=255)
                                                                                                                              cTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGCCAAGCGTCGCAAc        <  1:541687/150‑1 (MQ=255)
                                                                                                                                   cgaTGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGCCAAGCGTCGCAACCACtt   <  2:289348/150‑1 (MQ=255)
                                                                                                                                   cgaTGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGCCAAGCGTCGCAACCACtt   <  1:625650/150‑1 (MQ=255)
                                                                                                                                    gaTGGCCACCAGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGCCAAGCGTCGCAACCACTTc  >  1:538926/1‑150 (MQ=255)
                                                                                                                                    gaTGGCCACCAGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGCCAAGCGTCGCAACCACTTc  >  2:1224690/1‑150 (MQ=255)
                                                                                                                                            ccTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGa                                                                            >  2:924646/1‑68 (MQ=255)
                                                                                                                                            ccTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGa                                                                            <  1:924646/68‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                           
CTCGACCACCATACCGCCCAAGGCATCCAGGAGCTGATGCAGGAGCTGTCCAGCGCTTCGCGCACGGCATTCCTGGTGGTCACCCACGACCTCAACCTGGCGCGGCAGATGGACCGTGTGCTGAAGCTCGACGATGGCCACCTGGTGGCCATCTGATCGACTGCCGGGGTGGCAGGTGCTGCCCCGTTTTCCTGTTTCCATTGGGTGATTTCGTACATGTTCAGACCCTTGCCCATCTTTATCGGCGCGCGCTACACCCGAGCCAAGCGTCGCAACCACTTC  >  NC_002947/2462831‑2463112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑