Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,574,663 T→C 8.2% M459T (ATG→ACG)  PP_2257 → aerotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,574,6630TC 8.2% 128.4 / 2.2 49M459T (ATG→ACG) PP_2257aerotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (20/25);  new base C (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

TCATCGATGCCCTGCGCAGTGGCGCCGAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGCACAGCGCCCATAGCGCCAACGCCGCCGCCTCGCGCGGTACCGAACTGGAACACGT  >  NC_002947/2574516‑2574810
                                                                                                                                                   |                                                                                                                                                   
tcatcGATGCCCTGCGCAGTGGCGCCGAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGa                                                                                                                                                   <  1:501470/150‑1 (MQ=255)
    cGATGCCCTGCGCAGTGGCGCCGAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGCTGCTGTACAGCGCATCAGCGATATGAGCca                                                                                                                                               <  1:33735/150‑1 (MQ=255)
       tGCCCTGCGCAGTGGCGCCGAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCcagca                                                                                                                                            <  1:126677/150‑1 (MQ=255)
       tGCCCTGCGCAGTGGCGCCGAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCcagca                                                                                                                                            <  1:196066/150‑1 (MQ=255)
          ccTGCGCAGTGGCGCCGAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGAt                                                                                                                                         <  2:115434/150‑1 (MQ=255)
           cTGCGCAGTGGCGCCGAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATg                                                                                                                                        <  1:124814/150‑1 (MQ=255)
           cTGCGCAGTGGCGCCGAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATg                                                                                                                                        <  2:1106685/150‑1 (MQ=255)
                   tGGCGCCGAGGAAGCCGTGGTGATCGCCAGCCGAGGCATCGACGGTGAAGAGCAGGGCGTTGACCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGAGCATTAGCGATATGAGCCAGCAGATGGCCACCga                                                                                                                                >  2:88262/1‑149 (MQ=255)
                       gcCGAGGAAGCCGTGGCTATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCg                                                                                                                            >  2:656645/1‑150 (MQ=255)
                        ccGAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCgc                                                                                                                           >  1:256591/1‑150 (MQ=255)
                          gAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCgcag                                                                                                                         >  2:862904/1‑150 (MQ=255)
                                   ctGGCGATCGCCAGCGGAGGCATCGACGGTGCCGAGCAGGGCGGTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTcg                                                                                                                <  1:88262/149‑1 (MQ=255)
                                     ggCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGc                                                                                                                                >  1:266709/1‑132 (MQ=255)
                                     ggCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGc                                                                                                                                <  2:266709/132‑1 (MQ=255)
                                         aTCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTg                                                                                                          >  1:826265/1‑150 (MQ=255)
                                                         tCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCcagca                                                                                                                                            <  2:811060/100‑1 (MQ=255)
                                                         tCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCcagca                                                                                                                                            >  1:811060/1‑100 (MQ=255)
                                                         tCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTcgc                                                                                          <  2:873376/150‑1 (MQ=255)
                                                         tCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTcgc                                                                                          >  1:374102/1‑150 (MQ=255)
                                                            aCGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGc                                                                                       <  1:1285458/150‑1 (MQ=255)
                                                             cGGTGCCGAGCAGGGCGTTGCCCAGATTTATGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCa                                                                                      <  1:569/150‑1 (MQ=255)
                                                                tGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGAt                                                                                   <  2:15988/150‑1 (MQ=255)
                                                                    gAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCAc                                                                                                                                   <  1:36258/98‑1 (MQ=255)
                                                                    gAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCAc                                                                                                                                   >  2:36258/1‑98 (MQ=255)
                                                                    gAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATACGAGCCAGCAGATGGCCAc                                                                                                                                   >  2:36277/1‑98 (MQ=255)
                                                                    gAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATACGAGCCAGCAGATGGCCAc                                                                                                                                   <  1:36277/98‑1 (MQ=255)
                                                                         gggCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGttt                                                                                             <  2:290992/131‑1 (MQ=255)
                                                                         gggCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGttt                                                                                             >  1:290992/1‑131 (MQ=255)
                                                                          ggCGTTGCCCAGGTGCAGGAAGCTCGGCCGGCGCTGCCTGACATTCGTACCGCAGTGAAGAAGATAAAAAATATTGGTATGCGCATTGGCACCGCCTCGCATCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTa                                                                         >  2:786935/1‑150 (MQ=255)
                                                                              ttGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCg                                                                     <  2:536415/150‑1 (MQ=255)
                                                                              ttGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCg                                                                     <  1:778299/150‑1 (MQ=255)
                                                                                  ccAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGCACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGt                                                                                               <  2:1090419/120‑1 (MQ=255)
                                                                                  ccAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGCACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGt                                                                                               >  1:1090419/1‑120 (MQ=255)
                                                                                   cAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTAtt                                                                >  2:156176/1‑150 (MQ=255)
                                                                                       tGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGGTATTAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGTCGTAGCCGGTATTGccc                                                            <  2:174330/150‑1 (MQ=255)
                                                                                       tGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGccc                                                            <  2:826265/150‑1 (MQ=255)
                                                                                        gCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCa                                                           >  2:701728/1‑150 (MQ=255)
                                                                                         cAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATgg                                                                                                                                       >  1:1122278/1‑73 (MQ=255)
                                                                                         cAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATgg                                                                                                                                       <  2:1122278/73‑1 (MQ=255)
                                                                                          aGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGc                                                         >  2:711004/1‑150 (MQ=255)
                                                                                           ggAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGca                                                        <  1:757661/150‑1 (MQ=255)
                                                                                           ggAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATACGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTg                                                               >  1:893462/1‑143 (MQ=255)
                                                                                           ggAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATACGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTg                                                               <  2:893462/143‑1 (MQ=255)
                                                                                            gAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGa                                                                                                                                                   <  2:334082/58‑1 (MQ=255)
                                                                                            gAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGa                                                                                                                                                   >  1:334082/1‑58 (MQ=255)
                                                                                                                      tCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGCACAGCGCCCATAGCGCCAAcgccgccgc                             <  1:189167/150‑1 (MQ=255)
                                                                                                                         tGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGCACAGCGCCCATAGCGCCAACGCCGCCGCCTc                          >  1:260638/1‑150 (MQ=255)
                                                                                                                                     gcacATCAGCGATGTTAGCCAGCGGGTGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGCACCGCGCCCATAGCGCCAACGCCGCCGCCTCGCGCGGTACCGa              >  2:601943/4‑150 (MQ=255)
                                                                                                                                             gCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGCACAGCGCCCATAGCGCCAACGCCGCCGCCTCGCGCGGTACCGAACTGGAac      >  2:1126694/1‑150 (MQ=255)
                                                                                                                                                atatGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGCACAGCGCCCATAGCGCCAACGCCGCCGCCTCGCGCGGTACCGAACTGGAACACg   >  2:238648/1‑150 (MQ=255)
                                                                                                                                                atatGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGCACAGCGCCCATAGCGCCAACGCCGCCGCCTCGCGCGGTACCGAACTGGAACACg   >  2:900318/1‑150 (MQ=255)
                                                                                                                                                 tatGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGCACAGCGCCCATAGCGCCAACGCCGCCGCCTCGCGCGGTACCGAACTGGAACACgt  <  2:257686/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCATCGATGCCCTGCGCAGTGGCGCCGAGGAAGCCGTGGCGATCGCCAGCCGAGGCATCGACGGTGCCGAGCAGGGCGTTGCCCAGGTGCAGGAAGCTCGGCAGGCGCTGCATGGCATTCGTGACGCGGTACAGCGCATCAGCGATATGAGCCAGCAGATGGCCACCGCCTCGCAGCAGCAGTCGGCGGTGGCAGAAGACGTTTCGCGGCAGATCAACGGCGTAGCCGGTATTGCCCAGCACAGCGCCCATAGCGCCAACGCCGCCGCCTCGCGCGGTACCGAACTGGAACACGT  >  NC_002947/2574516‑2574810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑