| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 202,309 | C→G | 6.9% | V2605V (GTC→GTG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 202,309 | 0 | C | G | 6.9% | 160.9 / 5.1 | 58 | V2605V (GTC→GTG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (25/29); new base G (2/2); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.03e-01 | |||||||||||
GACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACT > NC_002947/202160‑202453 | gACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACAGCGACCCCTTCGGTc > 2:241138/1‑150 (MQ=255) cAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggg > 2:1273649/1‑149 (MQ=25) aGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAgggg < 1:1273649/150‑3 (MQ=25) aTCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGATTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGt < 2:415177/150‑1 (MQ=25) cAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTga < 1:917930/150‑1 (MQ=35) gCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAgg < 1:690164/142‑1 (MQ=25) gCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAgg > 2:690164/1‑142 (MQ=25) ccTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGAt > 1:1034659/1‑150 (MQ=34) gggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCGCCGAAGGCGGTGAGATCAc > 2:844392/1‑150 (MQ=18) gggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAc < 1:241138/150‑1 (MQ=33) gggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAc > 2:378935/1‑150 (MQ=33) ggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAcc < 2:182955/150‑1 (MQ=32) ggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAcc < 1:378935/150‑1 (MQ=32) aCGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACAGAAGGCGGTGAGATCACCTACAcc > 1:518405/1‑150 (MQ=17) gTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCa < 2:54026/109‑1 (MQ=32) gTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCa > 1:54026/1‑109 (MQ=33) aGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACcaacaa < 1:844392/150‑1 (MQ=32) gACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACaaa > 1:805710/1‑150 (MQ=32) tCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAc > 2:34673/1‑148 (MQ=32) tCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAc < 1:34673/148‑1 (MQ=33) acCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTg > 2:985412/1‑150 (MQ=35) acCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTg > 1:1237658/1‑150 (MQ=35) cTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGcc > 2:326235/1‑150 (MQ=35) cGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGAt < 2:938254/150‑1 (MQ=35) aCCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATcaaca < 1:326235/150‑1 (MQ=35) cTGTAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATc < 1:10963/114‑1 (MQ=17) cTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATc > 2:10963/1‑114 (MQ=32) cTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAACTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaacaac < 1:254926/150‑1 (MQ=25) tGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACAACGGGTTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAAcc < 1:403280/150‑1 (MQ=255) aaCTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATc > 2:82107/1‑140 (MQ=35) aaCTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATc < 1:82107/140‑1 (MQ=37) ggcggcAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACAGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAg < 2:518405/150‑1 (MQ=25) cggcAACGCTTCGGTTCAAGTGACCGACACCCTCGCCGAGGTGGTGGCGAAGGTGACCGCGACCCCTTCGGTCCTCATAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCt < 1:287965/150‑1 (MQ=11) aaCGCTTCGGTTCAAGTGACCGACACCTTCGACGAGGGGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAGCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTAc > 2:1154433/1‑150 (MQ=255) aaCGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTAc < 1:1279431/150‑1 (MQ=35) tCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAg < 1:1063776/150‑1 (MQ=35) cGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAGAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGc > 2:927188/1‑150 (MQ=25) cAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCgg > 2:1089474/1‑68 (MQ=37) cAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCgg < 1:1089474/68‑1 (MQ=37) cacCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAc < 2:646423/94‑1 (MQ=35) cacCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAc > 1:646423/1‑94 (MQ=35) ccTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgt > 1:1136966/1‑150 (MQ=38) cTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtc > 2:937710/1‑150 (MQ=39) tCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtcg > 1:423437/1‑150 (MQ=39) cgaGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtcgtcgt < 1:572288/150‑1 (MQ=39) ggCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCaa > 2:925645/1‑150 (MQ=38) gCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAAc < 1:985412/150‑1 (MQ=39) gAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaacaac < 1:582766/91‑1 (MQ=39) gAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaacaac > 2:582766/1‑91 (MQ=39) gAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACa > 2:1193856/1‑149 (MQ=39) gAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACa < 1:1193856/149‑1 (MQ=39) gAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAg > 1:809857/1‑150 (MQ=38) gACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGGCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGcaccac < 1:925645/150‑1 (MQ=38) gACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCaccacc < 2:928207/118‑1 (MQ=33) gACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCaccacc > 1:928207/1‑118 (MQ=33) gACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtcgtc > 2:1044895/1‑124 (MQ=34) gACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtcgtc < 1:1044895/124‑1 (MQ=33) tCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACt < 2:423437/150‑1 (MQ=32) tCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACt < 2:1034659/150‑1 (MQ=32) | GACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACT > NC_002947/202160‑202453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |