Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 240,531 G→C 8.7% A276P (GCC→CCC)  PP_0188 → uroporphyrin‑III C‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947240,5310GC 8.7% 135.6 / 5.5 46A276P (GCC→CCC) PP_0188uroporphyrin‑III C‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (20/22);  new base C (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

GTCCCCCGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGCAGACAAC  >  NC_002947/240383‑240680
                                                                                                                                                    |                                                                                                                                                     
gTCCCCCGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGc                                                                                                                                                      <  1:609604/150‑1 (MQ=255)
   ccccGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCgg                                                                                                                                                   <  1:1165151/150‑1 (MQ=255)
    cccGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCggg                                                                                                                                                  <  1:1069194/150‑1 (MQ=255)
     ccGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGCTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGgc                                                                                                                                                 <  2:861435/150‑1 (MQ=255)
           tCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGCCCGGCCAGCAAc                                                                                                                                           <  2:143065/150‑1 (MQ=255)
             gACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTg                                                                                                                                         >  1:1063503/1‑150 (MQ=255)
                    aCGCCTATGCGCTTGGCGCCCTGCACGCGGACGGTGCTGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGc                                                                                                                                  <  2:851603/150‑1 (MQ=255)
                                   gcgcCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGa                                                                                                                   <  2:302508/150‑1 (MQ=255)
                                     gcCTTGACCGCCGACGGTGATGGTGCCACCAGGCTGTCGCAGTGGTGGGCAGAACTATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGc                                                                                                                 >  2:1228009/1‑150 (MQ=255)
                                       ccTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCt                                                                                                               >  2:213945/1‑150 (MQ=255)
                                       ccTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGAAAGTTAACCAGCTGCGCCTGGCCCTGAGCCt                                                                                                               <  1:554708/150‑1 (MQ=255)
                                         tGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGGGGGGGGCAGAAATATCCAAGTACTTCCAGATCGACGTCAATGACGATGCAAACGTACGTCCCTTGCTGGCCGGGACCCAAGTGAACCACCTGCGCCTCGCCCTGAGCCCGa                                                                                                             >  2:245260/1‑150 (MQ=255)
                                          gACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGcca                                                                                                                      >  2:680216/1‑139 (MQ=255)
                                          gACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGcca                                                                                                                      <  1:680216/140‑2 (MQ=255)
                                            ccgccgACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGACAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTa                                                                                                          >  2:380586/1‑150 (MQ=255)
                                                   cGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCa                                                                                                   <  1:213945/150‑1 (MQ=255)
                                                     gTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAgg                                                                                                 >  2:1100957/1‑150 (MQ=255)
                                                                agcagGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGggcg                                                                                      >  2:855536/1‑150 (MQ=255)
                                                                agcagGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGggcg                                                                                      >  1:954/1‑150 (MQ=255)
                                                                        gTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCaa                                                                              <  1:1100957/150‑1 (MQ=255)
                                                                          cGCAGTGGTGGACAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATg                                                                            <  1:380586/150‑1 (MQ=255)
                                                                           gCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGc                                                                                                                                  <  1:626689/95‑1 (MQ=255)
                                                                           gCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGc                                                                                                                                  >  2:626689/1‑95 (MQ=255)
                                                                            cAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGt                                                                          <  2:1063503/150‑1 (MQ=255)
                                                                                     gCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAg                                                                 <  2:682989/150‑1 (MQ=255)
                                                                                       aGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGt                                                               >  2:277397/1‑150 (MQ=255)
                                                                                          aaTATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGAGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTa                                                            <  2:1142909/150‑1 (MQ=255)
                                                                                                 aaGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGc                                                                                                                                  <  2:1235759/73‑1 (MQ=255)
                                                                                                 aaGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGc                                                                                                                                  >  1:1235759/1‑73 (MQ=255)
                                                                                                       ttCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTg                                               >  2:23235/1‑150 (MQ=255)
                                                                                                       ttCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTg                                               <  1:277397/150‑1 (MQ=255)
                                                                                                         ccAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCAAAGGTGTACACGCAGGCGCTGga                                             >  1:390450/1‑150 (MQ=255)
                                                                                                          cAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGgac                                            <  2:390450/150‑1 (MQ=255)
                                                                                                            gATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGgacga                                          >  2:1239755/1‑150 (MQ=255)
                                                                                                            gATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGgacga                                          <  1:411557/150‑1 (MQ=255)
                                                                                                             aTCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGCGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCGCAAGGGTGATGCCAAGGTTTACACGCAGGCGCTGgacgac                                         <  1:1228009/150‑1 (MQ=255)
                                                                                                                   ttCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGAGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGc                                   >  1:1040786/1‑150 (MQ=255)
                                                                                                                     cAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAg                                 <  2:708782/150‑1 (MQ=255)
                                                                                                                            gATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCGGGGTCAGTATGCGGCGCTCAATGGTGATGCAACGGTTTACACACAGACGCTGGACTACGCCCGCAGCGTActg                          >  1:542579/1‑150 (MQ=255)
                                                                                                                              tGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATTGGTCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTActgct                        >  1:730706/1‑150 (MQ=255)
                                                                                                                                  aaCGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGcc                    >  1:741041/1‑150 (MQ=255)
                                                                                                                                     gTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAAc                 <  2:954/150‑1 (MQ=255)
                                                                                                                                      tGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACt                >  2:170582/1‑150 (MQ=255)
                                                                                                                                         ttcagTTGCTGCCCGGCCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCa             >  2:1058578/5‑150 (MQ=255)
                                                                                                                                            agTTGCTGCCCGGCCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATg          >  1:26610/2‑150 (MQ=255)
                                                                                                                                                gCTGCCCGGCCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGCAGa      <  1:1058578/150‑1 (MQ=255)
                                                                                                                                                    gCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGccc                                     >  2:616601/1‑115 (MQ=255)
                                                                                                                                                    gCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGccc                                     <  1:616601/115‑1 (MQ=255)
                                                                                                                                                    gCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCATGCCTCAAGGTCAGGTCCAGTGGGCGGCGCTCCATGGTTATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGCAGACAAc  <  1:926699/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTCCCCCGAGTTCGACAGTAACGCCGATGCGCTTGGCGCCCTGACCGCCGACGGTGATGGTGCCAGCAGGCTGTCGCAGTGGTGGGCAGAAATATCCAAGTACTTCCAGATCGACTTCAATGCCGATGAAAACGTGCGTCCGTTGCTGGCCGGGCAGCAACTGAACCAGCTGCGCCTGGCCCTGAGCCTGACTATCGAGCAGGCCCAGTGGGCGGCGCTCAATGGTGATGCCAAGGTTTACACGCAGGCGCTGGACGACGCCCGCAGCGTACTGCTGGCCAACTTCAATGCAGACAAC  >  NC_002947/240383‑240680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑