| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,149,977 | T→A | 8.9% | L13* (TTG→TAG) ‡ | PP_2766 → | long‑chain‑fatty acid‑‑CoA ligase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,149,977 | 0 | T | A | 8.9% | 164.4 / 7.7 | 56 | L13* (TTG→TAG) ‡ | PP_2766 | long‑chain‑fatty acid‑‑CoA ligase‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (27/24); new base A (3/2); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
TGCATTTCCAGGAAGAGGTCGAGTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTCGCGGCCGGCGA > NC_002947/3149831‑3150124 | tGCATTTCCAGGAAGAGGTCGAGTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCt > 1:559473/1‑150 (MQ=255) ggaaatgcAGGAAGAGGTCGAGTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTg < 1:38394/143‑1 (MQ=255) gCATTTCCAGGAAGAGGTCGAGTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTg < 2:837707/150‑1 (MQ=255) ccAGGAAGAGGTCGAGTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCGGGCCAGCAAGCTGGccggc > 1:1209778/1‑147 (MQ=255) ggAAGAGGTCGAGTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGTGca > 2:80268/1‑150 (MQ=255) gAAGAGGTCGAGTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGcc > 1:806243/1‑143 (MQ=255) gAAGAGGTCGAGTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGcc < 2:806243/143‑1 (MQ=255) gAGTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTg > 1:1106250/1‑150 (MQ=255) gTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTggg < 1:440484/150‑1 (MQ=255) ttCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCGGGCCAGCAAGCTGGccggca > 2:1017635/1‑128 (MQ=255) ttCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCGGGCCAGCAAGCTGGccggca < 1:1017635/132‑5 (MQ=255) ttCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGc > 1:1054998/1‑123 (MQ=255) ttCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGc < 2:1054998/123‑1 (MQ=255) cATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGAcgttgcgt > 1:986286/1‑150 (MQ=255) gtTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTgcgc < 1:1046245/150‑1 (MQ=255) cTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCtgt < 2:986286/150‑1 (MQ=255) cTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCtgt < 1:424279/150‑1 (MQ=255) gagcaggagcagCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGc > 2:961693/1‑150 (MQ=255) gcaggagcagCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAg > 2:520565/1‑150 (MQ=255) ggagcagCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑gcg > 2:395364/1‑150 (MQ=255) agcagcATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAgttgcg > 2:98966/1‑145 (MQ=255) caTGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCtgtg > 1:235797/1‑136 (MQ=255) caTGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCtgtg < 2:235797/136‑1 (MQ=255) cctccctcGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGgc > 2:1045571/1‑150 (MQ=255) cctccctcGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGgc < 1:845704/150‑1 (MQ=255) tccctcGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGgcgc > 2:812092/1‑150 (MQ=255) cctcGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCaa < 1:961693/150‑1 (MQ=255) ctcGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAAc > 1:575081/1‑150 (MQ=255) tcGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAGTTGCGCCCAGCTgcgcgcct < 1:98966/150‑3 (MQ=255) cTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAActgct < 2:1106250/150‑1 (MQ=255) gtgGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTgg < 1:520565/150‑1 (MQ=255) gCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCg > 1:197319/1‑150 (MQ=255) tGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAgg < 2:197319/150‑1 (MQ=255) gAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGt < 1:395364/150‑1 (MQ=255) gggTGCGCGGCCATGCATGAGGCGCTCGCGCACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTg < 2:302083/149‑1 (MQ=255) gcgcGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCaga > 1:1151610/1‑150 (MQ=255) ggCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGa > 1:455026/1‑138 (MQ=255) ggCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGa < 2:455026/138‑1 (MQ=255) gCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTg < 2:864134/150‑1 (MQ=255) ccATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGATTGCTGATCGGCCGATAACAACGTGGGGCGGACGGTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTgg > 1:864134/1‑150 (MQ=255) aGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAATCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGc > 1:1119225/1‑150 (MQ=255) cTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTc < 1:1045571/150‑1 (MQ=255) tGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGGTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACTGGGCTGAGCGAGGCCGGCTTcg > 1:1200457/1‑150 (MQ=255) tGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTcg > 1:1266729/1‑150 (MQ=255) cgggCCAGCAAGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCg > 2:116692/3‑106 (MQ=255) cgggCCAGCAAGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCg < 1:116692/104‑1 (MQ=255) ccGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTcgcg < 2:1151610/150‑1 (MQ=255) ccGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTcgcg > 2:1042220/1‑150 (MQ=255) ggCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCCCCGGGCTGCGCGAGGCCGGCTTCGcggc < 1:340340/150‑1 (MQ=255) ccAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTCGcggccg > 2:1222408/1‑150 (MQ=255) cAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAActg > 2:253671/1‑94 (MQ=255) cAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAActg < 1:253671/94‑1 (MQ=255) gCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTCGcggccggcc > 2:6602/1‑149 (MQ=255) gCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTCGCGGCCGGCg > 1:1192247/1‑150 (MQ=255) gCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTCGCGGCCGGCg > 1:838254/1‑150 (MQ=255) cTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTCGCGGCCGGCGa < 2:575081/150‑1 (MQ=255) | TGCATTTCCAGGAAGAGGTCGAGTACTTCGGCAAGCATGTGTTGCCGCTGGTGCGCGAACTGGAGCAGGAGCAGCATGCCTCCCTCGCTGTGGCCTGAGGTGCGCGGCCATGCATGAGGCGCTCGCGAACAACCTGCCGGCCAGCTTGCTGGCCCGGGCACATCAACGTGGGGCGGACGTTGCGTTGCGCCACAAGCGTCTAGGCCTGTGGCAG‑‑GCGCGCAGCTGGGCGCAACTGCTGGCCGAGGTGCAGAGCCTGGCCACCGGGCTGCGCGAGGCCGGCTTCGCGGCCGGCGA > NC_002947/3149831‑3150124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |