Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,309,743 C→T 7.4% A272V (GCG→GTG)  lhgO → L‑2‑hydroxyglutarate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,309,7430CT 7.4% 154.4 / 3.0 54A272V (GCG→GTG) lhgOL‑2‑hydroxyglutarate oxidase
Reads supporting (aligned to +/- strand):  ref base C (29/21);  new base T (2/2);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TGGCGAATACTACCTGCTGCCCAAGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGATCGAGATGAAGAACTCGCTGTTCAAGGGCG  >  NC_002947/3309594‑3309886
                                                                                                                                                      |                                                                                                                                               
tGGCGAATACTACCTGCTGCCCAAGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGc                                                                                                                                                 >  2:1152791/1‑150 (MQ=255)
  gCGAATACTACCTGCTGCCCAAGCAGCACAATTAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGa                                                                                                                                               >  2:975005/1‑150 (MQ=255)
  gCGAATACTACCTGCTGCCCAAGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGTGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACCCTCAGCT‑CGCGa                                                                                                                                               >  2:198915/1‑150 (MQ=255)
   cGAATACTACCTGCTGCCCAAGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGAt                                                                                                                                              >  2:196728/1‑150 (MQ=255)
     aaTACTACCTGCTGCCCAAGCAGCACAATCAGAGCGTCAACCACCTGATCTACCCGATTCCCGACCCGTAATTGCCCTTACTGGGCCTCAATCAGCCCCGCAGTTTCTATGGTACCGTCACCGTAGGCCCGAACGCTG‑GCTGGGCGATGa                                                                                                                                            >  1:792143/1‑150 (MQ=255)
          tacCTGCTGCCCAAGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAgcgc                                                                                                                                       <  1:1152791/150‑1 (MQ=255)
          tacCTGCTGCCCAAGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAgcgc                                                                                                                                       >  1:429377/1‑150 (MQ=255)
            ccTGCTGCCCAAGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGa                                                                                                                                     >  2:124108/1‑150 (MQ=255)
                    ccAAGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTAcc                                                                                                                             >  2:445753/1‑150 (MQ=255)
                      aaGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGc                                                                                                                           >  1:585663/1‑150 (MQ=255)
                          agcaCAATCAAATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGa                                                                                                                       <  1:850638/150‑1 (MQ=255)
                              caATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCT‑GGGGTGCACCTGACCCGCATGATCGACGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGAt                                                                                                                  <  1:618491/150‑1 (MQ=255)
                               aaTCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGAt                                                                                                                  <  2:74994/150‑1 (MQ=255)
                                     aTCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGc                                                                                                            <  2:429377/150‑1 (MQ=255)
                                           aaCCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCa                                                                                                              <  1:1004754/142‑1 (MQ=255)
                                           aaCCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCa                                                                                                              >  2:1004754/1‑142 (MQ=255)
                                               accTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAgc                                                                                                                                         >  1:630016/1‑111 (MQ=255)
                                               accTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAgc                                                                                                                                         <  2:630016/111‑1 (MQ=255)
                                                ccTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGa                                                                                                                                     <  1:741415/114‑1 (MQ=255)
                                                ccTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGa                                                                                                                                     >  2:741415/1‑114 (MQ=255)
                                                  tGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGaa                                                                                                                                           >  1:744773/1‑106 (MQ=255)
                                                  tGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGaa                                                                                                                                           <  2:744773/106‑1 (MQ=255)
                                                    aTCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTc                                                                                             <  1:198915/150‑1 (MQ=255)
                                                       tACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAg                                                                                          >  2:458623/1‑150 (MQ=255)
                                                        aCCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGa                                                                                         >  1:786251/1‑150 (MQ=255)
                                                            gATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCCTGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCt                                                                                     <  2:786251/150‑1 (MQ=255)
                                                                 ccGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGTGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCa                                                                                >  1:1127869/1‑150 (MQ=255)
                                                                 ccGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGTGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCa                                                                                >  1:1127999/1‑150 (MQ=255)
                                                                           gATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGAt                                                                                                                  >  1:1190111/1‑106 (MQ=255)
                                                                           gATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGAt                                                                                                                  <  2:1190111/106‑1 (MQ=255)
                                                                                ccTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCa                                                                 >  1:252706/1‑150 (MQ=255)
                                                                                 cTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCaa                                                                <  1:458623/150‑1 (MQ=255)
                                                                                        ggggTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTg                                                         >  1:249833/1‑150 (MQ=255)
                                                                                             gCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGTGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCgaa                                                    <  2:1127869/150‑1 (MQ=255)
                                                                                             gCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGTGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCgaa                                                    <  2:1127999/150‑1 (MQ=255)
                                                                                             gCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCgaa                                                    <  2:792143/150‑1 (MQ=255)
                                                                                                 cTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCa                                                                                                        >  2:913091/1‑94 (MQ=255)
                                                                                                 cTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCa                                                                                                        <  1:913091/94‑1 (MQ=255)
                                                                                                   gACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGAt                                                                                                                  >  1:991996/1‑82 (MQ=255)
                                                                                                   gACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGAt                                                                                                                  <  2:991996/82‑1 (MQ=255)
                                                                                                        gCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGcc                                         >  1:657669/1‑150 (MQ=255)
                                                                                                              tCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCgggac                                   >  2:1102291/1‑148 (MQ=255)
                                                                                                                gATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGAccc                                                                                      >  2:278292/1‑97 (MQ=255)
                                                                                                                gATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGAccc                                                                                      <  1:278292/97‑1 (MQ=255)
                                                                                                                 aTGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGa                                >  2:285455/1‑150 (MQ=255)
                                                                                                                  tGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGAt                               <  1:975005/150‑1 (MQ=255)
                                                                                                                   ggCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGATc                              <  1:445753/150‑1 (MQ=255)
                                                                                                                          tcaccgtAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGATCGAGATga                       >  1:982674/1‑150 (MQ=255)
                                                                                                                             ccgtAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGATCGAGATgaaga                    >  2:1101856/1‑150 (MQ=255)
                                                                                                                              cgtAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGATCGAGATgaagaa                   >  1:615592/1‑150 (MQ=255)
                                                                                                                              accaGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGCCGAAGAACTTCCGCCCGGGCCTGATCGAGATgaggaa                   >  1:887597/4‑150 (MQ=255)
                                                                                                                                tAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGATCGAGATgaagtaaa                 >  2:71654/1‑146 (MQ=255)
                                                                                                                                    ccccAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGATCGAGATGAAGAACTCGCt             <  1:124108/150‑1 (MQ=255)
                                                                                                                                           gCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCAAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGATCGAGATGAAGAACTCGCTGTTCAAg      >  1:1055836/1‑150 (MQ=255)
                                                                                                                                               tgCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGATCGAGATGAAGAACTCGCTGTTCAAGGGCg  <  1:196728/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                               
TGGCGAATACTACCTGCTGCCCAAGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCCGTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTAGGCCCCAACGCTGTGCT‑GGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCCCCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAACTTCCGCCCGGGCCTGATCGAGATGAAGAACTCGCTGTTCAAGGGCG  >  NC_002947/3309594‑3309886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑