| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,673,954 | C→G | 7.0% | A227G (GCC→GGC) | PP_4137 → | outer membrane siderophore receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,673,954 | 0 | C | G | 7.0% | 177.7 / 5.1 | 60 | A227G (GCC→GGC) | PP_4137 | outer membrane siderophore receptor |
| Reads supporting (aligned to +/- strand): ref base C (28/27); new base G (2/2); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01 | |||||||||||
TACGAAGTCGGTAGCCGCGGCTATCAGCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGCCAACCCCAACTATCTGGCACGCGAC > NC_002947/4673809‑4674102 | tACGAAGTCGGTAGCCGCGGCTATCAGCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAAt > 2:225169/1‑150 (MQ=255) aaGTCGGTAGCCGCGGCTATCAGCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCg < 2:1249508/150‑1 (MQ=255) aaGTCGGTAGACGCGGCTATCAGCATCGGCGTATCGGGTCGGGCCAGGTGCTCGGCAACCTTGACTACGCACTGGGCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCg < 1:863314/150‑1 (MQ=255) aGTCGGTAGCCGCGGCTATCAGCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCgg < 2:729090/150‑1 (MQ=255) gTCGGTAGCCGCGGCTATCAGCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGt > 1:1243797/1‑150 (MQ=255) gCCGCGGCTATCAGCACCGCGTCGTCAGTTCGAGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGCCGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCt < 1:707219/150‑1 (MQ=255) ccGCGGCTATCAGCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCtt < 2:106605/150‑1 (MQ=255) tCAGCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCaa < 1:1075137/150‑1 (MQ=255) aGCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAAcc > 1:815962/1‑150 (MQ=255) aGCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAAcc < 1:728751/150‑1 (MQ=255) gCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCtt > 2:1077099/1‑138 (MQ=255) gCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCtt < 1:1077099/138‑1 (MQ=255) aacAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGc > 1:686247/3‑150 (MQ=255) taaGTTCGGGCCAGGAGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCt > 2:119051/3‑150 (MQ=255) tCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCt > 1:428330/1‑150 (MQ=255) ggCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTg > 1:492531/1‑150 (MQ=255) ggCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTg < 1:225169/150‑1 (MQ=255) gCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTgc > 2:525666/1‑150 (MQ=255) gCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTgc > 1:1002806/1‑150 (MQ=255) ccAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTgcg > 1:435935/1‑150 (MQ=255) ccAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTgcg < 2:686247/150‑1 (MQ=255) gTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGGCGGCAATGcccttg < 1:351631/106‑5 (MQ=255) gTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGGCGGCAATGcccttg > 2:351631/1‑102 (MQ=255) cTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCgg < 2:815962/150‑1 (MQ=255) gCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGaaa > 2:694901/1‑150 (MQ=255) ttGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGaaa > 1:957593/1‑150 (MQ=255) ttGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGaaa > 2:431382/1‑150 (MQ=255) gACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAAc < 2:428330/150‑1 (MQ=255) tATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGaa < 1:525666/150‑1 (MQ=255) gTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGccc < 2:492531/150‑1 (MQ=255) tGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCg > 1:87485/1‑150 (MQ=255) ccTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGgccg > 1:1018822/1‑150 (MQ=255) gACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGaaaa > 1:911052/1‑132 (MQ=255) gACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGaaaa < 2:911052/132‑1 (MQ=255) gACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCt > 2:1001432/1‑150 (MQ=255) cGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGAc > 1:98129/1‑150 (MQ=255) aTTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCa > 1:906146/1‑150 (MQ=255) ttCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCaa < 2:1018822/150‑1 (MQ=255) ttCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCaa < 2:98129/150‑1 (MQ=255) cgCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCAGTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAATGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAg < 1:457882/149‑1 (MQ=255) cAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGGCGGCAATGccc < 2:1020701/46‑2 (MQ=25) cAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGGCGGCAATGccc > 1:1020701/1‑45 (MQ=25) cAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACCGGGTTCCCGGCCGCCTGACCAAGGACCAGATCAAGcaccac > 1:655536/1‑150 (MQ=255) cAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCCAGAATGCTCGC‑CCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGcaccac < 2:1008617/150‑1 (MQ=255) gcACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGcaccac < 2:1225532/145‑1 (MQ=255) gcACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGcaccac > 1:1225532/1‑145 (MQ=255) acaGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGaaaa > 2:1155042/1‑100 (MQ=255) acaGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGaaaa < 1:1155042/100‑1 (MQ=255) agcagcGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCcgcgcgcg > 1:878911/1‑150 (MQ=255) cagcggcagcgCCAAGGGCATTGCCGCCAATGCCTGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTTAAATCGAACTGCCCGGCCGCCTGACCAAGGACCATATCAAGCACCACCCGCGCGCGGc < 2:496033/150‑1 (MQ=255) cagcggcagcgCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGc < 2:87485/150‑1 (MQ=255) gcagcgCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGa < 1:189627/123‑1 (MQ=255) gcagcgCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGa > 2:189627/1‑123 (MQ=255) agcgCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGCCAAcccc < 2:878911/150‑1 (MQ=255) gggCATTGCCGCCAATGCCGGTTACCGCTTCACCCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGCCAACCCCAACTATCt > 1:517045/1‑150 (MQ=255) gggCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGCCAACCCCAACTATCt < 1:119051/150‑1 (MQ=255) gggCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGCCAACCCCAACTATCt < 2:1002806/150‑1 (MQ=255) ggCATTGCCGTTAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGCCAACCCCAACTATCTg < 1:694901/150‑1 (MQ=255) aTTGCCGCCAATGCCGGTTACCACTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGCCAACCCCAACTATCTGGCa > 2:920148/1‑150 (MQ=255) ttGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGCCAACCCCAACTATCTGGCAc > 1:995929/1‑150 (MQ=255) gccAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGCCAACCCCAACTATCTGGCACGcgac > 2:933223/1‑150 (MQ=255) | TACGAAGTCGGTAGCCGCGGCTATCAGCATCGGCACATCAGTTCGGGCCAGGTGCTGGGCAACCTTGACTATTACGTGGCCCTGACCGATTCGGAATATGACGGCTACCAGGCGCACAGCAGCGGCAGCGCCAAGGGCATTGCCGCCAATGCCGGTTACCGCTTCAACCCCAACCTGGAA‑ACCCGCTTCTACCTGCGCTATCGGGAAACTGAAAACGAACTGCCCGGCCGCCTGACCAAGGACCAGATCAAGCACCACCCGCGCGCGGCCAACCCCAACTATCTGGCACGCGAC > NC_002947/4673809‑4674102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |