| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,832,113 | C→G | 7.3% | intergenic (+43/+109) | pvdS → / ← pvdY | alternative sigma factor/hydroxyproline acetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,832,113 | 0 | C | G | 7.3% | 170.2 / 5.1 | 55 | intergenic (+43/+109) | pvdS/pvdY | alternative sigma factor/hydroxyproline acetylase |
| Reads supporting (aligned to +/- strand): ref base C (24/27); new base G (2/2); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01 | |||||||||||
GGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAACCG > NC_002947/4831971‑4832251 | ggACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTttg > 2:1040587/1‑150 (MQ=255) tGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAAAGCGAGAGCGATCCAGGTTCGCTCTGGCGTTTGCTTGCa < 1:521896/150‑1 (MQ=37) ccAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAgc > 1:1047022/1‑150 (MQ=255) ccAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAgc > 2:814224/1‑150 (MQ=255) aaGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAgcgc < 2:422490/150‑1 (MQ=255) aaGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAgcgc < 1:814224/150‑1 (MQ=255) aGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCAGCGATGAGATGAAGCTATGCCGCAAGACGTATTGTCTCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCt > 1:1121221/1‑150 (MQ=255) ggAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTa > 2:991664/1‑150 (MQ=255) cTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTAt > 1:196822/1‑150 (MQ=255) aTCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGtttt < 2:992456/150‑1 (MQ=255) ttGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACgg > 2:790732/1‑150 (MQ=255) tGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGc < 1:1089134/150‑1 (MQ=255) tGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTCATACAAAACGCCAGAGTGAAGCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGc < 1:798253/150‑1 (MQ=255) ttCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCCGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACt < 1:1040587/150‑1 (MQ=255) gATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAg > 2:204539/1‑150 (MQ=255) tCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATaa < 1:516774/141‑1 (MQ=255) tCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATaa > 2:516774/1‑141 (MQ=255) gcgATGCGCTGGTGCATTGCCGCCAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCaa > 1:305015/1‑150 (MQ=255) aTGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCaaaaa > 2:1116411/1‑150 (MQ=255) gcgcTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCATCGCAAAAAAc < 2:1114179/150‑1 (MQ=255) gcgcTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAAc < 1:1146229/150‑1 (MQ=255) gcgcTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAAc < 1:538861/150‑1 (MQ=255) aTTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGtt < 2:1047022/150‑1 (MQ=255) aTTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGtt > 2:849377/1‑150 (MQ=255) gCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCGATCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATg < 1:336786/150‑1 (MQ=255) gCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATg < 1:285100/150‑1 (MQ=255) gCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATg < 1:284671/150‑1 (MQ=255) gCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATg < 1:849377/150‑1 (MQ=255) cGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGt > 1:919236/1‑150 (MQ=255) gCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGc < 1:616648/103‑1 (MQ=255) gCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGc > 2:616648/1‑103 (MQ=255) ccAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtgt < 1:204539/150‑1 (MQ=255) ccAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtgt < 2:196822/150‑1 (MQ=255) ccAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtgt < 1:49308/150‑1 (MQ=255) cAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtgtg < 2:602839/150‑1 (MQ=255) aGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGa > 2:446271/1‑150 (MQ=255) aGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAAGACGGCCACTAAAGTTCTGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGa < 2:1008988/150‑1 (MQ=255) ccAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAacac > 1:175178/1‑150 (MQ=255) aGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCCAGTACGGTGTGTTGCAAATACGGCCAATAACACGCAGCGCAAAAGACGCGGCACGTTATGGTCGTTATTTGTGAAAcacca > 1:1242146/1‑150 (MQ=255) gCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACcaca < 1:790732/150‑1 (MQ=255) cTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAgcgc > 1:314484/1‑105 (MQ=255) cTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTGGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAgcgc < 2:314484/105‑1 (MQ=255) gcaaACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCt < 1:1116411/150‑1 (MQ=255) aaaagcgagagCGATCCAGGTTCGCTCTGGCGTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTg > 1:952032/8‑150 (MQ=38) aaCGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTAttt < 1:1261765/116‑1 (MQ=255) aaCGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTAttt > 2:1261765/1‑116 (MQ=255) aagcgagagCGATCCAGGTTCGCTCTGGCGTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtgt < 2:356095/112‑1 (MQ=37) aagcgagagCGATCCAGGTTCGCTCTGGCGTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtgt > 1:356095/6‑117 (MQ=25) cGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCaa < 1:653391/150‑1 (MQ=255) agagCGAACCTGGATCGCTCTCGCTGTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGAt > 1:1183366/1‑150 (MQ=255) gagCGAACCTGGATCGCTATCGCTTTTGCTTGCAACGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTAGCAGCGCAAAAAACGCCGCAAGGTATGGTCGTTATTTGTGAAACACCACACAAGCCCCTACGTCAGCTTACAACGATc > 2:414830/1‑150 (MQ=255) gCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGa > 1:1167861/1‑150 (MQ=255) gAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGaaa < 2:175178/150‑1 (MQ=255) aCCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACGCCACACTGTCCCCTACGGCAGCTTGCAACGATCGaaaaa > 2:851700/1‑150 (MQ=255) tGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAACCg > 1:501319/1‑150 (MQ=255) tctcGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAAcgcc > 1:1210252/1‑72 (MQ=255) tctcGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAAcgcc < 2:1210252/72‑1 (MQ=255) tcGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAAc < 1:328102/51‑1 (MQ=255) tcGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAAc > 2:328102/1‑51 (MQ=255) | GGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAACCG > NC_002947/4831971‑4832251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |