Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 36,677 G→C 8.9% P326A (CCA→GCA)  PP_0033 ← undecaprenyl‑glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294736,6770GC 8.9% 165.1 / 6.2 55P326A (CCA→GCA) PP_0033undecaprenyl‑glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (23/27);  new base C (2/3);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CCGCTGATTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAACAAGGCCCATGG  >  NC_002947/36528‑36824
                                                                                                                                                     |                                                                                                                                                   
ccGCTGATTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGTAGACAGCAGCAATGCCCACGCTgg                                                                                                                                                     <  1:598054/150‑1 (MQ=255)
      aTTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAgcgc                                                                                                                                               <  2:745874/150‑1 (MQ=255)
        tCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCACACTTTTCTGACCCGTGCAGACAGCAGAAATGCCCACGCTGGCAGCGCgg                                                                                                                                             <  1:1258796/150‑1 (MQ=255)
         atctgggCGCTCTTCCAGGACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCggg                                                                                                                                            <  2:873983/146‑1 (MQ=255)
           gAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTa                                                                                                                                          <  1:995930/150‑1 (MQ=255)
            aGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCGCGCTGCCAGCGTgggcat                                                                                                                                         <  1:51318/150‑4 (MQ=255)
              ggCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGGGACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAgat                                                                                                                                       >  1:873983/1‑148 (MQ=255)
                     ctctAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCaggtaggt                                                                                                                                >  1:425762/1‑150 (MQ=255)
                                 ggCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGtt                                                                                                                    <  1:745341/150‑1 (MQ=255)
                                   cGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGc                                                                                                                  <  1:1004812/150‑1 (MQ=255)
                                   cGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGc                                                                                                                  <  1:549450/150‑1 (MQ=255)
                                     tAGATGATGCCCGCACCGATGATTGGCAACACCTATGCTCGCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCt                                                                                                                <  2:425762/150‑1 (MQ=255)
                                     tAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCt                                                                                                                <  2:709349/150‑1 (MQ=255)
                                       gatgatGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGc                                                                                                              >  2:483794/1‑150 (MQ=255)
                                           atgCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAt                                                                                                          <  1:1167794/150‑1 (MQ=255)
                                             gCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtgt                                                                                                        <  2:1087561/150‑1 (MQ=255)
                                               ccGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTga                                                                                                      >  1:61223/1‑150 (MQ=255)
                                                cGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTgaa                                                                                                     >  2:1285087/1‑150 (MQ=255)
                                                       atgatTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGAcc                                                                                              <  2:1005756/150‑1 (MQ=255)
                                                                  cacCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCaa                                                                                   <  1:146736/150‑1 (MQ=255)
                                                                   acCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGcgcg                                                                                                                                              >  1:2980/1‑90 (MQ=255)
                                                                   acCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGcgcg                                                                                                                                              <  2:2980/90‑1 (MQ=255)
                                                                     cAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTCCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGc                                                                                >  1:1096552/1‑150 (MQ=255)
                                                                     cAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGACAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACACAGCATTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGc                                                                                >  1:337330/1‑150 (MQ=255)
                                                                               gCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTga                                                                                                      <  1:4100/118‑1 (MQ=255)
                                                                               gCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTga                                                                                                      >  2:4100/1‑118 (MQ=255)
                                                                               gCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCag                                                                      >  1:815792/1‑150 (MQ=255)
                                                                                cAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCaga                                                                     <  2:127678/150‑1 (MQ=255)
                                                                                         cccAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCtt                                                            >  1:600760/1‑150 (MQ=255)
                                                                                          ccAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTa                                                                 >  2:560319/1‑144 (MQ=255)
                                                                                          ccAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTa                                                                 <  1:560319/144‑1 (MQ=255)
                                                                                          ccAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGACCACAGCCACAGGTAGGCgtt                                                           >  2:397694/1‑150 (MQ=255)
                                                                                           cAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCca                                                                                                                             <  1:737089/83‑1 (MQ=255)
                                                                                           cAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCca                                                                                                                             >  2:737089/1‑83 (MQ=255)
                                                                                                 gtCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAgcgc                                                    >  2:628795/1‑150 (MQ=255)
                                                                                                    ttCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGc                                                 >  1:1130521/1‑150 (MQ=255)
                                                                                                    ttCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGc                                                 >  1:305599/1‑150 (MQ=255)
                                                                                                     tCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCa                                                <  1:628795/150‑1 (MQ=255)
                                                                                                         gCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtgt                                                                                                        >  2:1135624/1‑90 (MQ=255)
                                                                                                         gCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtgt                                                                                                        <  1:1135624/90‑1 (MQ=255)
                                                                                                              cTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCAc                                       >  2:1048337/1‑150 (MQ=255)
                                                                                                               ttCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACg                                      >  2:584082/1‑150 (MQ=255)
                                                                                                                     gACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCg                                <  1:483794/150‑1 (MQ=255)
                                                                                                                     gACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCg                                <  1:397694/150‑1 (MQ=255)
                                                                                                                      aCCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCgg                               <  1:1048337/150‑1 (MQ=255)
                                                                                                                             cAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtg                                                                                                         <  1:978153/69‑1 (MQ=255)
                                                                                                                             cAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtg                                                                                                         >  2:978153/1‑69 (MQ=255)
                                                                                                                                   gcagcaATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTa                  <  2:305599/150‑1 (MQ=255)
                                                                                                                                     accaATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGa                >  2:960793/3‑150 (MQ=255)
                                                                                                                                        aaTGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAAc             <  2:61223/150‑1 (MQ=255)
                                                                                                                                          tGCCCACGCTGGCAGCGCGGGTAGCAAGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAACaa           >  1:261448/1‑150 (MQ=255)
                                                                                                                                           acccgcGCTGCCAGCGTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTg                     >  2:385611/6‑139 (MQ=255)
                                                                                                                                           acccgcGCTGCCAGCGTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTg                     <  1:385611/134‑1 (MQ=255)
                                                                                                                                            cccgcGCTGCCAGCGTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCtt                                   <  2:1245228/120‑1 (MQ=255)
                                                                                                                                            cccGCGCTGCCAGCGTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCtt                                   >  1:1245228/1‑124 (MQ=255)
                                                                                                                                                   tGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAACAAGGCCCATgg  <  1:960793/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCGCTGATTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAACAAGGCCCATGG  >  NC_002947/36528‑36824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑