| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 326,067 | C→A | 10.5% | *556Y (TAG→TAT) | PP_0269 ← | glutamate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 326,067 | 0 | C | A | 10.5% | 168.6 / 5.9 | 55 | *556Y (TAG→TAT) | PP_0269 | glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (26/23); new base A (2/4); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TCGATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCA > NC_002947/325920‑326211 | tCGATCAAGCTGAGTAGCTCGCTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTa > 2:1078164/1‑150 (MQ=255) gATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATg > 2:1248673/1‑150 (MQ=255) gCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCgggg < 2:956506/150‑1 (MQ=255) gCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCgggg > 2:764816/1‑150 (MQ=255) gCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCgggg < 2:299782/150‑1 (MQ=255) gCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCGGGGCTg < 2:352678/150‑1 (MQ=255) cGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGt < 1:294332/150‑1 (MQ=255) tAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCGGGGCTGttc < 1:764816/150‑2 (MQ=255) cTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtt > 1:384542/1‑150 (MQ=255) ttCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCg > 1:345498/1‑150 (MQ=255) caacaaCGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTa > 1:332843/1‑150 (MQ=255) caacaaCGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTa > 1:449551/1‑150 (MQ=255) aacaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTAc > 2:1098370/1‑150 (MQ=255) aacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGt > 2:238191/1‑150 (MQ=255) tGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGc > 2:271094/1‑116 (MQ=255) tGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGc < 1:271094/116‑1 (MQ=255) agcagAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGgc < 2:449551/150‑1 (MQ=255) aaCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTgg < 2:332843/150‑1 (MQ=255) aaCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTgg < 2:345498/150‑1 (MQ=255) acgGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATTAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGt < 1:1173634/150‑1 (MQ=255) ggCAACGAAGTCCGCTTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGc > 1:670799/1‑150 (MQ=255) gAAGTCCGCGTATTCGTCGAGTACCCGCTCCGCCTCATCTCATGTTCAGCTGATGTATCGAACAGCCCCGGCATGTGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCt < 1:1248673/150‑1 (MQ=255) gAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAAAAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCCGCGCTGGGCTTACGGTCTCCGGTGCAAAGCt < 2:788120/150‑1 (MQ=255) tCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCTCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCg > 2:815831/1‑150 (MQ=255) cgcgTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTc > 1:639503/1‑147 (MQ=255) cgcgTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTc < 2:639503/147‑1 (MQ=255) cgcgTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCt > 1:1266854/1‑150 (MQ=255) gcgTATTCGTAGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTa < 2:493288/150‑1 (MQ=255) tATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGATTAAGCTTTTCTGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTAcgc > 1:156586/1‑150 (MQ=255) cgtcgAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCa < 1:1098370/150‑1 (MQ=255) gtcgAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACg < 2:296868/118‑1 (MQ=255) gtcgAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACg > 1:296868/1‑118 (MQ=255) gtcgAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCAt > 2:75709/1‑150 (MQ=255) tcgAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATc > 2:457701/1‑150 (MQ=255) gATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCaaa > 1:227268/1‑121 (MQ=255) gATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCaaa < 2:227268/121‑1 (MQ=255) aGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTAcgc > 2:1262260/1‑130 (MQ=255) aGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTAcgc < 1:1262260/130‑1 (MQ=255) ctgatctgatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCttt < 1:238191/150‑1 (MQ=255) ctgatctgatGTAGCGAACAGCCCCGGGCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCGCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCttt < 1:393733/150‑1 (MQ=255) ttatctgatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtc < 1:396076/148‑1 (MQ=255) tctgatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgat > 2:1102036/1‑150 (MQ=255) atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAg > 2:454790/1‑150 (MQ=255) atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAg > 1:213174/1‑150 (MQ=255) tGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGc < 2:213174/150‑1 (MQ=255) tATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTAcgc > 1:513728/1‑105 (MQ=255) tATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTAcgc < 2:513728/105‑1 (MQ=255) aCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGt > 2:403986/1‑150 (MQ=255) aCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCGCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGt < 2:1266854/150‑1 (MQ=255) aCAGCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGt > 2:1113190/1‑150 (MQ=255) aGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTaa > 1:477805/1‑150 (MQ=255) aGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTaa < 2:477805/150‑1 (MQ=255) ccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCg < 2:63323/52‑1 (MQ=255) ccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCg > 1:63323/1‑52 (MQ=255) ccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCa < 1:457701/150‑1 (MQ=255) | TCGATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCA > NC_002947/325920‑326211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |