| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,870,087 | G→A | 6.7% | V598M (GTG→ATG) | xdhB → | xanthine dehydrogenase subunit XdhB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,870,087 | 0 | G | A | 6.7% | 168.8 / 2.9 | 60 | V598M (GTG→ATG) | xdhB | xanthine dehydrogenase subunit XdhB |
| Reads supporting (aligned to +/- strand): ref base G (26/30); new base A (2/2); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01 | |||||||||||
TCCTCAAGAAACGCCTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATCGTCGATACGCTGACCGGCGAATACAA > NC_002947/4869944‑4870229 | tCCTCAAGAAACGCCTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGAACAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGc > 1:214235/1‑150 (MQ=255) ccTCAAGAAACGCCTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCt < 2:662960/150‑1 (MQ=255) tCAAGAAACGCCTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGt > 1:302910/1‑150 (MQ=255) tCAAGAAACGCCTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGt > 1:148162/1‑150 (MQ=255) tCAAGAAACGCCTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGATGTCGCTGt > 2:154458/1‑150 (MQ=255) tCAAGAAACGCCTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGATGTCGCTGt > 2:154509/1‑150 (MQ=255) aaGAAACGCCTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTcc > 2:413254/1‑150 (MQ=255) ccTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGc > 2:667736/1‑145 (MQ=255) ccTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGc < 1:667736/145‑1 (MQ=255) aCCGAGTTTGCCGCACGCCACTAGCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGACATGTGCGGGTGCGCGACCAGATCGTCAGATTCGAATAAC‑TGTGGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTc < 1:349782/150‑1 (MQ=255) cGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTa < 2:834395/150‑1 (MQ=255) cGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTa > 1:1123344/1‑150 (MQ=255) gAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGCACGTCAGCTTCGATCAGCGTGGT‑CGTCAGGCCCACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTAc < 1:24125/150‑1 (MQ=255) tttGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGc > 2:133047/1‑150 (MQ=255) cGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGcc > 1:721697/1‑150 (MQ=255) aCGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGaa < 2:3013/150‑1 (MQ=255) aCTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGAtct > 1:581599/1‑150 (MQ=255) tCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGATGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTa < 1:154509/150‑1 (MQ=255) tCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGATGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTa < 1:154458/150‑1 (MQ=255) cGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGt < 1:1030538/116‑1 (MQ=255) cGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGt > 2:1030538/1‑116 (MQ=255) cGAGGAAGACGTCGAATCTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAg > 2:761411/1‑150 (MQ=255) aCGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCAc > 1:1177397/1‑118 (MQ=255) aCGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCAc < 2:1177397/118‑1 (MQ=255) aCGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGccc > 2:1093159/1‑150 (MQ=255) aaCGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTc < 2:1123344/150‑1 (MQ=255) aaCGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTc > 2:83343/1‑150 (MQ=255) cATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAAATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCtactac > 1:276863/1‑150 (MQ=255) aTGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCtactacg < 2:880712/150‑2 (MQ=255) tgtgCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCTGGTGTCGCAGTCCACTTCCGGGTTCTACCGCACGCCGAACACCTTCTACGTCCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACtt < 1:1253725/150‑1 (MQ=255) gtgCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTc < 2:302910/150‑1 (MQ=255) gtgCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTc > 2:728829/1‑150 (MQ=255) gTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCttt < 2:409125/150‑1 (MQ=255) tGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTg < 2:1274600/150‑1 (MQ=255) cgcgACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGgc < 1:1070678/150‑1 (MQ=255) gcgACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCGTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGgcg > 2:604012/1‑150 (MQ=255) ccAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGctgc < 1:761411/150‑1 (MQ=255) gATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGAtcttc < 1:670226/91‑1 (MQ=255) gATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGAtcttc > 2:670226/1‑91 (MQ=255) catccgCTTCGAGCAAC‑TGGCGCAGCGCGCCTACGTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGTCCGCGGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGt < 2:276863/145‑1 (MQ=255) gTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCTCTGTCTGCGTg < 2:148162/150‑1 (MQ=255) cAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGgcg < 2:131758/137‑1 (MQ=255) cAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGgcg > 1:131758/1‑137 (MQ=255) ttCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTg > 2:493801/1‑150 (MQ=255) ttCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCGTTGGCGCTGCCTGCGTGGAAGTg > 2:1184097/1‑150 (MQ=255) gAGCAAC‑TGGTGCAGCAGGCCTAGTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGAtc < 2:196525/150‑1 (MQ=255) aC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCcgcg > 2:491949/1‑95 (MQ=255) aC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCcgcg < 1:491949/95‑1 (MQ=255) aC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGcc < 2:652610/118‑1 (MQ=255) aC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGcc > 1:652610/1‑118 (MQ=255) aC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATCGTCGa < 2:581599/150‑1 (MQ=255) gTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATCGTCGATACg > 2:439551/1‑150 (MQ=255) agcagGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATCGTCGATACGCTGa < 2:641191/150‑1 (MQ=255) agcagGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATCGTCGATACGCTGa < 2:721697/150‑1 (MQ=255) agcagGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATCGTCGATACGCTGa > 1:194715/1‑150 (MQ=255) agcagGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATCGTCGATACGCTGa < 1:1093159/150‑1 (MQ=255) gcagGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCtt > 2:735317/1‑111 (MQ=255) gcagGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCtt < 1:735317/111‑1 (MQ=255) gCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTCGAAGTGATCGTCGATACGCTGACCGGc < 2:194715/150‑1 (MQ=255) cGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATCGTCGATACGCTGACCGGCGAATACaa < 1:439551/150‑1 (MQ=255) | TCCTCAAGAAACGCCTGACCGAGTTTGCCGCACGGCACTATCAAGTGACCGAGGAAGACGTCGAATTTCGCAACGGCCATGTGCGGGTGCGCGACCAGATCGTCAGCTTCGAGCAAC‑TGGTGCAGCAGGCCTACTTCGCCCAGGTGTCGCTGTCCAGCACCGGGTTCTACCGCACGCCGAAGATCTTCTACGACCGCAGCCAGGCCCGCGGCCGGCCGTTCTACTACTTCGCCTTTGGCGCTGCCTGCGTGGAAGTGATCGTCGATACGCTGACCGGCGAATACAA > NC_002947/4869944‑4870229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |