| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,025,416 | C→T | 10.2% | P397L (CCA→CTA) ‡ | PP_4429 → | GntR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,025,416 | 0 | C | T | 10.2% | 128.7 / 5.3 | 49 | P397L (CCA→CTA) ‡ | PP_4429 | GntR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (21/23); new base T (3/2); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
GGTGGATTATGGATGGGAGGGCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGAGTGAAAGCGAGCTA > NC_002947/5025272‑5025564 | ggTGGATTATGGATGGGAGGGCCGACACATTGATCCAATTGCAGCGAAGGCCACCACATCCACGCCAGAAGCGATTGGCAGAGGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGcac > 1:478429/1‑150 (MQ=255) gatggGAGGGCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGtt > 1:306596/1‑150 (MQ=255) tggGAGGGCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTc < 1:1040999/150‑1 (MQ=255) tggGAGGGCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTc < 1:1040840/150‑1 (MQ=255) aGGGCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGc < 1:1173665/150‑1 (MQ=255) aTTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGcac > 2:46530/1‑150 (MQ=255) tCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTc > 1:922900/1‑130 (MQ=255) tCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTc < 2:922900/130‑1 (MQ=255) cAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGa > 1:373241/1‑150 (MQ=255) aaTTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGac > 2:1097848/1‑150 (MQ=255) gCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCa < 2:752913/150‑1 (MQ=255) tCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCGCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCgg > 1:616337/1‑150 (MQ=255) cTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGtt > 1:1161799/1‑150 (MQ=255) agagACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTAc < 2:373241/150‑1 (MQ=255) gAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTg < 2:939483/103‑1 (MQ=255) gAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTg > 1:939483/1‑103 (MQ=255) gCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGtt > 1:524266/1‑150 (MQ=255) gTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCAcc > 1:250160/1‑150 (MQ=255) aGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGCTCACCGTTCg > 2:215573/1‑150 (MQ=255) gACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCgg < 1:334239/150‑1 (MQ=255) aCGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCggg < 2:250160/150‑1 (MQ=255) gaTGGGGCGCTACCGCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGt < 2:616337/150‑1 (MQ=255) ggggCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGAc > 2:411911/1‑150 (MQ=255) gggCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAACATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGAcc < 2:478429/150‑1 (MQ=255) cgcTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCa < 1:529492/150‑1 (MQ=255) tACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCa > 2:209268/1‑150 (MQ=255) ctctATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCAcgc < 1:46530/150‑1 (MQ=255) tctATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCAcgcg < 2:721055/150‑1 (MQ=255) tATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTc < 1:722476/62‑1 (MQ=255) tATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTc > 2:722476/1‑62 (MQ=255) cATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCgg < 2:306596/150‑1 (MQ=255) cccTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTGCGTCACCGGAGCCGTTCACCGTTCgggg < 1:954217/120‑1 (MQ=255) cccTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTg > 2:160535/1‑150 (MQ=255) cccTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCAAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTGCGTCACCGGAGCCGTTCACCGTTCgggg > 2:954217/1‑120 (MQ=255) ccTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGc < 1:209268/150‑1 (MQ=255) tCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCa > 1:667617/1‑127 (MQ=255) tCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCa < 2:667617/127‑1 (MQ=255) tCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGgc > 1:456219/1‑150 (MQ=255) gTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGgcgc > 2:608324/1‑150 (MQ=255) cTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGa < 2:1279018/150‑1 (MQ=255) tcccaaTGTGCTGGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGtt < 2:1070665/83‑1 (MQ=255) tcccaaTGTGCTGGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGtt > 1:1070665/5‑87 (MQ=255) tcccaaTGTGCTGGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGATTTGAATCGCAGCTGAAACGAggca > 2:1036089/5‑147 (MQ=255) cccaaTGTGCTGGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTg > 2:451593/4‑128 (MQ=255) cccaaTGTGCTGGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTg < 1:451593/125‑1 (MQ=255) gAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGCTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGAGTGAAAg < 1:215573/150‑1 (MQ=255) aaTGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGAGTGAAAGc > 2:391576/1‑150 (MQ=255) gtgCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGAGTGAAAGCGAg < 2:1161799/150‑1 (MQ=255) gtgCCAGCACATGGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGAGTGAAAGCGAg < 2:524266/150‑1 (MQ=255) ccAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGAGTGAAAGCGAGCTa < 1:391576/150‑1 (MQ=255) | GGTGGATTATGGATGGGAGGGCCGACACATTGATCCAATTGCAGCGAAGGCTCCTAGAGACACGCCAGAAGCTGGTAGCAGACGAGATGGGGCGCTACCTCTATTGCCATCGCCCTCATTCGTTAAACGCTTGGCTGAATGTGCCAGCACATTGGGATGTTTCCAGCCTGGTGCGCACACTACGACAACAGCATATTGCGGTTACGTCACCGGAGCCGTTCACCGTTCGGGGGGTGACCCCAGCACGCGGCATCCGGCTTTGCATCGGCGCTGAAACGAGTGAAAGCGAGCTA > NC_002947/5025272‑5025564 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |