Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,344,519 C→T 13.9% intergenic (+46/‑132) pgi‑II → / → acsA‑II glucose 6‑phosphate isomerase/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,344,5190CT13.9% 108.7 / 8.4 49intergenic (+46/‑132)pgi‑II/acsA‑IIglucose 6‑phosphate isomerase/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (18/24);  new base T (4/3);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

AGGGCGTTTACCAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATAT  >  NC_002947/5344373‑5344661
                                                                                                                                                  |                                                                                                                                              
aggGCGTTTACCAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAAc                                                                                                                                             <  2:114973/150‑1 (MQ=255)
    cGTTTACCAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGt                                                                                                                                         <  2:663927/150‑1 (MQ=255)
                           gtgtGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGAGCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAcc                                                                                                                  <  1:1089984/147‑1 (MQ=255)
                           gCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAcc                                                                                                                  <  2:154156/150‑1 (MQ=255)
                            ccTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAccc                                                                                                                 <  1:712389/150‑1 (MQ=255)
                             cTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCcg                                                                                                                >  1:113761/1‑150 (MQ=255)
                             cTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCcg                                                                                                                >  1:756324/1‑150 (MQ=255)
                               ggAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGGCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGGAGGAGCGGGTTTACCcgcg                                                                                                              <  2:258296/150‑1 (MQ=255)
                               ggAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCcgcg                                                                                                              <  2:812651/150‑1 (MQ=255)
                                 aaGCCAGCGGCGAACACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCAGAACGTGCCGCTGGAGGTTTCTGGTGCAGGCCTCGTCGAGGGCAACCCCTCCTAGCTGGTAACGCGTGACCTCGTAGGAGCGGGTTTACCCGCGaa                                                                                                            <  1:1254191/150‑1 (MQ=255)
                                 aaGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGaa                                                                                                            <  2:199595/150‑1 (MQ=255)
                                 aaGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGaa                                                                                                            >  1:783203/1‑150 (MQ=255)
                                      aGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAggc                                                                                                       <  1:690851/150‑1 (MQ=255)
                                      aGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCACTTCGCGGGTAAACCCGCCTACAAGCCAACGCGTTACCTCGTAGGAGCGGGGTTACCCGCGCAGAggc                                                                                                       >  1:48899/1‑150 (MQ=39)
                                        cgcCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAggccg                                                                                                     <  1:812911/150‑1 (MQ=255)
                                         gcCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAggccgg                                                                                                    <  2:756324/150‑1 (MQ=255)
                                             aaGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAc                                                                                                                   >  1:171890/1‑131 (MQ=255)
                                             aaGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAc                                                                                                                   <  2:171890/131‑1 (MQ=255)
                                                agggTTCCTCCCAAGGCCGTACCAACTACTTCCGCGGCCGTCCACGCGGTTGAGCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGt                                                                                             <  1:462505/148‑1 (MQ=37)
                                                 cGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGtt                                                                                            <  1:1172243/150‑1 (MQ=255)
                                                      ccACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAaca                                                                                       <  2:113761/150‑1 (MQ=255)
                                                               gCCTGATCACCTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCa                                                                              >  2:1275435/1‑150 (MQ=255)
                                                                ccTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGt                                                                                                                       >  1:296109/1‑108 (MQ=255)
                                                                ccTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGt                                                                                                                       <  2:296109/108‑1 (MQ=255)
                                                                            ctTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAaga                                                                                                          <  2:848499/109‑1 (MQ=255)
                                                                            ctTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAaga                                                                                                          >  1:848499/1‑109 (MQ=255)
                                                                                           ccGCGGTTGATCCGAGATTGTGCCGGCCTCTTCGCGAGTAAAGCCGCGTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGACGAGGCCGGCTCAGGTTGAACACCGCTCCCAGGTACACTGTCTTATCGAATAGCCGTTg                                                  >  2:528226/1‑150 (MQ=38)
                                                                                               ggTTGATCCGAGCTTGGGCCGGCCTCGGCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGt                                              <  2:608446/150‑1 (MQ=255)
                                                                                                        gAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGccc                                     >  1:131927/1‑150 (MQ=255)
                                                                                                               tgCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTg                                                  >  2:1282752/1‑130 (MQ=255)
                                                                                                               tgCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGCTg                                                  <  1:1282752/130‑1 (MQ=255)
                                                                                                                 ccGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAaga                            >  1:551590/1‑150 (MQ=255)
                                                                                                                  cGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAagag                           >  2:949540/1‑150 (MQ=255)
                                                                                                                   ggCTTCTTCGCGGGTAAACCCGCCTACAAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTGGCAGTCCATCGCACCTGACTACAGc                          <  2:48899/150‑1 (MQ=37)
                                                                                                                   ggCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGc                          <  1:949540/150‑1 (MQ=255)
                                                                                                                    gCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCa                         >  2:1025171/1‑150 (MQ=255)
                                                                                                                      ctctTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAgg                       <  2:783203/150‑1 (MQ=255)
                                                                                                                       tctTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGa                      <  1:1275435/150‑1 (MQ=255)
                                                                                                                         ttCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCACACCGCTACACTGTCTTGTCGAGTAACAGTGTCAGTCCATCGCCCCTTACAACAGCAGGacc                    >  2:919520/1‑150 (MQ=38)
                                                                                                                                    ccgctccTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAaga                            <  1:154942/124‑1 (MQ=21)
                                                                                                                                    ccgctccTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAaga                            >  2:154942/6‑129 (MQ=21)
                                                                                                                                    ccgctccTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTc       >  2:963855/6‑150 (MQ=25)
                                                                                                                                     cgctccTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATc                                                              >  2:303284/5‑94 (MQ=14)
                                                                                                                                     cgctccTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATc                                                              <  1:303284/90‑1 (MQ=14)
                                                                                                                                     cccGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCACAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGtt        >  1:968687/1‑150 (MQ=255)
                                                                                                                                      ccgccgACGAGTCAACGCGTTACCTCGTAGGATGTGGTTTACCCGGCAAGAGGCTGGCCCAGGTTGAACACCGCTCCTAGCTACACTGTCTTATTGAATCGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTc       <  2:81181/150‑1 (MQ=255)
                                                                                                                                       cGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCg      >  2:950678/1‑150 (MQ=255)
                                                                                                                                           tACGAGGTAACGCGTTGCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat  >  2:987890/1‑150 (MQ=39)
                                                                                                                                           tACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGatat  >  1:154410/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
AGGGCGTTTACCAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATAT  >  NC_002947/5344373‑5344661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑