Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,406,666 T→C 8.5% R267G (AGG→GGG)  PP_4751 ← amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,406,6660TC 8.5% 133.2 / 2.2 47R267G (AGG→GGG) PP_4751amino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (25/18);  new base C (2/2);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

ACCCTGGACATGCGCAGGGCACAGATGGGAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTGAGTCACGACA  >  NC_002947/5406534‑5406814
                                                                                                                                    |                                                                                                                                                    
aCCCTGGACATGCGCAGGGCACAGATGGGAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACt                                                                                                                                     >  1:867470/1‑150 (MQ=255)
  ccTGGACATGCGCAGGGCACAGATGGGAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTgg                                                                                                                                   >  1:830548/1‑150 (MQ=255)
   cTGGACATGCGCAGGGCACAGATGGGAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGa                                                                                                                                  >  1:787010/1‑150 (MQ=255)
      gACATGCGCAGGGCACAGATGGGAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCa                                                                                                                               >  1:108354/1‑150 (MQ=255)
           gcgcAGGGCACAGATTGGAAAACCCAGCGCCACGCCGACACAGAATGCCAGCACCGTACCGGCAATGGTCCAGGTTATACCGTTCAACAGCATCATCAGGACGTCTTTCATTATCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGt                                                                                                                          <  1:989504/150‑1 (MQ=255)
                   cacaGATGGGAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTc                                                                                                                  <  2:535961/150‑1 (MQ=255)
                     caGATGGGAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCgg                                                                                                                <  1:727594/150‑1 (MQ=255)
                         tGGGAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCCCACTGCCTGAAGGAACt                                                                                                                                     <  1:145595/125‑1 (MQ=255)
                         tGGGAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCCCACTGCCTGAAGGAACt                                                                                                                                     >  2:145595/1‑125 (MQ=255)
                           ggAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTcgc                                                                                                          <  2:830548/150‑1 (MQ=255)
                             aaaCCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCgcgc                                                                                                        >  1:1104386/1‑150 (MQ=255)
                                   cAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACt                                                                                                  >  2:891834/1‑150 (MQ=255)
                                    aGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTc                                                                                                 <  1:891834/150‑1 (MQ=255)
                                                  acaGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCgcgc                                                                                                        <  1:576574/129‑1 (MQ=255)
                                                  acaGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCgcgc                                                                                                        >  2:576574/1‑129 (MQ=255)
                                                  acaGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCg                                                                                   >  2:382016/1‑150 (MQ=255)
                                                    aGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCgtg                                                                                 >  1:118308/1‑150 (MQ=255)
                                                      aaTGCGAGCAAGGTACAGGACGTGGTACCGCGGATTGCGATCAACGGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGtt                                                                               <  2:1104386/150‑1 (MQ=255)
                                                           gAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCgatg                                                                          <  2:108354/150‑1 (MQ=255)
                                                           gAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCgatg                                                                          <  1:382016/150‑1 (MQ=255)
                                                                cGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATgcg                                                                     >  1:623890/1‑150 (MQ=255)
                                                                       ggCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCa                                                              >  1:778994/1‑150 (MQ=255)
                                                                                  aGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGaccac                                                   <  2:972713/150‑1 (MQ=255)
                                                                                   gggTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCa                                                                                                                       >  1:1192999/1‑81 (MQ=255)
                                                                                   gggTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCa                                                                                                                       <  2:1192999/81‑1 (MQ=255)
                                                                                          ccGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATACGCCCGTCAGCCATTACCACCACCCggg                                           >  2:710118/1‑149 (MQ=255)
                                                                                           cGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTc                                          <  2:867470/150‑1 (MQ=255)
                                                                                           cGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTc                                          >  2:530182/1‑150 (MQ=255)
                                                                                                 aCAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGacca                                                    >  1:765348/1‑134 (MQ=255)
                                                                                                 aCAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGacca                                                    <  2:765348/134‑1 (MQ=255)
                                                                                                 aCAGCATCATCAGGATGTATTTCATCAGCGATTACTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGAATCAATCGATGGCTCAACACCTGTTCGATGCTGAGCACGTCAGCCATGACCACCACCCGGTCGGacac                                    >  2:264642/1‑150 (MQ=255)
                                                                                                  taGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGAGGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACg                                   <  2:109211/149‑1 (MQ=255)
                                                                                                      atcatcAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGttt                               >  1:562412/1‑150 (MQ=255)
                                                                                                       tcatcaGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTc                              >  1:1142626/1‑150 (MQ=255)
                                                                                                                   cTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCAt                  <  1:530182/150‑1 (MQ=255)
                                                                                                                    tttCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCAtc                 >  1:824655/1‑150 (MQ=255)
                                                                                                                      tcatcaGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCAtctc               >  2:494838/1‑150 (MQ=255)
                                                                                                                         tcaGCGATTCCTCACTGCCTGAAGGAACTGGATCGGTCTTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTg            >  1:1066913/1‑150 (MQ=255)
                                                                                                                         tcaGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTg            >  1:639433/1‑150 (MQ=255)
                                                                                                                         tcaGCGATTCCCCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTg            >  2:1048490/1‑150 (MQ=255)
                                                                                                                          caGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTGa           <  2:639433/150‑1 (MQ=255)
                                                                                                                          caGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTGa           <  1:710118/150‑1 (MQ=255)
                                                                                                                           aGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTGAg          >  2:172480/1‑150 (MQ=255)
                                                                                                                            gCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTGAGt         >  2:745134/1‑150 (MQ=255)
                                                                                                                              gATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGCGCTCGACCATCCATAGAAAACAAAGAGCCTACCCTCCCTCGTCAACGTCAGTCATGACTACCACCCGGGCGTACACGGTTTCGGCGAAGTGCATCTCGTGAGTCa       <  2:787010/150‑1 (MQ=255)
                                                                                                                               aTTCCCCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTGAGTCAc      <  1:1048490/150‑1 (MQ=255)
                                                                                                                                   cTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTGAGTCACGACa  >  1:587413/1‑150 (MQ=255)
                                                                                                                                    |                                                                                                                                                    
ACCCTGGACATGCGCAGGGCACAGATGGGAAACCCCAGCACTACGCCGACACAGAATGCGAGCACGGTAACGGCAATGGTCCAGGGTATGCCGTTCAACAGCATCATCAGGATGTCTTTCATCAGCGATTCCTCACTGCCTGAAGGAACTGGATCACCCGTTCATGCTCGGGCTCGCGCATCACTCGCTGGCTCGGCCCGTGTTCGATGATGCGCCCGTCAGCCATGACCACCACCCGGTCGGACACGGTTTCGGCGAAGTGCATCTCGTGAGTCACGACA  >  NC_002947/5406534‑5406814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑