Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 36,683 C→T 9.6% A324T (GCG→ACG)  PP_0033 ← undecaprenyl‑glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294736,6830CT 9.6% 138.9 / 5.3 52A324T (GCG→ACG) PP_0033undecaprenyl‑glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (22/25);  new base T (2/3);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

ATTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAACAAGGCCCATGG  >  NC_002947/36534‑36824
                                                                                                                                                     |                                                                                                                                             
aTTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAgcgc                                                                                                                                               <  2:745874/150‑1 (MQ=255)
  tCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCACACTTTTCTGACCCGTGCAGACAGCAGAAATGCCCACGCTGGCAGCGCgg                                                                                                                                             <  1:1258796/150‑1 (MQ=255)
   atctgggCGCTCTTCCAGGACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCggg                                                                                                                                            <  2:873983/146‑1 (MQ=255)
     gAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTa                                                                                                                                          <  1:995930/150‑1 (MQ=255)
      aGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCGCGCTGCCAGCGTgggcat                                                                                                                                         <  1:51318/150‑4 (MQ=255)
        ggCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGGGACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAgat                                                                                                                                       >  1:873983/1‑148 (MQ=255)
               ctctAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCaggtaggt                                                                                                                                >  1:425762/1‑150 (MQ=255)
                           ggCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGtt                                                                                                                    <  1:745341/150‑1 (MQ=255)
                             cGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGc                                                                                                                  <  1:1004812/150‑1 (MQ=255)
                             cGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGc                                                                                                                  <  1:549450/150‑1 (MQ=255)
                               tAGATGATGCCCGCACCGATGATTGGCAACACCTATGCTCGCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCt                                                                                                                <  2:425762/150‑1 (MQ=255)
                               tAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCt                                                                                                                <  2:709349/150‑1 (MQ=255)
                                 gatgatGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGc                                                                                                              >  2:483794/1‑150 (MQ=255)
                                     atgCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAt                                                                                                          <  1:1167794/150‑1 (MQ=255)
                                       gCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtgt                                                                                                        <  2:1087561/150‑1 (MQ=255)
                                         ccGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTga                                                                                                      >  1:61223/1‑150 (MQ=255)
                                          cGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTgaa                                                                                                     >  2:1285087/1‑150 (MQ=255)
                                                 atgatTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGAcc                                                                                              <  2:1005756/150‑1 (MQ=255)
                                                            cacCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCaa                                                                                   <  1:146736/150‑1 (MQ=255)
                                                             acCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGcgcg                                                                                                                                              >  1:2980/1‑90 (MQ=255)
                                                             acCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGcgcg                                                                                                                                              <  2:2980/90‑1 (MQ=255)
                                                               cAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTCCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGc                                                                                >  1:1096552/1‑150 (MQ=255)
                                                               cAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGACAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACACAGCATTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGc                                                                                >  1:337330/1‑150 (MQ=255)
                                                                         gCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTga                                                                                                      <  1:4100/118‑1 (MQ=255)
                                                                         gCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTga                                                                                                      >  2:4100/1‑118 (MQ=255)
                                                                         gCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCag                                                                      >  1:815792/1‑150 (MQ=255)
                                                                          cAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCaga                                                                     <  2:127678/150‑1 (MQ=255)
                                                                                   cccAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCtt                                                            >  1:600760/1‑150 (MQ=255)
                                                                                    ccAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTa                                                                 >  2:560319/1‑144 (MQ=255)
                                                                                    ccAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTa                                                                 <  1:560319/144‑1 (MQ=255)
                                                                                    ccAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGACCACAGCCACAGGTAGGCgtt                                                           >  2:397694/1‑150 (MQ=255)
                                                                                     cAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCca                                                                                                                             <  1:737089/83‑1 (MQ=255)
                                                                                     cAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCca                                                                                                                             >  2:737089/1‑83 (MQ=255)
                                                                                           gtCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAgcgc                                                    >  2:628795/1‑150 (MQ=255)
                                                                                              ttCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGc                                                 >  1:1130521/1‑150 (MQ=255)
                                                                                              ttCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGc                                                 >  1:305599/1‑150 (MQ=255)
                                                                                               tCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCa                                                <  1:628795/150‑1 (MQ=255)
                                                                                                   gCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtgt                                                                                                        >  2:1135624/1‑90 (MQ=255)
                                                                                                   gCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtgt                                                                                                        <  1:1135624/90‑1 (MQ=255)
                                                                                                        cTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCAc                                       >  2:1048337/1‑150 (MQ=255)
                                                                                                         ttCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACg                                      >  2:584082/1‑150 (MQ=255)
                                                                                                               gACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCg                                <  1:483794/150‑1 (MQ=255)
                                                                                                               gACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCg                                <  1:397694/150‑1 (MQ=255)
                                                                                                                aCCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCgg                               <  1:1048337/150‑1 (MQ=255)
                                                                                                                       cAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtg                                                                                                         <  1:978153/69‑1 (MQ=255)
                                                                                                                       cAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtg                                                                                                         >  2:978153/1‑69 (MQ=255)
                                                                                                                             gcagcaATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTa                  <  2:305599/150‑1 (MQ=255)
                                                                                                                               accaATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGa                >  2:960793/3‑150 (MQ=255)
                                                                                                                                  aaTGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAAc             <  2:61223/150‑1 (MQ=255)
                                                                                                                                    tGCCCACGCTGGCAGCGCGGGTAGCAAGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAACaa           >  1:261448/1‑150 (MQ=255)
                                                                                                                                     acccgcGCTGCCAGCGTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTg                     >  2:385611/6‑139 (MQ=255)
                                                                                                                                     acccgcGCTGCCAGCGTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTg                     <  1:385611/134‑1 (MQ=255)
                                                                                                                                      cccgcGCTGCCAGCGTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCtt                                   <  2:1245228/120‑1 (MQ=255)
                                                                                                                                      cccGCGCTGCCAGCGTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCtt                                   >  1:1245228/1‑124 (MQ=255)
                                                                                                                                             tGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAACAAGGCCCATgg  <  1:960793/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
ATTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGCGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAACAAGGCCCATGG  >  NC_002947/36534‑36824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑