Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,128,641 A→G 8.5% intergenic (‑701/‑221) PP_5375 ← / → PP_5376 LysR family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,128,6410AG 8.5% 128.7 / 2.2 47intergenic (‑701/‑221)PP_5375/PP_5376LysR family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/25);  new base G (2/2);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

CGAGGCCTCATTTCATGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGAT  >  NC_002947/6128494‑6128789
                                                                                                                                                   |                                                                                                                                                    
cGAGGCCTCATTTCATGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAAt                                                                                                                                                    <  2:813153/150‑1 (MQ=255)
  aGGCCTCATTTCATGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCa                                                                                                                                                  <  2:142624/150‑1 (MQ=255)
    gCCTCATTTCATGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGCTTTGATTCaaaacgt                                                                                                                                                <  2:1014140/150‑6 (MQ=255)
     ccTCATTTCATGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGtt                                                                                                                                               >  2:557583/1‑150 (MQ=255)
     ccTCATTTCATGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGtt                                                                                                                                               <  1:913083/150‑1 (MQ=255)
      cTCATTTCATGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCAAGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTc                                                                                                                                              >  1:519317/1‑150 (MQ=255)
        cATTTCATGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTcga                                                                                                                                            <  2:1179597/150‑1 (MQ=255)
               tGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGt                                                                                                                                                <  2:449039/139‑1 (MQ=255)
               tGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGt                                                                                                                                                >  1:449039/1‑139 (MQ=255)
                 gggCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAaccca                                                                                                                                   <  2:306763/150‑1 (MQ=255)
                    ccTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAacccaccc                                                                                                                                <  2:917481/150‑1 (MQ=255)
                        cGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGAt                                                                                                                            >  2:191597/1‑150 (MQ=255)
                           gCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCAc                                                                                                                         <  1:777109/150‑1 (MQ=255)
                                cTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCAATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCa                                                                                                                                                  <  2:8767/120‑1 (MQ=255)
                                cTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCAATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCa                                                                                                                                                  >  1:8767/1‑120 (MQ=255)
                                 ttGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAg                                                                                                                   <  1:193649/150‑1 (MQ=255)
                                  tGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAgg                                                                                                                  <  1:191597/150‑1 (MQ=255)
                                    gagaAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCt                                                                                                                >  2:701545/1‑150 (MQ=255)
                                           tGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATAtt                                                                                                         >  2:120872/1‑150 (MQ=255)
                                              aaaaCTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCa                                                                                                      >  1:380090/1‑150 (MQ=255)
                                                aaCTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAgg                                                                                                    >  1:109443/1‑150 (MQ=255)
                                                   tGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGttt                                                                                                 >  1:545549/1‑150 (MQ=255)
                                                     tCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGc                                                                                               <  1:120872/150‑1 (MQ=255)
                                                       gCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCct                                                                                             >  1:1207324/1‑150 (MQ=255)
                                                         aaaaGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGGATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCctct                                                                                           >  1:129573/1‑150 (MQ=255)
                                                         aaaaGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGGATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCctct                                                                                           >  1:129616/1‑150 (MQ=255)
                                                             gCACGAAATACCGCTTCAGGCGATCGCGTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGc                                                                                       >  1:1265268/1‑150 (MQ=255)
                                                              cACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATAACGCGGCTTTGCGGTGGATTTGATTAAATCAGTTATAAGAAACCCACCCGGAACACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGca                                                                                      >  2:34883/1‑150 (MQ=255)
                                                               aCGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGcaa                                                                                     <  2:380090/150‑1 (MQ=255)
                                                                    aTACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATc                                                                                <  2:109443/150‑1 (MQ=255)
                                                                         gCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCt                                                                           <  2:519317/150‑1 (MQ=255)
                                                                          cTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTa                                                                          <  2:545549/150‑1 (MQ=255)
                                                                           ttCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTg                                                                                                               >  1:203405/1‑112 (MQ=255)
                                                                           ttCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTg                                                                                                               <  2:203405/112‑1 (MQ=255)
                                                                                        tttAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCa                                                                       <  2:753606/139‑1 (MQ=255)
                                                                                        tttAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCa                                                                       >  1:753606/1‑139 (MQ=255)
                                                                                              gaATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCAATTa                                                      <  1:701545/150‑1 (MQ=255)
                                                                                               aaTTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAg                                                     >  1:950033/1‑150 (MQ=255)
                                                                                                aTTAAAGCTGTCGCCGCGAGTAATGACGCGGCGTTGTGGGGGATTTGATGGAAGCAGGGCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAgg                                                    <  2:489879/150‑1 (MQ=255)
                                                                                                aTTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAgg                                                    <  1:251811/150‑1 (MQ=255)
                                                                                                 ttAAAACTGTCGCCGCCAGTAATGACGCGGCTTGGCGGTGGAGTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGc                                                   >  1:28617/1‑150 (MQ=255)
                                                                                                   aaaaCTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGGATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCAt                                                 <  2:129616/150‑1 (MQ=255)
                                                                                                   aaaaCTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGGATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCAt                                                 <  2:129573/150‑1 (MQ=255)
                                                                                                                 cattAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGgt                                   <  2:394546/147‑1 (MQ=255)
                                                                                                                 cAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGgt                                   <  1:557583/150‑1 (MQ=255)
                                                                                                                          cgcgGCTTTGCGGTGGATTTGATTGAATCAGTTCGAAGCAACCCGCCCGGATCACTATGAGGCTGGATATTTCAGGGTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCttt                          <  1:85351/150‑1 (MQ=255)
                                                                                                                                           tttGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGa             <  1:103003/146‑1 (MQ=255)
                                                                                                                                           tttGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGa             >  2:103003/1‑146 (MQ=255)
                                                                                                                                            ttgattgaATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCAAGTCGTTAGCTTTGCACCGTCTGCGACTGAc        <  1:34344/150‑1 (MQ=255)
                                                                                                                                             tgattgaATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACa       <  2:1207324/150‑1 (MQ=255)
                                                                                                                                                  aaaTCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGAt  <  2:1265268/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGAGGCCTCATTTCATGGGGCCTGCGGGCTCCCTTGGAGAAAATGCAAAACTGTCGCAAAAGCACGAAATACCGCTTCAGGCGATCGCTTTAGAGAATTAAAACTGTCGCCGCCAGTAATGACGCGGCTTTGCGGTGGATTTGATTGAATCAGTTCGACGAAACCCACCCGGATCACTATGAGGCTGGATATTTCAGGTTTGCCTCTCAGCAACAATCCTTCTAGCAGTATAGGCTGCCCATTAGGCATCACTCTTGCCAGGTGTCAGCTTTGCACCGTCTGCGACTGACAAAGAT  >  NC_002947/6128494‑6128789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑