Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 387,343 G→C 7.8% E160D (GAG→GAC)  glyA‑I → serine hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947387,3430GC 7.8% 155.8 / 5.3 51E160D (GAG→GAC) glyA‑Iserine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base C (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

CCGGTGACACCATCCTGGGCATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAGGTGGGCGCACTGCTGTT  >  NC_002947/387198‑387459
                                                                                                                                                 |                                                                                                                    
ccGGTGACACCATCCTGGGCATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGcc                                                                                                                  <  2:986439/150‑1 (MQ=255)
 cGGTGACACCATCCTGGGCATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGACCGcca                                                                                                                 >  2:407305/1‑149 (MQ=255)
    tGACACCATCCTGGGCATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGc                                                                                                              <  2:173203/150‑1 (MQ=255)
        acCATCCTGGGCATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTc                                                                                                          >  1:22099/1‑150 (MQ=255)
          cATCCTGGGCATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGa                                                                                                        >  1:678327/1‑150 (MQ=255)
            tCCTGGGCATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTGCGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGc                                                                                                      >  1:356244/1‑150 (MQ=255)
                 ggCATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAg                                                                                                 >  2:634438/1‑150 (MQ=255)
                 ggCATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGACCGCCAGGCGCTCGacttcgtc                                                                                                 <  1:407305/150‑8 (MQ=38)
                   cATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGcc                                                                                               >  2:477916/1‑150 (MQ=255)
                       aGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGACCGCCAGGCGCTCGa                                                                                                        <  2:1062363/137‑1 (MQ=255)
                       aGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGACCGCCAGGCGCTCGa                                                                                                        >  1:1062363/1‑137 (MQ=255)
                        gCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGaaaa                                                                                          <  1:480034/150‑1 (MQ=255)
                               ccACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGt                                                                                   >  1:578354/1‑150 (MQ=255)
                                   ggcggTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGcc                                                                               <  2:985899/150‑1 (MQ=255)
                                   ggccgTCGCCTGACCCACTGTGGCAAGGGGGCTGCCTCTGGCAAACTGTACAACGCCGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTCTCTGTCGAGCGCAAGCCCAAAATGCTCGTTGcc                                                                               <  2:82321/150‑1 (MQ=17)
                                      ggTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGt                                                                            <  1:471819/150‑1 (MQ=255)
                                      ggTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGt                                                                            <  2:578354/150‑1 (MQ=255)
                                                     ggCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTcc                                                             <  1:634438/150‑1 (MQ=255)
                                                         ccAAAGTTTCGTCATCGGTCAAACTGTACACCGCACGCCAGTACGGCATCAATACCAACTCCCGCCTTATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCTGCCTCCTCCAAGa                                                         <  2:264537/150‑1 (MQ=21)
                                                          cAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGttt                                                                          <  2:229508/132‑1 (MQ=255)
                                                          cAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGttt                                                                          >  1:229508/1‑132 (MQ=255)
                                                          cAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGAc                                                        >  1:1093802/1‑150 (MQ=255)
                                                            aGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCGGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGAccc                                                      >  2:876051/1‑150 (MQ=255)
                                                              gtgtCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGGTCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTc                                                    <  1:335498/150‑1 (MQ=255)
                                                              gtgtCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTc                                                    >  1:280074/1‑150 (MQ=255)
                                                               tgtCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCg                                                   >  1:334110/1‑150 (MQ=255)
                                                               tgtCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCg                                                   >  1:453426/1‑150 (MQ=255)
                                                                 tcgtaCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGAc                                                 >  2:720510/1‑150 (MQ=255)
                                                                        cGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCAc                                          <  1:876051/150‑1 (MQ=255)
                                                                              aaCTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTcc                                    <  1:55551/150‑1 (MQ=255)
                                                                                cTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCcgc                                  >  1:1206627/1‑150 (MQ=255)
                                                                                cTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTAATCCAAGACCCTCGACTTCCCACGTTTCcgc                                  <  2:678327/150‑1 (MQ=255)
                                                                                 tGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCcgcg                                 >  2:478074/1‑150 (MQ=255)
                                                                                      aaCGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCg                                                                      >  2:914720/1‑108 (MQ=255)
                                                                                      aaCGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCg                                                                      <  1:914720/108‑1 (MQ=255)
                                                                                               cAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATCATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAg                   >  2:1108743/1‑150 (MQ=255)
                                                                                                 gTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAGGt                 <  2:1093802/150‑1 (MQ=255)
                                                                                                     ggCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAGGTGGgc             >  2:113037/1‑150 (MQ=255)
                                                                                                     ggCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAGGTGGgc             <  2:152746/150‑1 (MQ=255)
                                                                                                       cATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGGCATCGCCGACAAGGTGGgcgc           >  2:659056/1‑150 (MQ=255)
                                                                                                         tCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCATGGCGGTCGAGCACAACCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAAGTGGGCGCAc         <  1:139894/150‑1 (MQ=255)
                                                                                                           gACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAACATGATCGTCGACGGTTTCTCGGCTGGTGCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAGGTGGGCGCActg       <  1:478074/150‑1 (MQ=25)
                                                                                                            acacCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAGGTGGGCGCActgc      >  2:506929/1‑150 (MQ=255)
                                                                                                            acacCAACACCGGACTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAGGTGGGCGCActgc      <  1:28079/150‑1 (MQ=255)
                                                                                                                cAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAGGTGGGCGCACTGCTGtt  >  2:340002/1‑150 (MQ=255)
                                                                                                                    acCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGa                                                  >  2:475646/1‑98 (MQ=255)
                                                                                                                    acCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGa                                                  <  1:475646/98‑1 (MQ=255)
                                                                                                                      cGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGa                                                         >  1:814127/1‑89 (MQ=255)
                                                                                                                      cGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGa                                                         <  2:814127/89‑1 (MQ=255)
                                                                                                                              tCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTccc                                            >  2:19091/1‑94 (MQ=255)
                                                                                                                              tCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTccc                                            <  1:19091/94‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                    
CCGGTGACACCATCCTGGGCATGAGCCTGGCCCACGGCGGTCACCTGACCCACGGCGCCAAGGTGTCGTCCTCGGGCAAACTGTACAACGCAGTCCAGTACGGCATCGACACCAACACCGGCCTGATCGACTACGACGAAGTCGAGCGCCTGGCGGTCGAGCACAAGCCGAAAATGATCGTTGCCGGTTTCTCGGCCTACTCCAAGACCCTCGACTTCCCACGTTTCCGCGCCATCGCCGACAAGGTGGGCGCACTGCTGTT  >  NC_002947/387198‑387459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑