Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,762	C→G	S269W (TCG→TGG)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,762	0	C	G	100.0%	204.3 / NA	60	S269W (TCG→TGG)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (30/30);  total (30/30)

AGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGG  >  NC_002947/1851618‑1851905                                                                                                                                                 |                                                                                                                                                 aGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCt                                                                                                                                             >  2:139852/1‑150 (MQ=255)   tACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTgg                                                                                                                                           >  1:347672/1‑150 (MQ=255)           gATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGAt                                                                                                                                   <  1:211800/150‑1 (MQ=255)            aTCGCCAAGCTGTTGCATGAGCGGCGGCTGGACAGCGAGCGCTTCCTCTGCGTCGTGATGAACCAGCGGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATg                                                                                                                                  <  2:347672/150‑1 (MQ=255)                ccAAGCTGTTGCATGAGCGGCGGCTGGACCGCGGGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAgc                                                                                                                              >  2:60135/1‑150 (MQ=255)                     cTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCaaa                                                                                                                         >  1:559628/1‑150 (MQ=255)                          gCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCt                                                                                                                    >  2:1141156/1‑150 (MQ=255)                           cATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTg                                                                                                                   >  1:1161325/1‑150 (MQ=255)                             tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATGTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                 <  2:598978/150‑1 (MQ=255)                                             cgcgAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTac                                                                                                 <  2:804305/150‑1 (MQ=255)                                                gAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTacgac                                                                                              >  2:1210128/1‑150 (MQ=255)                                                gAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTacgac                                                                                              <  1:1141156/150‑1 (MQ=255)                                                 aGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTacgac‑g                                                                                            <  1:60135/150‑1 (MQ=255)                                                  gcgcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                 <  2:363821/129‑1 (MQ=255)                                                  gcgcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                 >  1:363821/1‑129 (MQ=255)                                                      ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCCCTGTCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCt                                                                                                   <  1:1055275/139‑1 (MQ=255)                                                      ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTg                                                                                                                   <  2:864051/123‑1 (MQ=255)                                                      ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTg                                                                                                                   >  1:864051/1‑123 (MQ=255)                                                      ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCt                                                                                                   >  2:1055275/1‑139 (MQ=255)                                                        catcaGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑gtgcgtgc                                                                                     >  1:796027/1‑150 (MQ=255)                                                         atccGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑Gtgcgtgcg                                                                                    >  2:1044147/1‑150 (MQ=255)                                                             gCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAACGGCCTGGAGTTCAGCCAGATCTACGAC‑CTGCgtgcggtgc                                                                                >  2:1584487/1‑150 (MQ=255)                                                                    gatgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGgcgc                                                                                                                                        <  1:1475277/88‑1 (MQ=255)                                                                    gatgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGgcgc                                                                                                                                        >  2:1475277/1‑88 (MQ=255)                                                                     atgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTg                                                                        <  2:1437248/150‑1 (MQ=255)                                                                     atgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGACAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTg                                                                        <  2:1021556/150‑1 (MQ=255)                                                                      tgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTgg                                                                       >  2:518241/1‑150 (MQ=255)                                                                          ccAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                 <  1:541273/105‑1 (MQ=255)                                                                          ccAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                 >  2:541273/1‑105 (MQ=255)                                                                          ccAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTac                                                                                                 <  1:354/121‑1 (MQ=255)                                                                          ccAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTac                                                                                                 >  2:354/1‑121 (MQ=255)                                                                           cAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCg                                                                  >  1:1116856/1‑150 (MQ=255)                                                                                 cAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATc                                                            <  1:1210128/150‑1 (MQ=255)                                                                                  aGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATcc                                                           >  1:905007/1‑150 (MQ=255)                                                                                   gAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTCTTGGCTCTGGACCAAGCAGCAGCGCAAAGGCCTGGCGTTGAAGTCGTTGCAAAACAGGGCGTG‑GGTGCGCGTGCTGGTGCCGAAAATccc                                                          >  1:354965/1‑149 (MQ=255)                                                                                    aaCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcgc                                                         >  2:253785/1‑150 (MQ=255)                                                                                    aaCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcgc                                                         >  2:1496386/1‑150 (MQ=255)                                                                                              tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAc‑gtgc                                                                                         <  2:948658/108‑1 (MQ=255)                                                                                              tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAc‑gtgc                                                                                         >  1:948658/1‑108 (MQ=255)                                                                                               cGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTacac                                              <  2:252717/150‑1 (MQ=255)                                                                                                      ggCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTg                                       <  2:559628/150‑1 (MQ=255)                                                                                                       gCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTgg                                      <  1:637496/150‑1 (MQ=255)                                                                                                         gTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTCAGGTGCTCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGc                                    <  1:1231227/150‑1 (MQ=255)                                                                                                              ggCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTa                                                                                                  <  2:183623/84‑1 (MQ=255)                                                                                                              ggCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTa                                                                                                  >  1:183623/1‑84 (MQ=255)                                                                                                                ccGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGc                             >  2:286779/1‑150 (MQ=255)                                                                                                                 cGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGca                            <  1:518241/150‑1 (MQ=255)                                                                                                                         gCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTtgt                    <  2:1116856/150‑1 (MQ=255)                                                                                                                              cGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTggcgg               <  2:1161325/150‑1 (MQ=255)                                                                                                                               gggCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGa                                                       >  1:334651/1‑109 (MQ=255)                                                                                                                               gggCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGa                                                       <  2:334651/109‑1 (MQ=255)                                                                                                                                        atatCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGca                            <  2:717083/127‑1 (MQ=255)                                                                                                                                        atatCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGca                            >  1:717083/1‑127 (MQ=255)                                                                                                                                        atatCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCa     >  1:1517908/1‑150 (MQ=255)                                                                                                                                         tatCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCaa    <  1:286779/150‑1 (MQ=255)                                                                                                                                         tatCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCaa    <  2:56972/150‑1 (MQ=255)                                                                                                                                         tatCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCaa    <  1:1496386/150‑1 (MQ=255)                                                                                                                                           tCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAgg  >  2:754650/1‑150 (MQ=255)                                                                                                                                             tATTGGATCTGGCGCAAGATGCGGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCgtgcggt                                                                                  >  1:257971/1‑68 (MQ=255)                                                                                                                                             tATTGGATCTGGCGCAAGATGCGGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCgtgcggt                                                                                  <  2:257971/68‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 AGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC‑GTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGG  >  NC_002947/1851618‑1851905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑