Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,656,409	C→T	R446H (CGC→CAC)	tktA ←	transketolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,656,409	0	C	T	100.0%	219.1 / NA	66	R446H (CGC→CAC)	tktA	transketolase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/34);  total (32/34)

GCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTGGCGTC  >  NC_002947/5656265‑5656551                                                                                                                                                 |                                                                                                                                               gCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACg                                                                                                                                           >  2:1022555/1‑150 (MQ=255)      gcgcCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATAGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGtt                                                                                                                                      <  2:291534/150‑1 (MQ=255)       cgcCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTg                                                                                                                                     <  1:1333910/150‑1 (MQ=255)          cAGGTGTCCAGGTTCGGGGTGCTGCCGGGACTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCgg                                                                                                                                  >  1:1082593/1‑150 (MQ=255)           aGGTGTCCAGGTTCGGGGTGCTGCGTAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCggg                                                                                                                                 <  1:1007478/150‑1 (MQ=255)                tCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTGCGCCCAGACCAATGGAGTCGTGGGTGTACACGTGGGTCACGCGCGGCTTCATCAGGGCAGACAGGTGCACGGCGTTGCGGGCGTAt                                                                                                                            >  2:588356/1‑150 (MQ=255)                 ccAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTAtt                                                                                                                           >  2:1213124/1‑150 (MQ=255)                  gaGGTTCGGGGTGCTGCGAAGGCTGGTCAGCTGCTGGGTCGGGTGGTGCGTCGGGCCGTCTTCTCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGGACGGCGTTGCTGGCGTCTTc                                                                                                                          >  2:557847/2‑150 (MQ=255)                  cAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTc                                                                                                                          >  2:635676/1‑150 (MQ=255)                    ggTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCa                                                                                                                        <  2:662958/150‑1 (MQ=255)                     gTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGCTCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCAt                                                                                                                       <  1:598714/150‑1 (MQ=255)                      ttCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGTGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATg                                                                                                                      <  2:1082593/150‑1 (MQ=255)                            gTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATc                                                                                                                <  1:509116/150‑1 (MQ=255)                             tgctgcGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATc                                                                                                                <  2:72932/149‑1 (MQ=255)                             tgctgcGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATc                                                                                                                >  1:72932/1‑149 (MQ=255)                             tgctgcGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCa                                                                                                               <  1:474064/150‑1 (MQ=255)                               ctgcGCAGGCTGGTCAGCTGCTGGCTCGGCTGATTCGTCGGGCCGTCTGCGCCCAGACCGATTGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAgg                                                                                                             <  2:417250/150‑1 (MQ=255)                               ctgcGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAgg                                                                                                             <  1:1022555/150‑1 (MQ=255)                                tgcGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCCCGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGa                                                                                                            <  2:1270504/150‑1 (MQ=255)                                       gCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTcgc                                                                                                     >  1:1078855/1‑150 (MQ=255)                                          ggTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGcc                                                                                                  <  1:635676/150‑1 (MQ=255)                                              aGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTaa                                                                                              <  1:1213124/150‑1 (MQ=255)                                                ctgctCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACAAACGCCACTCGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAgg                                                                                            <  1:1287687/150‑1 (MQ=255)                                                 tgcgCGATCGGGTGGTGCGTCGGGCCGTCTTCGCCCAGCCCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGc                                                                                           <  2:730166/150‑1 (MQ=255)                                                       aTCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAgg                                                                                     <  2:997276/150‑1 (MQ=255)                                                        tCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGc                                                                                    <  2:1078855/150‑1 (MQ=255)                                                             tGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCg                                                                               >  1:1114256/1‑150 (MQ=255)                                                                   gTCGGGCCGCCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAgg                                                                         >  2:147715/1‑150 (MQ=255)                                                                            tCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCg                                                                                                                                        <  2:1444149/78‑1 (MQ=255)                                                                            tCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCg                                                                                                                                        >  1:1444149/1‑78 (MQ=255)                                                                            tCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTc                                                                                                       <  2:1274008/111‑1 (MQ=255)                                                                            tCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTc                                                                                                       >  1:1274008/1‑111 (MQ=255)                                                                             cTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGt                                                               <  1:147715/150‑1 (MQ=255)                                                                                cGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCa                                                            <  1:554574/150‑1 (MQ=255)                                                                                  cccAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCa                                                                                                                        >  1:243523/1‑88 (MQ=255)                                                                                  cccAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCa                                                                                                                        <  2:243523/88‑1 (MQ=255)                                                                                          gATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATgg                                                      <  2:39336/146‑1 (MQ=255)                                                                                          gATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATgg                                                      >  1:39336/1‑146 (MQ=255)                                                                                          gATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGt                                                  >  2:1067871/1‑150 (MQ=255)                                                                                            tGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCa                                                <  2:150190/150‑1 (MQ=255)                                                                                              gAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCgcgc                                                                                                   >  2:1242795/1‑97 (MQ=255)                                                                                              gAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCgcgc                                                                                                   <  1:1242795/97‑1 (MQ=255)                                                                                                gTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGAACTTTGCCCGGCGTTGTGTGCCTTTTCCAGGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGcc                                            <  1:588356/150‑1 (MQ=255)                                                                                                   gTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGaa                                         >  2:769662/1‑150 (MQ=255)                                                                                                   gTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGaa                                         >  1:61674/1‑150 (MQ=255)                                                                                                    tGGGTGTACACGGGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAAc                                        >  2:1453988/1‑150 (MQ=255)                                                                                                       gtgtACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTcg                                     >  1:146069/1‑150 (MQ=255)                                                                                                        tgtACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTaa                                                                                              >  2:1213593/1‑92 (MQ=255)                                                                                                        tgtACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTaa                                                                                              <  1:1213593/92‑1 (MQ=255)                                                                                                        tgtACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCatg                                                          <  2:813727/128‑1 (MQ=255)                                                                                                        tgtACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGACACCAGGCCACCGTGCAGGGCAACGCCGTTCatg                                                          >  1:813727/1‑128 (MQ=255)                                                                                                          tACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCgcgc                                  >  1:57348/1‑150 (MQ=255)                                                                                                               gTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCCGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGcc                                                                 <  1:1472898/114‑1 (MQ=255)                                                                                                               gTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCCGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGcc                                                                 >  2:1472898/1‑114 (MQ=255)                                                                                                                  gATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTa                          <  1:769662/150‑1 (MQ=255)                                                                                                                      aCGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAac                      >  1:1222288/1‑150 (MQ=255)                                                                                                                        gcgcTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAacac                    <  2:1114256/150‑1 (MQ=255)                                                                                                                        gcgcTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAacac                    >  2:127119/1‑150 (MQ=255)                                                                                                                                  attggggCCGACATGTGCACGGCGTTGCGGGCGTATTACATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTAGCGCACGCCGTAGAACACGTAGTTGCCg          >  2:426067/5‑150 (MQ=255)                                                                                                                                   tcaGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGt                                                               >  1:274973/1‑96 (MQ=255)                                                                                                                                   tcaGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGt                                                               <  2:274973/96‑1 (MQ=255)                                                                                                                                   tcaGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCATTCatg                                                          <  1:1398565/101‑1 (MQ=255)                                                                                                                                   tcaGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCATTCatg                                                          >  2:1398565/1‑101 (MQ=255)                                                                                                                                      gggCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTgg      >  1:231372/1‑150 (MQ=255)                                                                                                                                      gggCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTgg      >  2:1482433/1‑150 (MQ=255)                                                                                                                                          cGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTGGCGTc  >  2:569433/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                               GCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTGGCGTC  >  NC_002947/5656265‑5656551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑