Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,964,640	G→A	Q255* (CAG→TAG)	fleQ ←	transcriptional regulator FleQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,964,640	0	G	A	100.0%	179.2 / NA	57	Q255* (CAG→TAG)	fleQ	transcriptional regulator FleQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/24);  total (33/24)

ACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGG  >  NC_002947/4964493‑4964784                                                                                                                                                    |                                                                                                                                                 aCAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACa                                                                                                                                                >  1:1337779/1‑150 (MQ=255)            cgGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGc                                                                                                                                     >  1:612602/1‑150 (MQ=255)                       gTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACAGGCAGCGGCTTGACCTACATCGGCAACGGCATGTCGCCGAt                                                                                                                          >  1:198971/1‑150 (MQ=255)                           tCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTtcg                                                                                                                      >  1:1619536/1‑150 (MQ=255)                           tCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTtcg                                                                                                                      >  1:1670082/1‑150 (MQ=255)                            cGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTtcgt                                                                                                                     <  1:1209653/150‑1 (MQ=255)                                     tttCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCg                                                                                                                               >  2:1598538/1‑131 (MQ=255)                                     tttCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCg                                                                                                                               <  1:1598538/131‑1 (MQ=255)                                     tttCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGcc                                                                                                                             <  2:762334/133‑1 (MQ=255)                                     tttCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGcc                                                                                                                             >  1:762334/1‑133 (MQ=255)                                     tttCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACa                                                                                                            <  1:1526321/150‑1 (MQ=255)                                      ttCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAg                                                                                                           <  2:1083303/150‑1 (MQ=255)                                        cGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAggg                                                                                                         >  2:1176013/1‑150 (MQ=255)                                         gAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGAAACTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGt                                                                                                        <  1:597653/150‑1 (MQ=255)                                          aGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTa                                                                                                       <  2:612602/150‑1 (MQ=255)                                             ttCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTAccg                                                                                                    >  1:1216404/1‑150 (MQ=255)                                                     cGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGc                                                                                            <  2:645572/150‑1 (MQ=255)                                                               atgatgCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGaaa                                                                                  >  1:282227/1‑150 (MQ=255)                                                                      gcACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCgg                                                                           >  2:943599/1‑150 (MQ=255)                                                                         cGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCTAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAc                                                                        <  2:1337779/150‑1 (MQ=255)                                                                              aTGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTg                                                                   >  1:1788796/1‑150 (MQ=255)                                                                               tGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTgg                                                                  >  1:489410/1‑150 (MQ=255)                                                                                gCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTAAGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGt                                                                 >  1:1496960/1‑150 (MQ=255)                                                                                 cTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTg                                                                >  1:1171508/1‑150 (MQ=255)                                                                                     gggTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATgg                                                            <  2:334797/150‑1 (MQ=255)                                                                                        tCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGccc                                                         <  2:1788796/150‑1 (MQ=255)                                                                                          ttgttgCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCg                                                       <  2:1619536/150‑1 (MQ=255)                                                                                          ttgttgCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCg                                                       >  1:446595/1‑150 (MQ=255)                                                                                            gttgCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGACGCGATCGGCACGGCTGGTGATGGCCCCGGt                                                     >  1:467935/1‑150 (MQ=38)                                                                                             ttgCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTa                                                    >  2:705747/1‑150 (MQ=255)                                                                                               gCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTaaa                                                  <  2:1670082/150‑1 (MQ=255)                                                                                                   cccACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGgcg                                              >  2:1595038/1‑150 (MQ=255)                                                                                                    ccACGCGCTCGAAGGTACGCTCCTGCAGCGCACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGgcgc                                             >  1:892595/1‑150 (MQ=255)                                                                                                    ccACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGgcgc                                             >  2:1698007/1‑150 (MQ=255)                                                                                                      aCGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGccc                                           >  1:1607482/1‑150 (MQ=255)                                                                                                         cgcTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTtc                                        <  2:1216404/150‑1 (MQ=255)                                                                                                          gcTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTtct                                       >  1:296691/1‑150 (MQ=255)                                                                                                           cTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTtctc                                      <  2:577069/150‑1 (MQ=255)                                                                                                              gAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGTAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGACTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTg                                   >  2:1767425/1‑150 (MQ=255)                                                                                                                      gCTCCTGCAGCGCACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACa                           <  2:892595/150‑1 (MQ=255)                                                                                                                      gCTCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACa                           >  1:278943/1‑150 (MQ=255)                                                                                                                        tCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGa                                                               >  1:1342684/1‑112 (MQ=255)                                                                                                                        tCCTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGa                                                               <  2:1342684/112‑1 (MQ=255)                                                                                                                          cTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTc                       >  2:740736/1‑150 (MQ=255)                                                                                                                          cTGCAGCACACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTc                       >  2:355233/1‑150 (MQ=255)                                                                                                                            gcagcaCACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGc                     >  1:1418570/1‑150 (MQ=255)                                                                                                                               gcaCACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAAc                                                                                                             >  1:588764/1‑59 (MQ=255)                                                                                                                               gcaCACGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAAc                                                                                                             <  2:588764/59‑1 (MQ=255)                                                                                                                                 acacGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTcc                <  2:1607482/150‑1 (MQ=255)                                                                                                                                    cGCAGCAGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTACAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCcagc             <  2:1496960/150‑1 (MQ=255)                                                                                                                                     gcagcaGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCtt                   >  1:1659671/1‑143 (MQ=255)                                                                                                                                     gcagcaGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCtt                   <  2:1659671/143‑1 (MQ=255)                                                                                                                                     gcagcaGCTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCcagca            >  1:83449/1‑150 (MQ=255)                                                                                                                                           gcTTGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCgg      >  2:1467780/1‑150 (MQ=255)                                                                                                                                             ttGACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGcc    <  2:83449/150‑1 (MQ=255)                                                                                                                                               gACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCgg  <  1:740736/150‑1 (MQ=255)                                                                                                                                               gACCTACATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCgg  <  2:1418570/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 ACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGG  >  NC_002947/4964493‑4964784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑