| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 43,461:1 | +A | 17.3% | intergenic (+27/+66) | PP_0040 → / ← cadA‑I | hypothetical protein/cadmium translocating P‑type ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 43,461 | 1 | . | A | 17.3% | 147.6 / 35.1 | 52 | intergenic (+27/+66) | PP_0040/cadA‑I | hypothetical protein/cadmium translocating P‑type ATPase |
| Reads supporting (aligned to +/- strand): ref base . (20/23); new base A (5/4); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
TCGGTTTCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCGCAACAGCG > NC_002947/43316‑43608 | tCGGTTTCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑gcgc < 1:1332931/150‑1 (MQ=255)tCGGTTTCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑gcgc < 1:2800880/150‑1 (MQ=255) tttCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGC‑GATCAGCGCACGa > 1:1526075/1‑150 (MQ=255) ttCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGAc < 1:1261515/150‑1 (MQ=255) cGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGAccc < 1:2016970/150‑1 (MQ=255) aGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGC‑GATCAGCGCACGACCCGta < 2:1526075/150‑1 (MQ=255) ccTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTc > 2:1507779/1‑150 (MQ=255) cTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGC‑GATCAGCGCACGAccc < 2:777224/131‑1 (MQ=255) cTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGC‑GATCAGCGCACGAccc > 1:777224/1‑131 (MQ=255) tCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAg < 2:2680814/145‑1 (MQ=255) tCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAg > 1:2680814/1‑145 (MQ=255) cTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGAtt > 2:979282/1‑150 (MQ=255) aCGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGaa < 1:979282/150‑1 (MQ=255) cGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCCTCGGCATCGATGAGTCACTTGTTGGTGTGGCAGCTCTGCGACTC‑ACTCACGACCCTGACTTCTATGTCAGGTCATTTCCCTTCTTAGCTTCATGATTGAAg < 2:1884392/150‑1 (MQ=255) ggCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAgg > 1:2164341/1‑150 (MQ=255) tCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGta > 2:3027343/1‑98 (MQ=255) tCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGta < 1:3027343/98‑1 (MQ=255) tCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGAt > 2:1498709/1‑150 (MQ=255) tgattGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGt < 2:409674/146‑1 (MQ=255) gCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCg < 1:1067860/78‑1 (MQ=255) gCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCg > 2:1067860/1‑78 (MQ=255) gCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAg < 1:250614/150‑1 (MQ=255) ccTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATc > 2:303990/1‑64 (MQ=255) ccTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATc < 1:303990/64‑1 (MQ=255) ttGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCa > 1:925250/1‑150 (MQ=255) tGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCgg < 1:1823811/142‑1 (MQ=255) tGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCgg > 2:1823811/1‑142 (MQ=255) aaGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAg < 1:1498709/150‑1 (MQ=255) cTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAgg > 1:2269999/1‑139 (MQ=255) cTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAgg < 2:2269999/139‑1 (MQ=255) cTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGgcgc > 1:2673700/1‑150 (MQ=255) gaTCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGtt < 2:1409370/149‑1 (MQ=255) aTCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTg < 2:925250/150‑1 (MQ=255) tCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGgc < 1:1009671/138‑1 (MQ=255) tCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGgc > 2:1009671/1‑138 (MQ=255) gggATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAg < 2:2673700/150‑1 (MQ=255) ggTGAGAGAGAAGTACGTGTCGTGGGCTGATC‑GCTCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACa < 2:2023464/149‑1 (MQ=255) tGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAgt > 1:471110/1‑150 (MQ=255) gggTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGcc > 2:44552/1‑144 (MQ=255) gggTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGcc < 1:44552/144‑1 (MQ=255) ggTCGTGCGC‑GATCAGCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATgg > 2:2444208/1‑150 (MQ=255) gTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCt < 2:488401/59‑1 (MQ=255) gTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCt > 1:488401/1‑59 (MQ=255) tCGTGCGCTGATC‑GCGCACGACCCGTAGTTCGACTTCAGGTCATTTCCCTGCTTCGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGAt > 1:69776/1‑150 (MQ=255) tCGTGCGC‑GATCAGCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAg < 2:2667568/82‑1 (MQ=37) tCGTGCGC‑GATCAGCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAg > 1:2667568/1‑82 (MQ=37) tCGTGCGC‑GATCAGCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAggcga > 2:2477649/1‑125 (MQ=255) tCGTGCGC‑GATCAGCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAggcga < 1:2477649/125‑1 (MQ=255) cGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATc < 2:2164341/150‑1 (MQ=255) gTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCAt < 2:1152600/144‑1 (MQ=255) gTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCAt > 1:1152600/1‑144 (MQ=255) tGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAg > 2:2962255/1‑72 (MQ=255) tGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAg < 1:2962255/72‑1 (MQ=255) gcTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATTGATCGCaa < 2:2803085/149‑1 (MQ=255) gcTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATTGATCGCaa > 1:2803085/1‑149 (MQ=255) aTC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCGCAACAGCg > 1:1482518/1‑150 (MQ=255) | TCGGTTTCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGATC‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCGCAACAGCG > NC_002947/43316‑43608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |