Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,727,163:1 +T 6.1% coding (333/645 nt) PP_3292 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,727,1631.T 6.1% 394.7 / 19.2 98coding (333/645 nt)PP_3292membrane protein
Reads supporting (aligned to +/- strand):  ref base . (41/51);  new base T (3/3);  total (44/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

TAGGCTGGGCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCC  >  NC_002947/3727018‑3727311
                                                                                                                                                  |                                                                                                                                                    
tAGGCTGGGCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑Gccc                                                                                                                                                  >  1:2276848/1‑150 (MQ=255)
tAGGCTGGGCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑Gccc                                                                                                                                                  >  1:262120/1‑150 (MQ=255)
  ggCTGGGCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCctg                                                                                                                                                >  1:1891775/1‑150 (MQ=255)
   gCTGGGCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCctgc                                                                                                                                               <  2:2770134/150‑1 (MQ=255)
      gggCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTgg                                                                                                                                            <  2:902468/150‑1 (MQ=255)
      gggCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTgg                                                                                                                                            <  2:2435558/150‑1 (MQ=255)
      gggCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTgg                                                                                                                                            <  1:1867453/150‑1 (MQ=255)
                   gggCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTgg                                                                                                                                            <  2:989292/137‑1 (MQ=255)
                   gggCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTgg                                                                                                                                            >  1:989292/1‑137 (MQ=255)
                     gCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCtttt                                                                                                                             <  2:1891775/150‑1 (MQ=255)
                     gCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCtttt                                                                                                                             <  1:1971429/150‑1 (MQ=255)
                     gCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCtttt                                                                                                                             <  2:2276848/150‑1 (MQ=255)
                     gCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCtttt                                                                                                                             <  2:462075/150‑1 (MQ=255)
                     gCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCtttt                                                                                                                             <  1:2624674/150‑1 (MQ=255)
                     gCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACAGCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAcaacag                                                                                                                             <  2:1464908/150‑6 (MQ=255)
                       gTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtgg                                                                                                                           <  1:818418/150‑1 (MQ=255)
                        tGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtggc                                                                                                                          >  2:313454/1‑150 (MQ=255)
                        tGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtggc                                                                                                                          <  1:2156002/150‑1 (MQ=255)
                        tGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGACAA‑GCCTTGCTGGGCACAGGCACCTTTtggc                                                                                                                          <  1:2860092/150‑1 (MQ=255)
                         gAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGACTCGAACATCCTGCTGGTGCTGCTGTTGTGCCTG‑CCCCTGCTGGGCCCCGGCCCCCTCtggct                                                                                                                         >  2:1319310/1‑150 (MQ=255)
                           aGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtggctgg                                                                                                                       <  2:1478547/150‑1 (MQ=255)
                           aGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtggctgg                                                                                                                       <  1:313454/150‑1 (MQ=255)
                            gcgcACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtggctggc                                                                                                                      >  2:881469/1‑150 (MQ=255)
                               cACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAgg                                                                                                                   <  1:2274531/150‑1 (MQ=255)
                                aCCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAggg                                                                                                                  >  2:2579672/1‑150 (MQ=255)
                                 ccGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCctgct                                                                                                                                              <  2:22554/121‑1 (MQ=255)
                                 ccGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCctgct                                                                                                                                              >  1:22554/1‑121 (MQ=255)
                                  cGTTGGCAAAGCCATTTTCAACGGGCAGGAGAAAGGCTAGCAGGCTAGACTCGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTGGGCTGGCAGGgcg                                                                                                                <  2:671760/150‑1 (MQ=255)
                                       gCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAg                                                                                                           >  1:121224/1‑150 (MQ=255)
                                        cAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGt                                                                                                          >  1:2133195/1‑150 (MQ=255)
                                        cAAAGCCATTTTCAACGGCCAGGAAACAGGCTGGCAGGCCAGACTGTCGGTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGt                                                                                                          >  2:895479/1‑150 (MQ=255)
                                          aaGCCGTTTTCAACGGCGAGGAGGCAGGCTGGCAGGCCAGACTGGCGTTCCGTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGGGCTGGTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGa                                                                                                        <  1:1319310/150‑1 (MQ=255)
                                             ccATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCt                                                                                                                                >  2:1423319/1‑123 (MQ=255)
                                             ccATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCt                                                                                                                                <  1:1423319/123‑1 (MQ=255)
                                              cATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCa                                                                                                    >  2:2420731/1‑150 (MQ=255)
                                                ttttCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGa                                                                                                        <  2:2268392/144‑1 (MQ=255)
                                                ttttCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGa                                                                                                        >  1:2268392/1‑144 (MQ=255)
                                                     aaCGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGgc                                                                                                                 <  2:1845145/130‑1 (MQ=255)
                                                     aaCGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGgc                                                                                                                 >  1:1845145/1‑130 (MQ=255)
                                                     aaCGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTg                                                                                             <  1:2763921/150‑1 (MQ=255)
                                                      aCGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGCCGCAGATCGTgg                                                                                            <  1:1189516/150‑1 (MQ=255)
                                                      aCGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTgg                                                                                            <  1:2579672/150‑1 (MQ=255)
                                                           cAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAcaac                                                                                                                               >  2:1821802/1‑107 (MQ=38)
                                                           cAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAcaac                                                                                                                               <  1:1821802/110‑4 (MQ=38)
                                                            aGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCGGATCGTgg                                                                                            >  2:933323/1‑144 (MQ=255)
                                                            aGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCGGATCGTgg                                                                                            <  1:933323/144‑1 (MQ=255)
                                                                gaCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGa                                                                                  >  2:2810812/1‑150 (MQ=255)
                                                                 aCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAg                                                                                 <  2:2025193/150‑1 (MQ=255)
                                                                 aCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAg                                                                                 <  2:1136846/150‑1 (MQ=255)
                                                                 aCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAg                                                                                 >  2:1626828/1‑150 (MQ=255)
                                                                  cAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGTCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAgg                                                                                <  2:2721318/150‑1 (MQ=255)
                                                                   aGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAcaa                                                                                                                                <  1:714091/101‑3 (MQ=38)
                                                                   aGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAcaa                                                                                                                                >  2:714091/1‑99 (MQ=37)
                                                                    ggCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGcc                                                                              <  1:3054666/150‑1 (MQ=255)
                                                                     gCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCa                                                                             >  2:888713/1‑150 (MQ=255)
                                                                      cTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCaa                                                                            >  2:2976411/1‑150 (MQ=255)
                                                                           aGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGc                                                                       <  1:357362/150‑1 (MQ=255)
                                                                                    tGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTg‑g                                                                                                                                                     >  1:274994/1‑63 (MQ=255)
                                                                                    tGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTg‑g                                                                                                                                                     <  2:274994/63‑1 (MQ=255)
                                                                                        gTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGa                                                          >  2:1559017/1‑150 (MQ=255)
                                                                                        gTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGa                                                          <  1:1308200/150‑1 (MQ=255)
                                                                                        gTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGa                                                          <  1:3047631/150‑1 (MQ=255)
                                                                                            cTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGTAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGAtata                                                      >  1:2797079/1‑150 (MQ=255)
                                                                                             ttCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCtttt                                                                                                                             >  1:2429667/1‑78 (MQ=255)
                                                                                             ttCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCtttt                                                                                                                             <  2:2429667/78‑1 (MQ=255)
                                                                                              tCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATAcc                                                    <  1:881469/150‑1 (MQ=255)
                                                                                                gCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGATTTCCGAGGTCAACCTGCAGGCTCAAGTGGATATACCCg                                                  >  2:2254343/1‑150 (MQ=255)
                                                                                                  tCAGTGGCTCGGGACTCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTCCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAGCTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGaa                                                <  1:2930677/150‑1 (MQ=255)
                                                                                                  tCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGa                                                                                  >  2:994959/1‑116 (MQ=255)
                                                                                                  tCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGaa                                                <  2:2794087/150‑1 (MQ=255)
                                                                                                  tCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCATG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGa                                                                                  <  1:994959/116‑1 (MQ=255)
                                                                                                   cAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTCGCTG‑GCCCTGCTGGGCAGAGGCACCTTTTGGCTGGCAGCGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAg                                               <  2:262120/150‑1 (MQ=255)
                                                                                                                 tCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAg                                                                     >  1:599932/1‑114 (MQ=255)
                                                                                                                 tCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAg                                                                     <  2:599932/114‑1 (MQ=255)
                                                                                                                 tCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCAcc                                 >  1:2632625/1‑150 (MQ=255)
                                                                                                                 tCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCAcc                                 >  2:2539616/1‑150 (MQ=255)
                                                                                                                   gAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGAATGCTCAAGTGGATATACCCGAAGATGAAAAAcagatcgg                               >  1:1570229/1‑143 (MQ=255)
                                                                                                                    aaCATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACcgcg                              >  2:2124940/1‑150 (MQ=255)
                                                                                                                    aaCATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACcgcg                              >  1:2911626/1‑150 (MQ=255)
                                                                                                                     aCATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCt                                                                  <  1:1183556/113‑1 (MQ=255)
                                                                                                                     aCATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCt                                                                  >  2:1183556/1‑113 (MQ=255)
                                                                                                                     aCATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCgcgc                             <  2:2911626/150‑1 (MQ=255)
                                                                                                                       aTCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCtt                           >  2:180971/1‑150 (MQ=255)
                                                                                                                           tgctgGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCg                       <  1:895479/150‑1 (MQ=255)
                                                                                                                                    tgCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAg                                                                                 >  1:625515/1‑83 (MQ=255)
                                                                                                                                    tgCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAg                                                                                 <  2:625515/83‑1 (MQ=255)
                                                                                                                                    tgCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCgg              >  2:2724557/1‑150 (MQ=255)
                                                                                                                                        gttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGgt             <  1:893579/147‑1 (MQ=255)
                                                                                                                                        gttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGgt             >  2:893579/1‑147 (MQ=255)
                                                                                                                                        gttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGtgtg          <  2:121224/150‑1 (MQ=255)
                                                                                                                                         ttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGc         <  1:2420731/150‑1 (MQ=255)
                                                                                                                                           gtgCCTGTGCCCAGCAGGGC‑CAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCAc       >  2:1599673/1‑150 (MQ=255)
                                                                                                                                           gtgCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCAc       >  1:942300/1‑150 (MQ=255)
                                                                                                                                             gCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGt     <  1:2124940/150‑1 (MQ=255)
                                                                                                                                             gCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGt     <  2:2133195/150‑1 (MQ=255)
                                                                                                                                              ccTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACATGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTg    >  1:832697/1‑150 (MQ=255)
                                                                                                                                                tG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGTAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGcc  <  2:2797079/150‑1 (MQ=255)
                                                                                                                                                tG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGaaaaa                                       >  2:2471022/1‑113 (MQ=255)
                                                                                                                                                tG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGaaaaa                                       <  1:2471022/113‑1 (MQ=255)
                                                                                                                                                tG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGcc  >  1:569706/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TAGGCTGGGCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCC  >  NC_002947/3727018‑3727311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑