| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,184,594 | G→C | 22.2% | intergenic (‑204/‑208) | mltF ← / → purL | membrane‑bound lytic murein transglycosylase F/phosphoribosylformylglycinamidine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,184,594 | 0 | G | C | 22.2% | 102.7 / 23.6 | 45 | intergenic (‑204/‑208) | mltF/purL | membrane‑bound lytic murein transglycosylase F/phosphoribosylformylglycinamidine synthase |
| Reads supporting (aligned to +/- strand): ref base G (20/15); new base C (7/3); total (27/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCG > NC_002947/1184452‑1184735 | tAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGgcgc > 2:3072041/1‑150 (MQ=255) aGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCt < 2:328530/150‑1 (MQ=255) gtggggTGTTTGGTGGGGTGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCCATGAGATCGAGCGCCGCGCGGGCGGCGCTc < 2:2635377/148‑1 (MQ=38) gCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTc < 1:3072041/150‑1 (MQ=255) ttGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGgcg < 2:1176985/150‑1 (MQ=255) gggAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGa > 1:2236367/1‑150 (MQ=255) gggAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCG‑CAGGTGTCCGGCTCGACACATTCTGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCATAGGCGCTGaa < 1:2179182/150‑1 (MQ=11) ggggggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCg < 2:1633412/130‑1 (MQ=255) ggggggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCg > 1:1633412/1‑130 (MQ=255) gggggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAAtgtg > 2:353185/1‑150 (MQ=255) aTTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAg < 1:1187305/150‑1 (MQ=255) gggCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCgcggg < 1:2009309/106‑1 (MQ=255) gggCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCgcggg > 2:2009309/1‑106 (MQ=255) gTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTc < 2:2236367/150‑1 (MQ=255) cgcCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGggcggc > 1:571350/1‑78 (MQ=255) cgcCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGggcggc < 2:571350/78‑1 (MQ=255) ccAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGAt > 1:2676919/1‑110 (MQ=255) ccAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGAt < 2:2676919/110‑1 (MQ=255) gtgtCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCg > 2:1859267/1‑150 (MQ=255) tCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGt < 2:455605/108‑1 (MQ=255) tCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGt > 1:455605/1‑108 (MQ=255) tCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACctct < 2:531427/126‑1 (MQ=255) tCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACctct > 1:531427/1‑126 (MQ=255) cGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTcc > 2:348953/1‑150 (MQ=255) cTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTG‑GGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGt > 1:6957/1‑143 (MQ=255) cTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTG‑GGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGt < 2:6957/143‑1 (MQ=255) ttAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGa > 2:906252/1‑80 (MQ=255) ttAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGa < 1:906252/80‑1 (MQ=255) ttCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAg > 2:1069416/1‑150 (MQ=255) gcCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg < 1:353185/150‑1 (MQ=255) tgtGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCATAGGCGCTGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCCGACACCTCTGAAAACTGACCCCGAAGTCCGGATCCGCCCCCCGGCACGCGTGGCtt > 2:562926/3‑150 (MQ=25) tGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCg < 2:625150/92‑1 (MQ=255) tGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCg > 1:625150/1‑92 (MQ=255) gaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGa < 1:1136294/52‑1 (MQ=255) gaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGa > 2:1136294/1‑52 (MQ=255) atggAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCa > 1:3063095/4‑150 (MQ=255) aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCa > 1:987487/1‑150 (MQ=255) tCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACGGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAg > 1:186273/1‑150 (MQ=255) tCGAGCGCCGCCCGCGCGGCGCTCGCTGGCACATGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAg > 1:2384219/1‑150 (MQ=255) cGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAgg > 1:2451077/1‑150 (MQ=255) gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGt > 2:330834/1‑150 (MQ=255) gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGAt < 1:2113064/71‑1 (MQ=255) gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGAt > 2:2113064/1‑71 (MQ=21) gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGt > 2:2725377/1‑150 (MQ=255) cgccgcGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGcc > 1:1359002/1‑150 (MQ=255) gccgcGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCg > 1:1783631/1‑150 (MQ=255) gccgcGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCg > 2:864888/1‑150 (MQ=255) ccgcGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcc > 1:2307004/1‑100 (MQ=255) ccgcGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcc < 2:2307004/100‑1 (MQ=255) | TAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCG > NC_002947/1184452‑1184735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |