Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,344,121 A→C 5.5% C8G (TGC→GGC)  PP_1168 ← TRAP dicarboxylate transporter subunit DctQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,344,1210AC 5.5% 373.3 / 10.6 128C8G (TGC→GGC) PP_1168TRAP dicarboxylate transporter subunit DctQ
Reads supporting (aligned to +/- strand):  ref base A (68/53);  new base C (3/4);  total (71/57)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

Only 100 of 128 total aligned reads displayed.

CGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAATGAACTCGACA  >  NC_002947/1343973‑1344266
                                                                                                                                                    |                                                                                                                                                 
cGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATTACCAAAGCGGCAGGGCGGCGGGCGCCGCAc                                                                                                                                                  >  1:2417744/1‑150 (MQ=255)
 gggCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGTGCGGCGCCCGCCGCCCt                                                                                                                                                 <  1:2759157/150‑1 (MQ=255)
    cGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGcc                                                                                                                                              <  2:1554698/150‑1 (MQ=255)
    cGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGcc                                                                                                                                              <  1:1376961/150‑1 (MQ=255)
           gCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCg                                                                                                                                       >  1:1350649/1‑150 (MQ=255)
             cccACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAt                                                                                                                                     >  2:2476248/1‑150 (MQ=255)
              ccACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAtt                                                                                                                                    >  2:2926432/1‑150 (MQ=255)
              ccACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAtt                                                                                                                                    >  1:103533/1‑150 (MQ=255)
               cACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTg                                                                                                                                   >  1:2100411/1‑150 (MQ=255)
                aCGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTgg                                                                                                                                  >  2:2703979/1‑150 (MQ=255)
                 cGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTggg                                                                                                                                 <  1:2445355/150‑1 (MQ=255)
                    gatgatCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCaa                                                                                                                              >  2:1783366/1‑150 (MQ=255)
                      tgatCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAgg                                                                                                                            <  2:103533/150‑1 (MQ=255)
                        atCAGCGTCACGCCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGTGTGTCGTTCACGATCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGTCAGGGCCGCGGGCGCCGCACTGCCGAGTTCGATTGGGCAAGggc                                                                                                                          <  2:452440/150‑1 (MQ=255)
                         tCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGggcg                                                                                                                         >  1:1248742/1‑150 (MQ=255)
                         tCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGggca                                                                                                                         <  2:2100411/150‑2 (MQ=255)
                             cGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTg                                                                                                                     <  2:1876814/150‑1 (MQ=255)
                                     ccAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCATGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGa                                                                                                             >  1:578356/1‑150 (MQ=255)
                                     ccAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGa                                                                                                             >  2:2782309/1‑150 (MQ=255)
                                      gaGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGAc                                                                                                            >  2:1722395/2‑150 (MQ=255)
                                       aGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGt                                                                                                                    >  1:385378/1‑141 (MQ=255)
                                       aGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGt                                                                                                                    <  2:385378/141‑1 (MQ=255)
                                            cGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTc                                                                                                      <  1:285874/150‑1 (MQ=255)
                                                   cccGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCTGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGc                                                                                               <  1:576694/150‑1 (MQ=255)
                                                   cccGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCTGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGc                                                                                               >  2:576694/1‑150 (MQ=255)
                                                   cccGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGATAGGGCAGCGTGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGc                                                                                               >  1:369873/1‑150 (MQ=255)
                                                     cgcgATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAggg                                                                                                                           <  1:2584704/120‑1 (MQ=255)
                                                     cgcgATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCtt                                                                                             >  1:851032/1‑150 (MQ=255)
                                                     cgcgATCCAGAAAAAGCTGGCGTACTGCGGGTCGGTCACGCGCATGGAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTACCTAGGTCGATTGGTCAAGGGCGGCTGTTACTGGACGGCGTCGagatcgga                                                                                             >  2:2969649/1‑143 (MQ=255)
                                                        gATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATTACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcat                                                                                          <  2:2417744/150‑1 (MQ=255)
                                                         aTCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAggg                                                                                                                           <  1:2139282/116‑1 (MQ=255)
                                                         aTCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAggg                                                                                                                           >  2:2139282/1‑116 (MQ=255)
                                                         aTCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTAAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcatc                                                                                         <  1:487810/150‑1 (MQ=255)
                                                               aTGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcatc                                                                                         <  2:2220848/144‑1 (MQ=255)
                                                                 gCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCATGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGc                                                                                 <  2:578356/150‑1 (MQ=255)
                                                                   aaCTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGt                                                                               >  2:1018365/1‑150 (MQ=255)
                                                                   aaCTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGt                                                                               >  2:883207/1‑150 (MQ=255)
                                                                               gCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCg                                                                                <  1:1088084/137‑1 (MQ=255)
                                                                               gCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCg                                                                                <  1:1087930/137‑1 (MQ=255)
                                                                               gCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCg                                                                                >  2:1087930/1‑137 (MQ=255)
                                                                                  tgtCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGc                                                                                                                                               <  1:491422/71‑1 (MQ=255)
                                                                                  tgtCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGc                                                                                                                                               >  2:491422/1‑71 (MQ=255)
                                                                                   gtCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTAttt                                                               >  1:605233/1‑150 (MQ=255)
                                                                                     cGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTAttttt                                                             <  2:1561249/150‑1 (MQ=255)
                                                                                      gTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTc                                                            >  2:705868/1‑150 (MQ=255)
                                                                                       ttCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTcg                                                           >  2:843895/1‑150 (MQ=255)
                                                                                       ttCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGCCAGGGCGGCGGGCGCCGCACTACCGAGGCCGATTGGGCAAGGGCGGCCGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTcg                                                           >  2:2874717/1‑150 (MQ=255)
                                                                                        tCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTgcgc                                                                        <  1:1688445/136‑1 (MQ=255)
                                                                                        tCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTcgc                                                          <  2:1248742/150‑1 (MQ=255)
                                                                                        tCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAGTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTgcgc                                                                        >  2:1688445/1‑136 (MQ=255)
                                                                                          aCGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGt                                                        >  2:1206245/1‑150 (MQ=255)
                                                                                              tCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcat                                                                                          <  1:2126187/112‑1 (MQ=255)
                                                                                              tCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcat                                                                                          >  2:2126187/1‑112 (MQ=255)
                                                                                              tCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCg                                                    >  1:2261526/1‑150 (MQ=255)
                                                                                              tCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCg                                                    >  2:3019170/1‑150 (MQ=255)
                                                                                              tCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCg                                                    >  1:2865034/1‑150 (MQ=255)
                                                                                              tCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCg                                                    >  2:397266/1‑150 (MQ=255)
                                                                                              cacagAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCCTCAGGTCGGCGTACTGAGAGCAGTGTTTTTGGCGTACCg                                                    >  1:885660/5‑150 (MQ=255)
                                                                                                aGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGaa                                                  <  1:1407523/150‑1 (MQ=255)
                                                                                                 ggAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCt                                                                                                                      <  1:897224/81‑1 (MQ=255)
                                                                                                 ggAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCt                                                                                                                      >  2:897224/1‑81 (MQ=255)
                                                                                                  gAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGt                                                                                                                                         <  1:2168924/61‑1 (MQ=255)
                                                                                                  gAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGt                                                                                                                                         >  2:2168924/1‑61 (MQ=255)
                                                                                                     aaGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGt                                             <  2:2865034/150‑1 (MQ=255)
                                                                                                          aTTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTAGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGttctac                                        >  1:538990/1‑145 (MQ=255)
                                                                                                           tttCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcatc                                                                                         <  1:2788856/100‑1 (MQ=255)
                                                                                                           tttCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcatc                                                                                         >  2:2788856/1‑100 (MQ=255)
                                                                                                           tttCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtc                                       <  2:605233/150‑1 (MQ=255)
                                                                                                            ttCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtcg                                      <  2:1255893/150‑1 (MQ=255)
                                                                                                             tCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTAt                                                                 >  1:1853735/1‑122 (MQ=255)
                                                                                                               aTAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAg                                   >  1:2398652/1‑150 (MQ=255)
                                                                                                                   gCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtcg                                      >  2:1388944/1‑143 (MQ=255)
                                                                                                                   gCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtcg                                      <  1:1388944/143‑1 (MQ=255)
                                                                                                                   gCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACg                               >  2:1682815/1‑150 (MQ=255)
                                                                                                                     aTCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGc                             >  2:84306/1‑150 (MQ=255)
                                                                                                                     aTCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGc                             >  1:2876001/1‑150 (MQ=255)
                                                                                                                      tCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGct                            <  1:84306/150‑1 (MQ=255)
                                                                                                                          cAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGa                                                                                                    >  2:2358929/1‑74 (MQ=255)
                                                                                                                          cAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGa                                                                                                    <  1:2358929/74‑1 (MQ=255)
                                                                                                                          cAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGTcttctt                        <  2:1443243/150‑1 (MQ=255)
                                                                                                                          cAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGcttctt                        <  1:843895/150‑1 (MQ=255)
                                                                                                                           aaaGCGGCAGGGCGGCGGGCGCCGAACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATa                                                                                                  <  2:92692/75‑1 (MQ=255)
                                                                                                                           aaaGCGGCAGGGCGGCGGGCGCCGAACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATa                                                                                                  >  1:92692/1‑75 (MQ=255)
                                                                                                                            aaGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGt                      <  2:2876001/150‑1 (MQ=255)
                                                                                                                            aaGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGt                      <  1:883207/150‑1 (MQ=255)
                                                                                                                             ctcGGCAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGAAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTc                     >  1:1953202/3‑150 (MQ=255)
                                                                                                                               cGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGa                   <  1:3019170/150‑1 (MQ=255)
                                                                                                                                 gCAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATAAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACg                 >  1:2062856/1‑150 (MQ=255)
                                                                                                                                 gCAGTGCGGCGCCCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACg                 >  2:2736151/1‑150 (MQ=255)
                                                                                                                                  cAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACgg                <  2:2062856/150‑1 (MQ=255)
                                                                                                                                   aGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGt               >  1:2290061/1‑150 (MQ=255)
                                                                                                                                    gggCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTa              <  1:1682815/150‑1 (MQ=255)
                                                                                                                                      gcggcgGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAAt            >  1:1632993/1‑150 (MQ=255)
                                                                                                                                         gcgggcgCCTCATTGCCGAGGTCGATTGGGCAAGCGCGGCTGTTACTGGACGTCGTCGATACGCTTCATCATGTCGGCTTACTGCGCTCCGTATTTTTCGCGTACCGAAGCCGTCGCGTCGTAGAACGGCTTCTTGTCGACGGTAATGaa         <  1:314527/150‑1 (MQ=255)
                                                                                                                                           gggcgCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAATGAACt       >  2:2979352/1‑150 (MQ=255)
                                                                                                                                            ggcgCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAATGAACTc      <  1:2782309/150‑1 (MQ=255)
                                                                                                                                            ggcgCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAATGAACTc      <  1:2979352/150‑1 (MQ=255)
                                                                                                                                            ggcgCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTA‑TTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAATGAACTCg     >  1:2277890/1‑150 (MQ=255)
                                                                                                                                              cgccgcACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCATATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAATGAACTc      <  2:708362/148‑1 (MQ=255)
                                                                                                                                                ccgcACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTCAGGGGCGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAATGAACTCGaca  <  1:809261/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGGGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAATGAACTCGACA  >  NC_002947/1343973‑1344266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑