Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,707,288 A→G 7.5% intergenic (+64/+78) PP_1501 → / ← PP_1502 hypothetical protein/OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,707,2880AG 7.5% 231.7 / 7.1 80intergenic (+64/+78)PP_1501/PP_1502hypothetical protein/OmpA family protein
Reads supporting (aligned to +/- strand):  ref base A (34/40);  new base G (3/3);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

AAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCG  >  NC_002947/1707141‑1707437
                                                                                                                                                   |                                                                                                                                                      
aaaGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcaca                                                                                                                                                      >  1:1498933/1‑150 (MQ=255)
          atctcGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAGACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGNGAGATCNAGCGCCGCGCGGNCGGCGCTCGATCTCACAGGCGGCacaa                                                                                                                                            <  1:2715366/146‑1 (MQ=255)
           cGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCacaac                                                                                                                                           >  1:2668655/1‑150 (MQ=255)
               gTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAGACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCacaacagat                                                                                                                                       >  2:2715366/1‑147 (MQ=255)
                   tggtggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTa                                                                                                                                       >  1:2785201/1‑146 (MQ=255)
                   tggtggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTa                                                                                                                                       <  2:2785201/146‑1 (MQ=255)
                   tggtggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCgg                                                                                                                                   <  1:894143/150‑1 (MQ=255)
                      tggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACAtt                                                                                                                                        <  2:1169582/142‑1 (MQ=255)
                      tggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACAtt                                                                                                                                        >  1:1169582/1‑142 (MQ=255)
                        gACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAAc                                                                                                                              >  1:498698/1‑150 (MQ=255)
                          cAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACaa                                                                                                                            >  2:2572145/1‑150 (MQ=255)
                           aaCCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACaaa                                                                                                                           >  1:4566/1‑150 (MQ=255)
                           aaCCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACaaa                                                                                                                           <  2:1498933/150‑1 (MQ=255)
                              cGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGaa                                                                                                                        >  2:239135/1‑150 (MQ=255)
                               gCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGaaa                                                                                                                       >  2:1861042/1‑150 (MQ=255)
                               gCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGaaa                                                                                                                       >  2:1637101/1‑150 (MQ=255)
                                cAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGaaaa                                                                                                                      <  2:1461951/150‑1 (MQ=255)
                                         ggTGGGCTGCAGGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCCAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑t                                                                                                            <  1:1347902/150‑1 (MQ=255)
                                                   aTGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCggg                                                                                                  <  1:2572145/150‑1 (MQ=255)
                                                   aTGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCggg                                                                                                  <  2:2668655/150‑1 (MQ=255)
                                                         gAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGt                                                                                            >  2:1793885/1‑150 (MQ=255)
                                                          gatctacacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGAACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTc                                                                                           <  1:1615786/145‑1 (MQ=255)
                                                           ggAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTcc                                                                                          >  1:976880/1‑150 (MQ=255)
                                                              aaCACCGCGGCAGGTTCAGTGAGGTTTCGGCGCAGCATTTTTCGCGCCCGCGAGATCGGGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGA‑CGCGGGGCttttctttg                                                                                       <  1:1430625/150‑8 (MQ=38)
                                                              aaCACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCttt                                                                                       >  2:1001660/1‑150 (MQ=255)
                                                               tcacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTc                                                                                      <  1:2190361/149‑1 (MQ=255)
                                                               acacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTc                                                                                      <  2:564498/150‑1 (MQ=255)
                                                               acacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTc                                                                                      <  2:4566/150‑1 (MQ=255)
                                                               acacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTc                                                                                      <  2:2643438/150‑1 (MQ=255)
                                                                cacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCa                                                                                     <  1:1861042/150‑1 (MQ=255)
                                                                 acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTc                                                                                           >  1:2612711/1‑143 (MQ=255)
                                                                 acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTc                                                                                           <  2:2612711/143‑1 (MQ=255)
                                                                 acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAg                                                                                    <  1:1637101/150‑1 (MQ=255)
                                                                 acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAg                                                                                    >  1:2431818/1‑150 (MQ=255)
                                                                   cgcgGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcaca                                                                                                                                                      <  2:1431579/83‑1 (MQ=255)
                                                                   cgcgGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcaca                                                                                                                                                      >  1:1431579/1‑83 (MQ=255)
                                                                   cgcgGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCacaaca                                                                                                                                          <  1:367979/95‑1 (MQ=255)
                                                                   cgcgGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCacaaca                                                                                                                                          >  2:367979/1‑95 (MQ=255)
                                                                   cgcgGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCt                                                                                  >  1:2334980/1‑150 (MQ=255)
                                                                    gcgGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTa                                                                                 <  2:1053576/150‑1 (MQ=255)
                                                                    gcgGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTa                                                                                 <  2:784519/150‑1 (MQ=255)
                                                                     cgGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTag                                                                                >  2:1002327/1‑150 (MQ=255)
                                                                                gTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATcgcg                                                                                                    <  1:48806/119‑1 (MQ=255)
                                                                                gTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATcgcg                                                                                                    >  2:48806/1‑119 (MQ=255)
                                                                                gTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAgc                                                                     <  2:2681856/150‑1 (MQ=255)
                                                                                 tGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTc                                                                                           >  1:672164/1‑127 (MQ=255)
                                                                                 tGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTc                                                                                           <  2:672164/127‑1 (MQ=255)
                                                                                     gTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGc                                                                                                <  2:2287376/118‑1 (MQ=255)
                                                                                     gTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGc                                                                                                >  1:2287376/1‑118 (MQ=255)
                                                                                         cGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCt                                                            <  1:1002327/150‑1 (MQ=255)
                                                                                         cGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCt                                                            <  2:309276/150‑1 (MQ=255)
                                                                                                gcaTTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCAc                                                     <  1:1001660/150‑1 (MQ=255)
                                                                                                       tGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAAATTTGCGCACCGAGCgt                                              <  2:2431818/150‑1 (MQ=255)
                                                                                                        ggCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGaaaa                                                                                                                      <  1:2682844/78‑1 (MQ=255)
                                                                                                        ggCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGaaaa                                                                                                                      >  2:2682844/1‑78 (MQ=255)
                                                                                                         gcgcCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATcgcg                                                                                                    >  1:256374/1‑94 (MQ=255)
                                                                                                         gcgcCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATcgcg                                                                                                    <  2:256374/94‑1 (MQ=255)
                                                                                                           gcCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtggtg                                          <  1:1797721/150‑1 (MQ=255)
                                                                                                                   gaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGACCAAAGAAAAGCCCCACG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGc                                  >  1:1909762/1‑150 (MQ=255)
                                                                                                                   gaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCGCAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGc                                  >  2:1007543/1‑150 (MQ=255)
                                                                                                                    aTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCt                                 >  1:554095/1‑150 (MQ=255)
                                                                                                                    aTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCt                                 <  2:498698/150‑1 (MQ=255)
                                                                                                                     tCGAGCGCCGCCCGCGCGGCGCTCGATCTCGCAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTg                                >  2:2586211/1‑150 (MQ=255)
                                                                                                                     tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑Tc                                                                                                           <  2:1832817/75‑1 (MQ=25)
                                                                                                                     tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑Tc                                                                                                           >  1:1832817/1‑75 (MQ=25)
                                                                                                                      cGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCt                                                            >  2:1471513/1‑121 (MQ=255)
                                                                                                                      cGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCt                                                            <  1:1471513/121‑1 (MQ=255)
                                                                                                                      cGAGCGCCGCGCGGGCGGAGATCGATCTCATGGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTgg                               <  2:2334980/150‑1 (MQ=255)
                                                                                                                       gatcgccgcCCGCGCGGCGCTCGATCTCGCGGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtggt                                           >  2:2052204/4‑137 (MQ=255)
                                                                                                                       gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGt                              >  2:2352756/1‑150 (MQ=255)
                                                                                                                       gAGCGCCGCCCGCGCGGCGCTCGATCTCGCGGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtggt                                           <  1:2052204/137‑1 (MQ=39)
                                                                                                                         gcgcCGCGCGGGCGGAGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCAACGAGCGTGGTGCCACCGGCTGGTTg                            >  1:1489860/1‑150 (MQ=255)
                                                                                                                               cccgcgcggcgCTCGATCTCGCAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTg                      <  1:1007543/145‑1 (MQ=255)
                                                                                                                                      ggcgCTCGATCTCGCAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATgg               <  1:2586211/150‑1 (MQ=255)
                                                                                                                                      ggcgCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATgg               <  1:1793885/150‑1 (MQ=255)
                                                                                                                                      ggagcTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCAACGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATgg               <  2:1489860/147‑1 (MQ=255)
                                                                                                                                           tCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTTTCtttg                                                                                       >  1:1875483/1‑72 (MQ=255)
                                                                                                                                             gATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgt                                              <  2:1261233/112‑1 (MQ=255)
                                                                                                                                             gATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgt                                              >  1:1261233/1‑112 (MQ=255)
                                                                                                                                             gATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTc        >  1:2031672/1‑150 (MQ=255)
                                                                                                                                             gATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTc        >  1:2031799/1‑150 (MQ=255)
                                                                                                                                                 tcACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTg                                                           <  2:2490437/95‑1 (MQ=255)
                                                                                                                                                 tcACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTg                                                           >  1:2490437/1‑95 (MQ=255)
                                                                                                                                                  cacaGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCAccc   <  1:2036981/150‑1 (MQ=255)
                                                                                                                                                   acaGGCGGCACAACATTATCGGCGAACAACAAACTGCCCCTCG‑TGGAGCGCTGGGCTTGTTGTTTCAGCTAGGAACTGCTGCAGGCGCTCGGTACACACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGATACCTCCACCCg  >  1:2995604/1‑150 (MQ=255)
                                                                                                                                                   acaGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCGTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCg  >  2:1993478/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
AAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCG  >  NC_002947/1707141‑1707437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑