Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,001,858 C→G 6.8% A180G (GCC→GGC)  PP_2625 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,001,8580CG 6.8% 423.1 / 18.0 132A180G (GCC→GGC) PP_2625hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (55/68);  new base G (5/4);  total (60/72)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 135 total aligned reads displayed.

AATCAGTTGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCAC  >  NC_002947/3001709‑3002002
                                                                                                                                                     |                                                                                                                                                
aaTCAGTTGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGc                                                                                                                                                  >  2:1751174/1‑150 (MQ=255)
      ttGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcg                                                                                                                                            >  2:986195/1‑150 (MQ=255)
      ttGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCCACTGGGGGAGGGCTGTTTTGCCCGGGAGACGCCCTTGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcg                                                                                                                                            <  1:1148685/150‑1 (MQ=255)
        gCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcggg                                                                                                                                          <  1:1898572/150‑1 (MQ=255)
        gCATACAGGCCATCCTCCGGTTACAGCTTATTGCCGGGCGTCCGATCGAGGCAAGCGGGGTCAAGGTACGCCTGAGCGGGGAGGGCGGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCGCGATGCcgggcggg                                                                                                                                          <  2:412420/150‑1 (MQ=255)
         cATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGTAGAACGGCCAAGTGCTCGATGCCGGGCgggg                                                                                                                                         <  2:1890853/150‑1 (MQ=255)
          aTACAGGCCATCTCCCGGCTATAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGc                                                                                                                                        >  1:811297/1‑150 (MQ=255)
          aTACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGc                                                                                                                                        >  2:1160667/1‑150 (MQ=255)
              aGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCgggg                                                                                                                                         <  1:1420622/145‑1 (MQ=255)
              aGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCgggg                                                                                                                                         >  2:1420622/1‑145 (MQ=255)
                gCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGccc                                                                                                                                  >  2:1793304/1‑150 (MQ=255)
                gCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGccc                                                                                                                                  >  1:1995319/1‑150 (MQ=255)
                gCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGAGTCCGGGCGGGGCCTGccc                                                                                                                                  >  2:2240025/1‑150 (MQ=255)
                   aTCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGATGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCcccgcccggc                                                                                                                               >  2:913819/1‑148 (MQ=255)
                   aTCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGcc                                                                                                                               <  1:986195/150‑1 (MQ=255)
                      tcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                            >  2:2378365/1‑150 (MQ=255)
                      tcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                            <  1:2823517/150‑1 (MQ=255)
                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGATGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                          <  1:913819/150‑1 (MQ=255)
                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                          <  1:324638/150‑1 (MQ=255)
                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGccc                                                                                                                                  >  2:2069593/1‑142 (MQ=255)
                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGccc                                                                                                                                  <  1:2069593/142‑1 (MQ=255)
                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                          <  1:2378365/150‑1 (MQ=255)
                         cGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAg                                                                                                                         >  1:2513161/1‑150 (MQ=255)
                          ggCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGc                                                                                                                        <  2:964298/150‑1 (MQ=255)
                           gCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCa                                                                                                                       >  2:413753/1‑150 (MQ=255)
                              aCAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGAt                                                                                                                    <  2:2587090/150‑1 (MQ=255)
                                 gCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                          <  2:1356770/141‑1 (MQ=255)
                                     aTTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAg                                                                                                             <  2:1907352/150‑1 (MQ=255)
                                      ttGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGccc                                                                                                                                  >  2:49723/1‑128 (MQ=255)
                                      ttGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGccc                                                                                                                                  <  1:49723/128‑1 (MQ=255)
                                          cAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGAt                                                                                                        <  1:893924/150‑1 (MQ=255)
                                           aGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATc                                                                                                       <  2:2407171/150‑1 (MQ=255)
                                           aGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATc                                                                                                       >  2:2570367/1‑150 (MQ=255)
                                           aGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATc                                                                                                       <  2:811297/150‑1 (MQ=255)
                                                tCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCgggg                                                                                                                                         <  2:1915197/111‑1 (MQ=255)
                                                tCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCgggg                                                                                                                                         >  1:1915197/1‑111 (MQ=255)
                                                   gATCGAGGCAAGCTGGGTCAAGGTACGGCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCATGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCgggg                                                                                                                                         >  1:2519958/1‑108 (MQ=255)
                                                    aTCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAg                                                                                              <  1:692542/150‑1 (MQ=255)
                                                     tCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGc                                                                                             <  2:822479/150‑1 (MQ=255)
                                                       gAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCa                                                                                                                  <  2:1687001/127‑1 (MQ=255)
                                                       gAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAAAACGGGTCAGGGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGt                                                                                           >  1:1726399/1‑150 (MQ=255)
                                                          gCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATc                                                                                        >  2:2515332/1‑150 (MQ=255)
                                                            aaGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAgg                                                                                                                <  1:1602491/124‑1 (MQ=255)
                                                            aaGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGa                                                                                      >  1:2206130/1‑150 (MQ=255)
                                                               cTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGACACGCTTTTTCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGTGCGGTACCCTGGTCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGt                                                                                   <  1:2383614/150‑1 (MQ=255)
                                                                  ggTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                <  2:2857424/150‑1 (MQ=255)
                                                                   gTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGc                                                                                                                                                  >  1:1083965/1‑83 (MQ=255)
                                                                    tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                          <  1:2206688/106‑1 (MQ=37)
                                                                    tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                          >  2:2206688/1‑106 (MQ=37)
                                                                    tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtga                                                                              <  2:2513161/150‑1 (MQ=255)
                                                                    tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGACCGGTGCCCAGCGTATCGAGGTTgtga                                                                              <  1:415104/150‑1 (MQ=255)
                                                                      aaGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagt                                                                            >  2:1598323/1‑150 (MQ=255)
                                                                           aCGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCa                                                                       <  2:1148218/150‑1 (MQ=255)
                                                                           aCGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCa                                                                       <  1:16862/150‑1 (MQ=255)
                                                                              ccTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACgg                                                                    >  1:23956/1‑150 (MQ=255)
                                                                              ccTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCAGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACgg                                                                    >  2:2035038/1‑150 (MQ=255)
                                                                               cTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGcc                                                                                                                                   >  2:940174/1‑86 (MQ=255)
                                                                                 gAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGa                                                                 <  1:1598323/150‑1 (MQ=255)
                                                                                   gCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAgg                                                               <  2:1995319/150‑1 (MQ=255)
                                                                                         aGGGCTGGTCTACCCGTGAGATGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACg                                                         >  2:2505942/1‑150 (MQ=255)
                                                                                                   tACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAg                                                                                     >  1:2780749/1‑112 (MQ=255)
                                                                                                   tACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAg                                                                                     <  2:2780749/112‑1 (MQ=255)
                                                                                                   tACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCtt                                               >  1:1462585/1‑150 (MQ=255)
                                                                                                     cccGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCg                                             >  1:2670394/1‑150 (MQ=255)
                                                                                                      ccGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                <  2:871542/114‑1 (MQ=255)
                                                                                                      ccGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGa                                            >  2:151675/1‑150 (MQ=255)
                                                                                                       cGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCa                                                                                                                  >  1:2722327/1‑79 (MQ=255)
                                                                                                       cGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCa                                                                                                                  <  2:2722327/79‑1 (MQ=255)
                                                                                                       cGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCAGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGAt                                           <  1:2035038/150‑1 (MQ=255)
                                                                                                         tGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGc                                                                                                                                <  2:2757081/63‑1 (MQ=255)
                                                                                                         tGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGATTGATCACGGCGAGGTCGACGGGCTGACCTTCGATgc                                         <  1:1160667/150‑1 (MQ=255)
                                                                                                             aCGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCaa                                     >  1:2860308/1‑150 (MQ=255)
                                                                                                             aCGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCCGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCaa                                     <  2:2206130/150‑1 (MQ=255)
                                                                                                                 ccTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGAGCac                                                                                                                      <  1:112588/65‑2 (MQ=14)
                                                                                                                 ccTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGAGCac                                                                                                                      >  2:112588/1‑64 (MQ=14)
                                                                                                                 ccTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCTATGCGCAAGGcc                                 >  2:331367/1‑150 (MQ=255)
                                                                                                                     gCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGc                             >  2:422521/1‑150 (MQ=255)
                                                                                                                         gTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGccccc                         <  1:1793304/150‑1 (MQ=255)
                                                                                                                             tGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGa                     <  2:23956/150‑1 (MQ=255)
                                                                                                                               gagaACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGAt                                           <  2:270096/126‑1 (MQ=255)
                                                                                                                               gagaACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGAt                                           >  1:270096/1‑126 (MQ=255)
                                                                                                                                agaACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAg                                                                                              >  2:419115/1‑74 (MQ=255)
                                                                                                                                agaACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAg                                                                                              <  1:419115/74‑1 (MQ=255)
                                                                                                                                    cGGCCAAGTGCTCGATGCCGGGCGGGGCNNGCCCGCCATCGAGCNGNTNAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATTACGGCGAGGTCGACGGGCTGACCTTGGATGCGCAGGGCCTGGGCCCCTTGTTCAGCCg              >  1:2230040/1‑150 (MQ=255)
                                                                                                                                    cGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTg                               >  2:1035816/1‑133 (MQ=255)
                                                                                                                                    cGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTg                               <  1:1035816/133‑1 (MQ=255)
                                                                                                                                     ggCCCAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATAGGTGCCCAGCGTATCGACGTCGCGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCgg             <  1:1751174/150‑1 (MQ=255)
                                                                                                                                     ggCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATc                                                                                        >  2:2675552/1‑75 (MQ=255)
                                                                                                                                     ggCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATc                                                                                        <  1:2675552/75‑1 (MQ=255)
                                                                                                                                     cctgatctGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCgg             >  1:93431/8‑150 (MQ=38)
                                                                                                                                           gTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGa       >  1:2723283/1‑150 (MQ=255)
                                                                                                                                             gCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGcc               >  1:1286307/1‑140 (MQ=255)
                                                                                                                                             gCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGcc               <  2:1286307/140‑1 (MQ=255)
                                                                                                                                               tCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagtga                                                                          <  2:718967/79‑1 (MQ=255)
                                                                                                                                               tCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagtga                                                                          >  1:718967/1‑79 (MQ=255)
                                                                                                                                               tCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGca   <  1:2515332/150‑1 (MQ=255)
                                                                                                                                               tCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGca   >  1:2654665/1‑150 (MQ=255)
                                                                                                                                                cGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGcac  <  1:331367/150‑1 (MQ=255)
                                                                                                                                                 gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTc                                              <  1:94202/105‑1 (MQ=255)
                                                                                                                                                  aTGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGAt                                           >  1:1512125/1‑107 (MQ=38)
                                                                                                                                                     |                                                                                                                                                
AATCAGTTGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCAC  >  NC_002947/3001709‑3002002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑