Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,378,190 G→C 6.1% E66Q (GAG→CAG)  PP_2978 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,378,1900GC 6.1% 311.1 / 8.1 98E66Q (GAG→CAG) PP_2978hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (47/45);  new base C (4/2);  total (51/47)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CAAAGATTTGGAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGATGTATCACG  >  NC_002947/3378042‑3378339
                                                                                                                                                      |                                                                                                                                                     
cAAAGATTTGGAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGa                                                                                                                                                      >  2:1468771/1‑150 (MQ=255)
cAAAGATTTGGAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGa                                                                                                                                                      >  2:452399/1‑150 (MQ=255)
 aaaGATTTGGAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAg                                                                                                                                                     >  1:2314660/1‑150 (MQ=255)
  aaGATTTGGAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGc                                                                                                                                                    >  1:770970/1‑150 (MQ=255)
  aaGATTTGGAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGACAAGCACCCAACATCAGGTT‑GTTCCAGATTTCCCGCCTGAACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGc                                                                                                                                                    <  1:1596712/150‑1 (MQ=255)
   aGATTTGGAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGAGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCa                                                                                                                                                   <  2:2913991/150‑1 (MQ=255)
          gAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGcccc                                                                                                                                            >  2:1578013/1‑150 (MQ=255)
          gAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGcccc                                                                                                                                            >  2:2453512/1‑150 (MQ=255)
          gAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGcccc                                                                                                                                            >  1:2194560/1‑150 (MQ=255)
            gCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGt                                                                                                                                          <  2:1859648/150‑1 (MQ=255)
             cGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTg                                                                                                                                         <  2:392506/150‑1 (MQ=255)
             cGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTg                                                                                                                                         <  2:1368061/150‑1 (MQ=255)
              gTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCGGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTgg                                                                                                                                        >  1:2263211/1‑150 (MQ=255)
              gTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTgg                                                                                                                                        <  2:1943054/150‑1 (MQ=255)
                   gAAAGGCATGATGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGtg                                                                                                                                   <  1:1666768/150‑1 (MQ=255)
                   gAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCTGCGCTGAGCACCGCCCCGTGGTAGtg                                                                                                                                   >  2:2598574/1‑150 (MQ=255)
                     aaGGAATGGCTAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGtgat                                                                                                                                 <  1:519510/150‑1 (MQ=255)
                     aaGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGGAAGATAGTGCCACAGGCAGCGCAGTCAGCATCCCTTTCGAGCGAATTGTCGAGCGT‑AACGTCAAAGTCCGTTGTGCTG‑GCGGCGCTGGGCACCGCCCCGTGGTAGtgat                                                                                                                                 <  2:498012/150‑1 (MQ=255)
                        gAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGaa                                                                                                                              <  2:770970/150‑1 (MQ=255)
                           tGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGT‑CTGAACTGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGAt                                                                                                                           >  1:2464838/1‑150 (MQ=255)
                            ggCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGAtg                                                                                                                          <  1:298370/150‑1 (MQ=255)
                                      gggTGCGTTTCAAAGTTGAAGTGAAGGACAGTGACACGGGCAGAGCAGCCAGCATCCCTTTTGCGCGAAGGGTCGCGCGT‑CACGTCAAAGTACGTGCAGCTG‑TCGGCGCTGAGCACCGCCCCGCGGTAGTGATGAAGATGTGCATTGGCa                                                                                                                >  2:1105326/1‑150 (MQ=255)
                                       ggTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTg                                                                                                                                         >  2:2482267/1‑124 (MQ=255)
                                       ggTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTg                                                                                                                                         <  1:2482267/124‑1 (MQ=255)
                                       ggTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCaa                                                                                                               <  2:2263211/150‑1 (MQ=255)
                                       ggTGCGTTTCAAAGCTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGTATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCaa                                                                                                               <  1:2606780/150‑1 (MQ=255)
                                        gTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAAc                                                                                                              >  1:2040250/1‑150 (MQ=255)
                                        gTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAAc                                                                                                              <  2:2131694/150‑1 (MQ=255)
                                         tGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGAtgt                                                                                                                         >  1:748473/1‑138 (MQ=255)
                                         tGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGAtgt                                                                                                                         <  2:748473/138‑1 (MQ=255)
                                          gCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGtga                                                                                                                                  <  1:2121180/128‑1 (MQ=255)
                                          gCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGtga                                                                                                                                  >  2:2121180/1‑128 (MQ=255)
                                             tttCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCt                                                                                                         <  2:2314660/150‑1 (MQ=255)
                                                 aaaGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTa                                                                                                     <  1:180391/150‑1 (MQ=255)
                                                     ttGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAAc                                                                                                 <  1:911700/150‑1 (MQ=255)
                                                        aaGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGaa                                                                                              <  2:1639858/150‑1 (MQ=255)
                                                           tGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACaa                                                                                           >  1:2870013/1‑150 (MQ=255)
                                                               aGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATtct                                                                                       >  1:164195/1‑150 (MQ=255)
                                                                 aCAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTcc                                                                                     <  1:1468771/150‑1 (MQ=255)
                                                                 aCAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTcc                                                                                     >  2:1908246/1‑150 (MQ=255)
                                                                  cAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTccc                                                                                    <  1:656624/150‑1 (MQ=255)
                                                                   aGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCt                                                                                   >  2:2750904/1‑150 (MQ=255)
                                                                    gTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGTGCTCAGCgccgcc                                                                                                                                              >  2:1146890/1‑90 (MQ=255)
                                                                    gTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGTGCTCAGCgccgcc                                                                                                                                              <  1:1146890/90‑1 (MQ=255)
                                                                      gACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCTGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTgaa                                                                                <  1:2598574/150‑1 (MQ=255)
                                                                         acaGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCTGCGCTGAGCACCGACCCGCGGTAGTGCAGAAGATGTGCATTGGGAACCAGCTTTTACAACGAAAAATTTTCCCTgcggca                                                                             >  2:492308/1‑145 (MQ=255)
                                                                           aGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTgg                                                                                                                                        <  1:765835/89‑1 (MQ=255)
                                                                           aGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTgg                                                                                                                                        >  2:765835/1‑89 (MQ=255)
                                                                            ggCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCg                                                                          <  1:2453512/150‑1 (MQ=255)
                                                                            ggCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCg                                                                          >  2:2140906/1‑150 (MQ=255)
                                                                             gCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCgg                                                                         <  1:1578013/150‑1 (MQ=255)
                                                                               agagCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTCCTG‑GCGGCGCTGAGCACCGCCCCGTGGGAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGTGa                                                                       >  2:2779240/1‑150 (MQ=255)
                                                                                       cagcATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTTAATAACCGGGAAAAAATGc                                                               <  2:2040250/150‑1 (MQ=255)
                                                                                        agcATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCt                                                              >  1:268028/1‑150 (MQ=255)
                                                                                             cccTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTcc                                                                                     <  1:2552407/122‑1 (MQ=255)
                                                                                             cccTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTcc                                                                                     >  2:2552407/1‑122 (MQ=255)
                                                                                             cccTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATc                                                         >  2:1600777/1‑150 (MQ=255)
                                                                                               cTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCa                                                                                                            >  1:3042569/1‑97 (MQ=255)
                                                                                               cTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCa                                                                                                            <  2:3042569/97‑1 (MQ=255)
                                                                                               cTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCg                                                       >  1:903865/1‑150 (MQ=255)
                                                                                               cTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCg                                                       >  2:316294/1‑150 (MQ=255)
                                                                                                tttCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGtg                                                                                                                                   >  2:2242797/1‑73 (MQ=255)
                                                                                                tttCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGtg                                                                                                                                   <  1:2242797/73‑1 (MQ=255)
                                                                                                tttCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCgg                                                      >  1:2627932/1‑150 (MQ=255)
                                                                                                tttCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCgg                                                      >  2:1949518/1‑150 (MQ=255)
                                                                                                  tCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGtt                                                    <  1:1908246/150‑1 (MQ=255)
                                                                                                           gggTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCgg                                                      >  1:3032342/1‑139 (MQ=255)
                                                                                                           gggTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCgg                                                      <  2:3032342/139‑1 (MQ=255)
                                                                                                              tCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGt                                        >  2:2922680/1‑150 (MQ=255)
                                                                                                              tCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGt                                        >  1:1877112/1‑150 (MQ=255)
                                                                                                                          gTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCGCTGGa                             >  1:642973/1‑150 (MQ=255)
                                                                                                                          gTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGa                             >  2:1590762/1‑150 (MQ=255)
                                                                                                                          gTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCAGGGTTTGATCGGCCGTCGAGCACTGGa                             >  2:93049/1‑150 (MQ=255)
                                                                                                                           tCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCACTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAg                            >  2:555210/1‑150 (MQ=255)
                                                                                                                                gTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCt                       >  2:1804880/1‑150 (MQ=255)
                                                                                                                                gTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCt                       >  2:914274/1‑150 (MQ=255)
                                                                                                                                 tCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGa                             <  1:876550/143‑1 (MQ=255)
                                                                                                                                 tCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGa                             >  2:876550/1‑143 (MQ=255)
                                                                                                                                  ccGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAg                     <  2:164195/150‑1 (MQ=255)
                                                                                                                                  ccGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAg                     <  2:2627932/150‑1 (MQ=255)
                                                                                                                                    gtggtgCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTcc                                                                                     <  2:1913454/84‑1 (MQ=255)
                                                                                                                                    gtggtgCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTcc                                                                                     >  1:1913454/1‑84 (MQ=255)
                                                                                                                                     tggtgCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTa                      <  2:945995/146‑1 (MQ=255)
                                                                                                                                     tggtgCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTa                      >  1:945995/1‑146 (MQ=255)
                                                                                                                                     accacggg‑gcggtGCTCAGCGCCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAg                  >  1:274089/8‑150 (MQ=255)
                                                                                                                                      ccacggg‑gcggtGCTCAGCGCCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGc                 >  2:1542690/7‑150 (MQ=255)
                                                                                                                                       gtgCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCg                <  1:1600777/150‑1 (MQ=255)
                                                                                                                                        tgCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGa                             <  2:1118119/136‑1 (MQ=255)
                                                                                                                                        tgCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGa                             >  1:1118119/1‑136 (MQ=255)
                                                                                                                                           tG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTgg            <  2:903865/150‑1 (MQ=255)
                                                                                                                                            g‑gcggtGCTCAGCGCCGCCCCGTGGTAGTGATTAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGa           <  1:1542690/150‑1 (MQ=255)
                                                                                                                                            g‑gcggcGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGa           <  2:2870013/150‑1 (MQ=255)
                                                                                                                                              gcggtgCTCAGCGCCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGAt          >  1:2753802/1‑150 (MQ=255)
                                                                                                                                                ggcgCTGAGCACCGCCCCGTGGTAGGGATGCAGCTGGGCACTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGATGt        <  1:1105326/150‑1 (MQ=255)
                                                                                                                                                   gcTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGATGTATc     >  1:1158534/1‑150 (MQ=255)
                                                                                                                                                    cTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGATGTATCa    <  2:2194560/150‑1 (MQ=255)
                                                                                                                                                     tGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGATGTATCAc   >  2:2896031/1‑150 (MQ=255)
                                                                                                                                                     tGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGATGTATCAc   <  1:452399/150‑1 (MQ=255)
                                                                                                                                                      gAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGATGTATCACg  >  2:1546398/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CAAAGATTTGGAGCGTTTCGAAAGGAATGGCGAGAAATGGGTGCGTTTCAAAGTTGAAGTGAAAGACAGTGACACAGGCAGAGCAGCCAGCATCCCTTTCGAGCGAAGGGTCGAGCGT‑AACGTCAAAGTCCGTGGTGCTG‑GCGGCGCTGAGCACCGCCCCGTGGTAGTGATGAAGATGTGCATTGGCAACCAGCTTTTACAACGAACAATTCTCCCTGAAGAACCGGGAAAAAATGCTCCATCCGGTTTTGATCGGCCGTCGAGCACTGGAGCACCTAGGAGCGGTGGATGTATCACG  >  NC_002947/3378042‑3378339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑