Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 308,740 C→T 11.5% intergenic (+1754/‑116) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947308,7400CT11.5% 270.6 / 20.2 104intergenic (+1754/‑116)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (41/51);  new base T (8/4);  total (49/55)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 108 total aligned reads displayed.

TGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCTCCC  >  NC_002947/308596‑308889
                                                                                                                                                |                                                                                                                                                     
tGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAgggg                                                                                                                                                  <  1:398264/150‑1 (MQ=255)
          gCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCTGGGGGCGGCtttctc                                                                                                                                        <  2:2115123/150‑4 (MQ=255)
          gCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCtttttt                                                                                                                                        >  2:813877/1‑150 (MQ=255)
             tACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTACGCCGAGCTCAGCCGCAACGAGAAAGCCGCCACCAGGGGCGGCTTTTTtgtg                                                                                                                                     <  2:431860/150‑1 (MQ=255)
             tACGACGAGGCTGCCAAAGGTCTGGACAAGCTGTTCATCGAAAAGCTGAATAAGTTCTAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgtg                                                                                                                                     <  2:859080/150‑1 (MQ=255)
              acgacgAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                                    <  2:109249/150‑1 (MQ=255)
              acgacgAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                                    <  2:2153060/150‑1 (MQ=255)
              acgacgAGGCTGCCAAAGCTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCCAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCTGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                                    <  2:1369077/150‑1 (MQ=255)
                gacgaGTCTCCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGt                                                                                                                                  >  2:1531038/1‑150 (MQ=255)
                 acgaGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGtt                                                                                                                                 <  2:1541873/150‑1 (MQ=255)
                    aGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAgg                                                                                                                              <  1:813877/150‑1 (MQ=255)
                    aGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAgg                                                                                                                              <  2:461684/150‑1 (MQ=255)
                        tGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGTGGTAGGGCtt                                                                                                                          >  1:472238/1‑150 (MQ=255)
                        tGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAgg                                                                                                                              >  1:1062170/1‑146 (MQ=255)
                         gCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTg                                                                                                                         >  2:377822/1‑150 (MQ=255)
                          ccAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAg                                                                                                                               >  1:901990/1‑143 (MQ=255)
                          ccAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAg                                                                                                                               <  2:901990/143‑1 (MQ=255)
                           cAAAGGTCTGGCCAAAATGTTCATCAAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGcc                                                                                                                       >  2:1723162/1‑150 (MQ=255)
                                       cAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgt                                                                                                                                      <  2:1241899/123‑1 (MQ=255)
                                       cAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgt                                                                                                                                      >  1:1241899/1‑123 (MQ=255)
                                        aaGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGccc                                                                                                          <  2:589118/150‑1 (MQ=255)
                                        aaGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGccc                                                                                                          <  1:1198122/150‑1 (MQ=255)
                                                  tCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGAAg                                                                                                <  1:561053/150‑1 (MQ=255)
                                                    gAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCg                                                                                              >  1:2550688/1‑150 (MQ=255)
                                                      aaaCTTCAAGAAGTTCGAAGTTTCCCACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGaa                                                                                            <  2:240118/150‑1 (MQ=255)
                                                          ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTc                                                                                        >  1:1879179/1‑150 (MQ=255)
                                                          ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgtg                                                                                                                                     <  2:1361983/105‑1 (MQ=255)
                                                          ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgtg                                                                                                                                     >  1:1361983/1‑105 (MQ=255)
                                                          ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAg                                                                                                >  2:847804/1‑142 (MQ=255)
                                                          ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAg                                                                                                <  1:847804/142‑1 (MQ=255)
                                                           tCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCt                                                                                       <  2:1669906/150‑1 (MQ=255)
                                                             aagaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgt                                                                                                                                      >  1:379160/1‑101 (MQ=255)
                                                             aagaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTgg                                                                                     >  2:1275873/1‑150 (MQ=255)
                                                              agaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGt                                                                                    >  1:1198970/1‑150 (MQ=255)
                                                                  gTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttttt                                                                                <  2:1198970/150‑1 (MQ=255)
                                                                  gTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCAGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttttt                                                                                <  2:1346220/150‑1 (MQ=255)
                                                                   ttCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAg                                                                                                                               <  2:2460368/102‑1 (MQ=255)
                                                                   ttCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAg                                                                                                                               >  1:2460368/1‑102 (MQ=255)
                                                                   ttCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTc                                                                               >  1:703341/1‑150 (MQ=255)
                                                                   ttCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGAAGGGAAAGTCTGGTTTTTc                                                                               >  2:2698468/1‑150 (MQ=255)
                                                                    tCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCt                                                                              <  1:1509311/150‑1 (MQ=255)
                                                                      gAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGAAACGAGATGTCGGCCGCCAGGGGAGGCTTTTTTGTGGGTTAGGGTTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGt                                                                            <  1:2993548/150‑1 (MQ=255)
                                                                          tttCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCa                                                                        <  1:1275873/150‑1 (MQ=255)
                                                                           ttCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGc                                                                                                            <  1:23914/113‑1 (MQ=255)
                                                                           ttCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGc                                                                                                            >  2:23914/1‑113 (MQ=255)
                                                                            tCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTACAACTGGCGCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACt                                                                      <  1:30688/150‑1 (MQ=255)
                                                                            tCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACt                                                                      <  2:2532356/150‑1 (MQ=255)
                                                                            tCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACt                                                                      <  1:304656/150‑1 (MQ=255)
                                                                                    cATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGACGGGGCCCGCATAGGCAGCGAAAGTCTGGTATTTCAGTTTCCCTGTCAGCAt                                                              >  2:838301/1‑150 (MQ=255)
                                                                                    cATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCAt                                                              >  2:2656481/1‑150 (MQ=255)
                                                                                     aTCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAg                                                                                                                >  2:947376/1‑99 (MQ=255)
                                                                                     aTCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAg                                                                                                                <  1:947376/99‑1 (MQ=255)
                                                                                     aTCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATc                                                             >  1:1928448/1‑150 (MQ=255)
                                                                                      tCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCa                                                                                                 >  1:2190937/1‑113 (MQ=255)
                                                                                      tCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCa                                                                                                 <  2:2190937/113‑1 (MQ=255)
                                                                                      tCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTCTGCTGGGTAGGGCTTGCCCGCGACGGGGACCGGGCAGACAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATcc                                                            >  1:689313/1‑150 (MQ=255)
                                                                                        aaGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAt                                                          <  2:1928448/150‑1 (MQ=255)
                                                                                         aGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCg                                                                                                         <  2:343264/102‑1 (MQ=255)
                                                                                         aGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCg                                                                                                         >  1:343264/1‑102 (MQ=255)
                                                                                           gCCGCTGGCCCGCAGCTGTACGCCGATCCCAGCTGCAACGAGAAAGCCGCCCCCCGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGc                                                       >  1:2374328/1‑150 (MQ=255)
                                                                                           gCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTg                                                                                      <  1:419541/119‑1 (MQ=255)
                                                                                           gCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTg                                                                                      >  2:419541/1‑119 (MQ=255)
                                                                                             cGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAg                                                                                                >  1:961590/1‑107 (MQ=255)
                                                                                                tGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGa                                                  <  2:1752997/150‑1 (MQ=255)
                                                                                                      gCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAagag                                            >  2:306751/1‑150 (MQ=255)
                                                                                                           tGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagccagc                                       >  1:1450066/1‑150 (MQ=255)
                                                                                                             tAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGaaa                                                                                           >  1:2451221/1‑96 (MQ=255)
                                                                                                               aGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGa                                   <  1:1723162/150‑1 (MQ=255)
                                                                                                                   gAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGAc                               <  1:306751/150‑1 (MQ=255)
                                                                                                                       tCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGaa                           <  2:689313/150‑1 (MQ=255)
                                                                                                                        cAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAg                          <  1:377822/150‑1 (MQ=255)
                                                                                                                           ccGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagcca                                         >  1:1712960/1‑132 (MQ=255)
                                                                                                                           ccGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagcca                                         <  2:1712960/132‑1 (MQ=255)
                                                                                                                              cAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGTGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGaaa                                                                                           <  1:2121711/79‑1 (MQ=255)
                                                                                                                              cAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGaaa                                                                                           >  2:2121711/1‑79 (MQ=255)
                                                                                                                               aaCGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGt                                                                    >  1:326483/1‑101 (MQ=255)
                                                                                                                               aaCGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGt                                                                    <  2:326483/101‑1 (MQ=255)
                                                                                                                               aaCGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATcc                                                            <  2:2461453/109‑1 (MQ=255)
                                                                                                                               aaCGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATcc                                                            >  1:2461453/1‑109 (MQ=255)
                                                                                                                               aaCGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCa                   <  2:1450066/150‑1 (MQ=255)
                                                                                                                                acaaaaaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGAGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAg                  >  2:1534331/6‑150 (MQ=255)
                                                                                                                                  aaaaaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAgcg                >  1:400959/4‑150 (MQ=255)
                                                                                                                                   agaAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttt                                                                                  >  1:1292789/1‑83 (MQ=255)
                                                                                                                                   agaAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttt                                                                                  <  2:1292789/83‑1 (MQ=255)
                                                                                                                                   agaAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAgcgc               >  2:2374111/1‑150 (MQ=255)
                                                                                                                                   aaaaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGttt                                                                          >  1:362682/3‑91 (MQ=39)
                                                                                                                                   aaaaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGttt                                                                          <  2:362682/89‑1 (MQ=39)
                                                                                                                                     aaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTATGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGGCAGGCACATGACCGAAGCGCCGCAGCGCAc             >  1:1078723/1‑150 (MQ=255)
                                                                                                                                     aaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGAc                                                 <  2:355916/114‑1 (MQ=255)
                                                                                                                                     aaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGAc                                                 >  1:355916/1‑114 (MQ=255)
                                                                                                                                     aaaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCAc             >  2:2828247/1‑150 (MQ=255)
                                                                                                                                       aGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTg           >  1:1285713/1‑150 (MQ=255)
                                                                                                                                        gccgccCCTGGGGGCGGCTTTTTTGTGGGTCAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAtt                                                         <  1:197126/103‑1 (MQ=255)
                                                                                                                                        gccgccCCTGGGGGCGGCTTTTTTGTGGGTCAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAtt                                                         >  2:197126/1‑103 (MQ=255)
                                                                                                                                         ccgccCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGcc         >  1:639557/1‑150 (MQ=255)
                                                                                                                                         ccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCACAGGCAGCGAAAGTCTGGTTGTTCTGTTTCACTGTCCGCAGCCACCACGACGACCACAACAAGCCAGCTGACCGAAGCCCCTCAGCGCACTGcc         <  1:2374111/150‑1 (MQ=255)
                                                                                                                                            cccccAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTtc      <  2:2935150/150‑1 (MQ=255)
                                                                                                                                              cccAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTtctc    >  1:2618770/1‑150 (MQ=255)
                                                                                                                                               ccAGGGGCGGCTTTTTTGGGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCTcc   <  2:472238/150‑1 (MQ=255)
                                                                                                                                                cAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGAACTGACTtctcac  >  1:2051858/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCTCCC  >  NC_002947/308596‑308889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑