Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,800,887 C→T 7.8% intergenic (+54/+25) PP_3360 → / ← PP_3361 membrane protein/aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,800,8870CT 7.8% 217.2 / 7.1 77intergenic (+54/+25)PP_3360/PP_3361membrane protein/aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (36/35);  new base T (4/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GTGCCGGTGCGCGCGATCAAGGTCACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCTTCGGTGCCGAT  >  NC_002947/3800741‑3801034
                                                                                                                                                  |                                                                                                                                                   
gTGCCGGTGCGCGCGATCAAGGTCACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCACGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCa                                                                                                                                                  <  2:1504405/150‑1 (MQ=255)
   ccGGTGCGCGCGATCAAGGTCACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGc                                                                                                                                               >  1:416579/1‑150 (MQ=255)
   ccGGTGCGCGCGATCAAGGTCACGACATTGCCAGAGGCGTCCGGTCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGCGGCGCTTGATGATGGCATCCTGCCCGGCTCGCCTGATAGGTGCACCGCAGGGCGTGCACATGCGCACgg                                                                                                                                               <  2:1231828/150‑2 (MQ=255)
        gcgcgcGATCAAGGTCACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGc                                                                                                                                          <  2:716381/150‑1 (MQ=255)
        gcgcgcGATCAAGGTCACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGc                                                                                                                                          <  2:3028565/150‑1 (MQ=255)
            gcgATCAAGGTCACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCcgc                                                                                                                                      >  1:16230/1‑150 (MQ=255)
                tCAAGGTCACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGACCGCGCTg                                                                                                                                  >  1:909517/1‑150 (MQ=255)
                     gTCACGGCATTGACAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACg                                                                                                                             >  2:1573154/1‑150 (MQ=255)
                      tCACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCAAGCCCTGCCTCCGCTGaagaaa                                                                                                                            >  2:2952445/1‑146 (MQ=255)
                       cACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCt                                                                                                                           >  2:1213599/1‑150 (MQ=255)
                       cACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCt                                                                                                                           <  2:1231373/150‑1 (MQ=255)
                         cGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTAt                                                                                                                         >  1:2503293/1‑150 (MQ=255)
                          ggCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTAtt                                                                                                                        >  2:469219/1‑150 (MQ=255)
                           gCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTAttt                                                                                                                       <  1:2313229/150‑1 (MQ=255)
                             aTTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCGCATGTGCACGCCCt                                                                                                                                            >  2:1187547/1‑127 (MQ=255)
                             aTTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCGCATGTGCACGCCCt                                                                                                                                            <  1:1187547/127‑1 (MQ=255)
                               tGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACTTGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAgc                                                                                                                   <  1:593615/150‑1 (MQ=255)
                                gCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTc                                                                                                                      >  2:730419/1‑146 (MQ=255)
                                gCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTc                                                                                                                      <  1:730419/146‑1 (MQ=255)
                                  cAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGcgcc                                                                                                                >  1:1352579/1‑150 (MQ=255)
                                  cAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGcgcc                                                                                                                >  1:2054143/1‑150 (MQ=255)
                                     agGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCcgcg                                                                                                             >  2:2678010/1‑150 (MQ=255)
                                     agGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCcgcg                                                                                                             <  2:1700777/150‑1 (MQ=255)
                                     agGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCCGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGc                                                                                                                                               >  2:694637/1‑116 (MQ=255)
                                     agGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCCGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGc                                                                                                                                               <  1:694637/116‑1 (MQ=255)
                                           ccGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAg                                                                                                       <  2:1352579/150‑1 (MQ=255)
                                            cGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGc                                                                                                      >  1:1615377/1‑150 (MQ=255)
                                               ccTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGt                                                                                                   >  1:556327/1‑150 (MQ=255)
                                                cTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTc                                                                                                  >  2:2341290/1‑150 (MQ=255)
                                                   cATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAg                                                                                                       >  2:1705262/1‑142 (MQ=255)
                                                   cATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAg                                                                                                       <  1:1705262/142‑1 (MQ=255)
                                                    gtGGCCTGGCTGGCCAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCCCATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTc                                                                                              <  2:655335/149‑1 (MQ=255)
                                                    aTGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGTCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTc                                                                                              <  1:232068/150‑1 (MQ=255)
                                                    aTGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTc                                                                                              <  2:2503293/150‑1 (MQ=255)
                                                    aTGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTc                                                                                              >  2:1182166/1‑150 (MQ=255)
                                                                 cgAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCaagaa                                                                                 <  1:469219/150‑1 (MQ=255)
                                                                  gAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCaagaag                                                                                <  1:2976868/150‑1 (MQ=255)
                                                                     ggCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAgaaggaa                                                                             <  2:16230/150‑1 (MQ=255)
                                                                     ggCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCACATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCAGAAATTCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAgaaggaa                                                                             <  1:1616236/150‑1 (MQ=255)
                                                                        cTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCGCATGTGCACGCCCTGc                                                                                                                                          >  1:1354001/1‑86 (MQ=255)
                                                                        cTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCGCATGTGCACGCCCTGc                                                                                                                                          <  2:1354001/86‑1 (MQ=255)
                                                                                     gcgcTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCACGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGTACCTCAAGAAGGAAGTCCGCATTGGCCCgg                                                             <  2:2054143/150‑1 (MQ=255)
                                                                                       gcTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGTCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCt                                                           <  2:1516729/150‑1 (MQ=255)
                                                                                        cTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTg                                                          <  1:2341290/150‑1 (MQ=255)
                                                                                         ttGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAgaaggaag                                                                            >  1:1105320/1‑131 (MQ=255)
                                                                                         ttGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAgaaggaag                                                                            <  2:1105320/131‑1 (MQ=255)
                                                                                           gATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGt                                                                                                   <  2:2099368/106‑1 (MQ=255)
                                                                                           gATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGt                                                                                                   >  1:2099368/1‑106 (MQ=255)
                                                                                                tGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTc                                                                                              >  1:2459550/1‑106 (MQ=255)
                                                                                                tGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTc                                                                                              <  2:2459550/106‑1 (MQ=255)
                                                                                                  gCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTAttt                                                                                                                       <  1:512392/79‑1 (MQ=255)
                                                                                                  gCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTAttt                                                                                                                       >  2:512392/1‑79 (MQ=255)
                                                                                                       cTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGc                                           >  2:1511032/1‑150 (MQ=255)
                                                                                                        tGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGcc                                          >  2:817261/1‑150 (MQ=255)
                                                                                                             ggCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCCGTAATGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCAtt                                     >  1:2636770/1‑150 (MQ=255)
                                                                                                             ggCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGATATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCAAAGGCCGCAtt                                     >  1:1091588/1‑150 (MQ=255)
                                                                                                              gCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTg                                                                   <  2:1937555/119‑1 (MQ=255)
                                                                                                              gCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTg                                                                   >  1:1937555/1‑119 (MQ=255)
                                                                                                                    cAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCa                              <  2:564942/150‑1 (MQ=255)
                                                                                                                       aTAGGTGCACCGCAGGGCGTGCACATGCGCACGGCCTGCCGGCGCGGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGACCCGGCGGAAAATCATAGAAGGCCGCGTTTTCAGCACGt                           >  2:88482/1‑150 (MQ=255)
                                                                                                                           gTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAACCGCTATTTCAGCGCCGCGGTGAAGCGGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGCTGTc                       >  2:348616/1‑150 (MQ=255)
                                                                                                                           gTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTc                       >  2:582670/1‑150 (MQ=255)
                                                                                                                            tGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCa                      >  2:1969797/1‑150 (MQ=255)
                                                                                                                            tGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCa                      >  1:1438477/1‑150 (MQ=255)
                                                                                                                              cACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATg                    >  1:735540/1‑150 (MQ=255)
                                                                                                                                 cGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCgg                 <  1:2794772/150‑1 (MQ=255)
                                                                                                                                 cGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCAGACGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCgg                 <  1:1573154/150‑1 (MQ=255)
                                                                                                                                   cAGGGCGTGCGCATGTGCACGCCCTGCCCGCGCTGAAACGCTAGTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCAGCATTTTCAGCACGTTGTCATGAggga               >  2:2228882/1‑149 (MQ=255)
                                                                                                                                      ggCGTGCGCATGTGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCtt            >  1:1496102/1‑150 (MQ=255)
                                                                                                                                        cGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGCAGCTGTTGTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCTTCg          <  2:416579/150‑1 (MQ=255)
                                                                                                                                         gTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCTTCgg         >  2:2002552/1‑150 (MQ=255)
                                                                                                                                            cacaTGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGCAGGAAGTCCGCATTGGCCCGGCTTAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCTTCGGTGc      <  1:2678010/150‑1 (MQ=255)
                                                                                                                                               aTGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGc                               <  1:2168148/122‑1 (MQ=255)
                                                                                                                                               aTGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGc                               >  2:2168148/1‑122 (MQ=255)
                                                                                                                                               aTGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCTTCGGTGCCGa   <  1:817261/150‑1 (MQ=255)
                                                                                                                                                tGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCTTCGGTGCCgct  <  1:1213599/150‑3 (MQ=255)
                                                                                                                                                tGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGAACCTCAAGAAGGTCGTACGCATTGACCCTGCTGAAGGTAAACGAAGCCCGCATTGCCAGCACGTTTTCATGCGGGCCTTCGGTGCCGAt  >  2:2941422/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTGCCGGTGCGCGCGATCAAGGTCACGGCATTGCCAGAGGCGTCCGGCCTGCATGGCCAGGCTCGCGAAGGCCTGAGCATCAGGGGCGCTTGATGCTGGCAACCTGCCCGGCTCGCCAGATAGGTGCACCGCAGGGCGTGCACATGCGCACGCCCTGCCCGCGCTGAAACGCTATTTCAGCGCCGCGGTGAAGCTGTCTTTCAGCACCTCAAGAAGGAAGTCCGCATTGGCCCGGCTGAAAATCATCGAAGGCCGCATTTTCAGCACGTTGTCATGCGGGCCTTCGGTGCCGAT  >  NC_002947/3800741‑3801034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑