BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,013,339	G→A	5.3%	A38T (GCG→ACG)	PP_5678 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,013,339	0	G	A	5.3%	339.5 / 5.3	112	A38T (GCG→ACG)	PP_5678	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (51/55); new base A (3/3); total (54/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 117 total aligned reads displayed.

GCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTACTCT  >  NC_002947/5013193‑5013483

gCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGAGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTGTGATGATGGTGTACGAC‑AGCACGCCGTGCTGAGCgcgg                                                                                                                                               >  1:778778/1‑150 (MQ=255)

 cAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGCGCTCAGCacggc                                                                                                                                              <  2:363126/150‑1 (MQ=255)

       aacaaGCCTCTGACTGAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGaa                                                                                                                                        >  2:2422895/1‑150 (MQ=255)

       aacaaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGcgcg                                                                                                                                                >  2:1832096/1‑142 (MQ=255)

       aacaaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGcgcg                                                                                                                                                <  1:1832096/142‑1 (MQ=255)

        acaaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGaaa                                                                                                                                       <  1:266430/150‑1 (MQ=255)

         caaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAAt                                                                                                                                      >  2:2059921/1‑150 (MQ=255)

         caaGCCTCAGACTTAGGGGAGAGCCAATGAGGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTgcattc                                                                                                                                      <  1:1643159/150‑6 (MQ=255)

          aaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATc                                                                                                                                     >  2:116231/1‑150 (MQ=255)

          aaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATc                                                                                                                                     <  1:2026431/150‑1 (MQ=255)

           aGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCa                                                                                                                                    <  1:116231/150‑1 (MQ=255)

               tCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATTACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGACt                                                                                                                                >  2:1411449/1‑150 (MQ=255)

                   aCTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCgca                                                                                                                            <  2:3031528/150‑1 (MQ=255)

                    cTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCgcag                                                                                                                           <  1:197059/150‑1 (MQ=255)

                     ttAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAAt                                                                                                                                      <  2:746302/138‑1 (MQ=255)

                              agCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCg                                                                                                                 >  1:2189932/1‑150 (MQ=255)

                               gCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGa                                                                                                                >  2:1113704/1‑150 (MQ=255)

                               gCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGa                                                                                                                >  1:2872513/1‑150 (MQ=255)

                                ccAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑CGTAAGGCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGaa                                                                                                               <  2:431203/150‑1 (MQ=255)

                                  aaTGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGc                                                                                                             >  2:814501/1‑150 (MQ=255)

                                    tgatgaTCAAAGAGTTGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCt                                                                                                           <  2:2818381/150‑1 (MQ=255)

                                         ttCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCCGCGTAGTCTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCTGCCTGAGCGCGGCTTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGaa                                                                                                      <  2:1200617/149‑1 (MQ=255)

                                         atCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCt                                                                                                           <  2:1566083/145‑1 (MQ=255)

                                         atCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCt                                                                                                           >  1:1566083/1‑145 (MQ=255)

                                         atCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGaa                                                                                                      >  2:1811590/1‑150 (MQ=255)

                                             aaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTcc                                                                                                  >  2:381105/1‑150 (MQ=255)

                                                 gTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGGCGTTCTGATGATGGCC‑CCCACAAGCACGCCGTGCTCGGTAATTCGTTGAAGTCGATCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCt                                                                                              <  1:2363321/150‑1 (MQ=255)

                                                   cGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCttt                                                                                            <  2:829076/150‑1 (MQ=255)

                                                    gACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGaa                                                                                                               <  1:636059/130‑1 (MQ=255)

                                                     aCCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGc                                                                                          <  2:876571/150‑1 (MQ=255)

                                                     aCCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGc                                                                                          <  1:381105/150‑1 (MQ=255)

                                                     aCCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGc                                                                                          <  2:1684371/150‑1 (MQ=255)

                                                      cccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTgg                                                                                                                                          <  1:921663/101‑1 (MQ=255)

                                                      cccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTgg                                                                                                                                          >  2:921663/1‑101 (MQ=255)

                                                      cccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCg                                                                                         >  1:1037509/1‑150 (MQ=255)

                                                       ccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCga                                                                                        >  2:7084/1‑150 (MQ=255)

                                                       ccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCga                                                                                        >  1:1162710/1‑150 (MQ=255)

                                                        cTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCgag                                                                                       >  1:248481/1‑150 (MQ=255)

                                                         tCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCgaga                                                                                      <  1:1534736/150‑1 (MQ=255)

                                                         tCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCgaga                                                                                      <  1:2125333/150‑1 (MQ=255)

                                                         tCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCgaga                                                                                      >  1:662775/1‑150 (MQ=255)

                                                              gCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAgctgc                                                                                 <  2:1515843/150‑1 (MQ=255)

                                                              gCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAgctgc                                                                                 <  1:814501/150‑1 (MQ=255)

                                                                cGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGctt                                                                               <  1:1113704/150‑1 (MQ=255)

                                                                 gTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGcttc                                                                              <  1:508065/150‑1 (MQ=255)

                                                                      ggTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGc                                                                         <  2:1333363/150‑1 (MQ=255)

                                                                           gCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCCCGc                                                                    >  2:2635510/1‑150 (MQ=255)

                                                                           gCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGc                                                                    >  2:2154638/1‑150 (MQ=255)

                                                                             gTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAgctgc                                                                                 <  2:2270119/135‑1 (MQ=255)

                                                                             gTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAgctgc                                                                                 >  1:2270119/1‑135 (MQ=255)

                                                                               aGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTc                                                                >  1:194783/1‑150 (MQ=255)

                                                                                 tttGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCaa                                                              >  2:579601/1‑150 (MQ=255)

                                                                                  ttGCGAGCGTTGCGGCCAGACTTGTCCTTCTGATCACGGTCATCCAC‑AGCACGCCGTGCTGAGCGCGGCCTGGAAATCAGGCTGGCAGCAATACCTTTTAGCCTATGAACTCCATCTGTGCGAGAGCTGCTTCTTTGCGACGCTTTCAAc                                                             <  2:46754/150‑1 (MQ=255)

                                                                                   tGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCACCg                                                            >  1:682285/1‑150 (MQ=255)

                                                                                          gTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGc                                                     <  2:549244/150‑1 (MQ=255)

                                                                                           ttGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCgaga                                                                                      <  2:413247/116‑1 (MQ=255)

                                                                                            tGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGt                                                   <  2:1652023/150‑1 (MQ=255)

                                                                                             gCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTg                                                  <  1:2635510/150‑1 (MQ=255)

                                                                                               gtccATACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGaa                                                <  2:778778/148‑1 (MQ=255)

                                                                                               ggCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGc                                                     <  1:2815240/145‑1 (MQ=255)

                                                                                               ggCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGc                                                     >  2:2815240/1‑145 (MQ=255)

                                                                                                gCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAAc                                               >  2:2966619/1‑150 (MQ=255)

                                                                                                     aCTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATc                                                                                               <  1:312062/97‑1 (MQ=255)

                                                                                                     aCTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATc                                                                                               >  2:312062/1‑97 (MQ=255)

                                                                                                       ttGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGc                                                                         >  1:845371/1‑117 (MQ=255)

                                                                                                          tCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTa                                                                                                          >  2:1173700/1‑81 (MQ=255)

                                                                                                          tCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTa                                                                                                          <  1:1173700/81‑1 (MQ=255)

                                                                                                          tCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCa                                     >  1:2724235/1‑150 (MQ=255)

                                                                                                            cTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGc                                             >  2:946490/1‑140 (MQ=255)

                                                                                                            cTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGc                                             <  1:946490/140‑1 (MQ=255)

                                                                                                             ttCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGc                                                                                                                                 >  1:1972437/1‑55 (MQ=255)

                                                                                                             ttCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGc                                                                                                                                 <  2:1972437/55‑1 (MQ=255)

                                                                                                             ttCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAAtt                                  >  2:1254106/1‑150 (MQ=255)

                                                                                                                tgatgaCGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGAt                               <  1:2966619/150‑1 (MQ=255)

                                                                                                                  atgaCGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGc                                                                                          >  1:2839717/1‑89 (MQ=255)

                                                                                                                  atgaCGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGc                                                                                          <  1:1506810/89‑1 (MQ=255)

                                                                                                                  atgaCGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGc                                                                                          >  2:1506810/1‑89 (MQ=255)

                                                                                                                       ggCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAGGCGTGAACGCTGGGCCCAATTGATGTTctct                        <  2:2872513/150‑1 (MQ=255)

                                                                                                                       ggCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTctct                        >  1:463538/1‑150 (MQ=255)

                                                                                                                       ggCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTctct                        <  2:2915978/150‑1 (MQ=255)

                                                                                                                       ggCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTctct                        >  2:1264678/1‑150 (MQ=255)

                                                                                                                       ggCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTctct                        <  1:2154638/150‑1 (MQ=255)

                                                                                                                          cATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAg                     <  1:1565617/150‑1 (MQ=255)

                                                                                                                               ac‑aGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGc                >  2:911461/1‑150 (MQ=255)

                                                                                                                               ac‑aGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGc                >  2:801142/1‑150 (MQ=255)

                                                                                                                                acagaccGCCGTGCTGAGCGCGGCGTGGCAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGc                <  1:1411449/144‑1 (MQ=255)

                                                                                                                                   gCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGa             <  2:194783/150‑1 (MQ=255)

                                                                                                                                    cACGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAAtt                                  >  2:2516583/1‑128 (MQ=255)

                                                                                                                                    cACGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAAtt                                  <  1:2516583/128‑1 (MQ=255)

                                                                                                                                     aCGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCttt                                                                           <  2:977456/86‑1 (MQ=255)

                                                                                                                                     aCGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCttt                                                                           >  1:977456/1‑86 (MQ=255)

                                                                                                                                      cGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTc          <  1:1811590/150‑1 (MQ=255)

                                                                                                                                         cGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCtt       <  2:1162710/150‑1 (MQ=255)

                                                                                                                                         cGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCtt       >  1:2116433/1‑150 (MQ=255)

                                                                                                                                          gTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCCAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTa      <  1:659776/150‑1 (MQ=255)

                                                                                                                                           tGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTa      >  2:2266872/1‑149 (MQ=255)

                                                                                                                                           tGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTa      <  1:2266872/149‑1 (MQ=255)

                                                                                                                                              tGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCaa                                    >  2:1660297/1‑116 (MQ=255)

                                                                                                                                              tGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCaa                                    <  1:1660297/116‑1 (MQ=255)

                                                                                                                                              tGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTActct  <  2:2724235/150‑1 (MQ=255)

GCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCAC‑AGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGACTCCTTACTCT  >  NC_002947/5013193‑5013483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`