Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,648,905	(A)6→7	intergenic (‑142/+154)	oprB‑II ← / ← PP_1446	carbohydrate‑selective porin/TonB‑dependent receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,648,899	1	.	A	100.0%	116.0 / NA	36	intergenic (‑136/+160)	oprB‑II/PP_1446	carbohydrate‑selective porin/TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (19/17);  total (19/17)

AGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAG‑AAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTA  >  NC_002947/1648753‑1649035                                                                                                                                                    |                                                                                                                                         aGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAG‑aaa                                                                                                                                       >  2:407855/1‑150 (MQ=255)   ttGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAG‑aaaaa                                                                                                                                     >  2:910897/1‑150 (MQ=255)     ggAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGATGATATAGGGGAAGTTATAAG‑aaaaaaa                                                                                                                                   <  1:848513/150‑2 (MQ=255)     ggAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAG‑aaaaaaa                                                                                                                                   >  1:872929/1‑149 (MQ=255)               aTAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGACGGTTCGATGAAGCGTTTCAGCTTGAGGCGAAGAGCATATGTGTGAAGTTATAAGAAAAAAAGACAAATTGt                                                                                                                          >  1:1422113/1‑150 (MQ=255)                 aGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATGTGGCGACAAGATTGTTCGATGAAGCGTTTCAACTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCg                                                                                                                        <  2:712103/150‑1 (MQ=255)                        tCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGa                                                                                                                 <  2:1300373/150‑1 (MQ=255)                               attattGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGAttgttgt                                                                                                          <  1:451915/150‑1 (MQ=255)                                ttattGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGAttgttgtt                                                                                                         <  1:407855/150‑1 (MQ=255)                                  attGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACACGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAAAGGCCGCAGAGGATTGTTGTTGa                                                                                                       >  1:448408/1‑150 (MQ=255)                                  attGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGa                                                                                                       >  2:655874/1‑150 (MQ=255)                                                 aaGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACCGATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAgtcgt                                                                                        >  1:386147/1‑150 (MQ=255)                                                       aTCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGAGATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTaa                                                                                  >  1:510535/1‑150 (MQ=255)                                                       aTCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGAGGCGGGGACGTTAGAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTaa                                                                                  <  1:910897/150‑1 (MQ=255)                                                          gAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCAtt                                                                                    >  1:219505/1‑145 (MQ=255)                                                          gAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCAtt                                                                                    <  2:219505/145‑1 (MQ=255)                                                                   gcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGACGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGgtg                                                                      >  1:1275207/1‑150 (MQ=255)                                                                    cccatgaaTGGATATGGCTACAAGATTGTTCGATGAAGCGTTTAAGCTTGCGGCAGGGAGGATATAGGGGAACTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGgtgt                                                                     <  2:510535/144‑1 (MQ=255)                                                                       cTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAgg                                                                  >  2:37098/1‑150 (MQ=255)                                                                                  tGGCGACAAGAATGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAAGTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCAc                                                       <  2:505531/150‑1 (MQ=255)                                                                                   ggCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAgg                                                                            <  1:167683/128‑1 (MQ=255)                                                                                   ggCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAgg                                                                            >  2:167683/1‑128 (MQ=255)                                                                                        cAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGa                                                 >  1:81400/1‑150 (MQ=255)                                                                                                   gATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGAttgtt                                                                                                            >  2:320411/1‑80 (MQ=255)                                                                                                   gATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGAttgtt                                                                                                            <  1:320411/80‑1 (MQ=255)                                                                                                         gCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTa                                >  1:878939/1‑150 (MQ=255)                                                                                                          cGTTTAAGCTAGCGGCGGGAAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTAc                               <  2:81400/150‑1 (MQ=255)                                                                                                                aGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCgg                                                             >  2:1376900/1‑114 (MQ=255)                                                                                                                aGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCgg                                                             <  1:1376900/114‑1 (MQ=255)                                                                                                                 gCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTa                        >  1:1401358/1‑150 (MQ=255)                                                                                                                        gcggGGAGGATATAGGGGAAGTTATAAAAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCgg                 <  2:44771/150‑1 (MQ=255)                                                                                                                             gaggaTATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGaa            <  2:448408/150‑1 (MQ=255)                                                                                                                               ggaTATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCggg                                                            <  2:1148792/100‑1 (MQ=255)                                                                                                                               ggaTATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCggg                                                            >  1:1148792/1‑100 (MQ=255)                                                                                                                                  tataGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGt       >  2:328143/1‑150 (MQ=255)                                                                                                                                      ggggAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCt   <  1:190915/150‑1 (MQ=255)                                                                                                                                       gggAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTa  >  2:486080/1‑150 (MQ=255)                                                                                                                                       aggAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTa  >  2:1003102/2‑150 (MQ=255)                                                                                                                                             ttATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATc                           <  2:1241070/119‑1 (MQ=255)                                                                                                                                             ttATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATc                           >  1:1241070/1‑119 (MQ=255)                                                                                                                                                    |                                                                                                                                         AGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAG‑AAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTA  >  NC_002947/1648753‑1649035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑