Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,796,6490AG44.1% 14.2 / 38.3 34intergenic (‑162/+56)pvdH/PP_4224diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (9/10);  new base G (6/9);  total (15/19)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.68e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCGGGCAAGGCTGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGA  >  NC_002947/4796503‑4796796
                                                                                                                                                  |                                                                                                                                                   
ccGGGCAAGGCTGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAAc                                                                                                                                                  >  1:1308351/1‑150 (MQ=255)
      aaGGCTGGAAGCGGTAGACATGGGTAAATTCTCTCGAGCAAGCGGATGGGCGGTTATCGCCTTGGAAACGCTTGAGTGGGGTGCGGATTGAGTGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAAcccgccc                                                                                                                                            <  2:456557/150‑1 (MQ=255)
            ggAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGa                                                                                                                                      <  2:468341/150‑1 (MQ=255)
              aaGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGc                                                                                                                                    >  2:304490/1‑150 (MQ=255)
                 cGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCggg                                                                                                                                 <  1:55049/150‑1 (MQ=255)
                 cGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCggg                                                                                                                                 <  1:304490/150‑1 (MQ=255)
                   gTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGtt                                                                                                                               <  1:223411/150‑1 (MQ=255)
                       aCATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAcc                                                                                                                           <  2:1308351/150‑1 (MQ=37)
                       aCATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAcc                                                                                                                           <  1:899494/150‑1 (MQ=37)
                        cATGGGTAAATCCTCGCGAGCAAGCTGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc                                                                                                                          <  1:1032887/150‑1 (MQ=37)
                             gTAAATCCTCGCGAGCAAGCGGATGGGCGGTTTTCTTTTTGGAAAATCTTGAGTGGGGGGAGAACTAAGCGTTTGTTACGCGATTTTCTTTTTGTCTCTCCTGGCCTATTCCCAGGTAAGCCCGCACTTAGTAGCCGGTTCAGCCACGaa                                                                                                                     <  2:678061/150‑1 (MQ=255)
                             gTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGaa                                                                                                                     <  1:599583/150‑1 (MQ=37)
                                                                            cTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc                                                                                                                          >  2:814716/1‑98 (MQ=18)
                                                                            cTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc                                                                                                                          <  1:814716/98‑1 (MQ=18)
                                                                                                    gttgttACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAAcccgcccg                                                                                                                                           <  1:223041/57‑1 (MQ=255)
                                                                                                    gttgttACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAAcccgcccg                                                                                                                                           >  2:223041/1‑57 (MQ=255)
                                                                                                            gcgATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAagag                                                                                                                  >  1:66687/1‑74 (MQ=255)
                                                                                                            gcgATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAagag                                                                                                                  <  2:66687/74‑1 (MQ=255)
                                                                                                                                         ttCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACgcgc                                                                            <  1:1318789/83‑1 (MQ=14)
                                                                                                                                         ttCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACgcgc                                                                            >  2:1318789/1‑83 (MQ=11)
                                                                                                                                         ttCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGc         >  1:1287702/1‑150 (MQ=37)
                                                                                                                                         ttCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGGGATAAACATCAATGCCGGGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGc         >  1:911224/1‑150 (MQ=255)
                                                                                                                                         ttCGCGGGTAAACCCGCCCGTAGCAGCGGGTTCACCCGCGAAGATGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGc         >  2:130878/1‑150 (MQ=255)
                                                                                                                                            gcgGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCCAGCGGACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGc      >  1:254446/1‑150 (MQ=39)
                                                                                                                                              gggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                               <  1:458172/123‑1 (MQ=25)
                                                                                                                                              gggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                               >  2:458172/1‑123 (MQ=25)
                                                                                                                                              gggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCCCACCGCTGCAc    >  1:627024/1‑150 (MQ=37)
                                                                                                                                              gggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc    <  2:1287702/150‑1 (MQ=37)
                                                                                                                                               ggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                               <  1:354801/122‑1 (MQ=25)
                                                                                                                                               ggTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCg          >  2:100360/1‑143 (MQ=255)
                                                                                                                                               ggTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCg          <  1:100360/143‑1 (MQ=255)
                                                                                                                                                gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACaaaa                                                           <  1:906206/93‑1 (MQ=255)
                                                                                                                                                gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACaaaa                                                           >  2:906206/1‑93 (MQ=255)
                                                                                                                                                gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGa  >  1:1065787/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCGGGCAAGGCTGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGA  >  NC_002947/4796503‑4796796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑