breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | Pputida_HGL_33_S347_L002_R1_001.good.fq | 2,338,139 | 317,201,502 | 100.0% | 135.7 bases | 150 bases | 95.6% |
errors | Pputida_HGL_33_S347_L002_R2_001.good.fq | 2,338,139 | 317,201,502 | 100.0% | 135.7 bases | 150 bases | 93.5% |
total | 4,676,278 | 634,403,004 | 100.0% | 135.7 bases | 150 bases | 94.6% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | NC_002947 | 6,181,929 | 96.9 | 3.8 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
total | 6,181,929 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 60605 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 1147 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.058 |
reference sequence | pr(no read start) |
---|---|
NC_002947 | 0.72770 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
option | value |
---|---|
Mode | Consensus/Mixed Base |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 10 |
Polymorphism frequency cutoff | 0.2 |
Polymorphism minimum variant coverage each strand | OFF |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | OFF |
Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
program | version |
---|---|
bowtie2 | 2.3.4.1 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 20:33:47 20 Aug 2019 | 20:34:50 20 Aug 2019 | 1 minute 3 seconds |
Read alignment to reference genome | 20:34:51 20 Aug 2019 | 20:44:59 20 Aug 2019 | 10 minutes 8 seconds |
Preprocessing alignments for candidate junction identification | 20:44:59 20 Aug 2019 | 20:46:05 20 Aug 2019 | 1 minute 6 seconds |
Preliminary analysis of coverage distribution | 20:46:05 20 Aug 2019 | 20:48:23 20 Aug 2019 | 2 minutes 18 seconds |
Identifying junction candidates | 20:48:23 20 Aug 2019 | 21:06:58 20 Aug 2019 | 18 minutes 35 seconds |
Re-alignment to junction candidates | 21:06:58 20 Aug 2019 | 21:09:41 20 Aug 2019 | 2 minutes 43 seconds |
Resolving best read alignments | 21:09:41 20 Aug 2019 | 21:11:25 20 Aug 2019 | 1 minute 44 seconds |
Creating BAM files | 21:11:25 20 Aug 2019 | 21:13:33 20 Aug 2019 | 2 minutes 8 seconds |
Tabulating error counts | 21:13:33 20 Aug 2019 | 21:14:19 20 Aug 2019 | 46 seconds |
Re-calibrating base error rates | 21:14:19 20 Aug 2019 | 21:14:21 20 Aug 2019 | 2 seconds |
Examining read alignment evidence | 21:14:21 20 Aug 2019 | 21:23:31 20 Aug 2019 | 9 minutes 10 seconds |
Polymorphism statistics | 21:23:31 20 Aug 2019 | 21:23:32 20 Aug 2019 | 1 second |
Output | 21:23:32 20 Aug 2019 | 21:24:11 20 Aug 2019 | 39 seconds |
Total | 50 minutes 23 seconds |