Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 564,385 Δ1 bp 14.9% intergenic (+34/‑139) rplQ → / → katA 50S ribosomal protein L17/catalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947564,3850T.14.9% 134.0 / 19.6 47intergenic (+34/‑139)rplQ/katA50S ribosomal protein L17/catalase
Reads supporting (aligned to +/- strand):  ref base T (16/24);  new base . (3/4);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

GCTACCTGCGCATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGCCCATGA  >  NC_002947/564242‑564527
                                                                                                                                                |                                                                                                                                              
gcTACCTGCGCATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCgg                                                                                                                                          >  1:408616/1‑150 (MQ=255)
      tGCGCATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGtttttt                                                                                                                                    <  1:496969/150‑1 (MQ=255)
      tGCGCATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAACCCGGGCACTAG‑GCCCGGttttct                                                                                                                                    <  1:1457360/150‑3 (MQ=255)
       gcgcATCCTGAAGTGCGGTTTCCGCTCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTg                                                                                                                                   >  2:1380854/1‑150 (MQ=255)
               tGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACa                                                                                                                           >  1:65640/1‑150 (MQ=255)
                gAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACAt                                                                                                                          <  2:65640/150‑1 (MQ=255)
                           tCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTAt                                                                                                               <  1:1110116/150‑1 (MQ=255)
                              gcgcTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGccc                                                                                                                                            >  1:452113/1‑118 (MQ=255)
                              gcgcTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGccc                                                                                                                                            <  2:452113/118‑1 (MQ=255)
                                   ggCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCTGTTTTTTGTTTATACATCTATTGTTTATTTCTATTg                                                                                                       <  2:491399/150‑1 (MQ=255)
                                    gCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTa                                                                                                                <  2:333224/140‑1 (MQ=255)
                                    gCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTa                                                                                                                >  1:333224/1‑140 (MQ=255)
                                    gCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGa                                                                                                      <  1:620204/150‑1 (MQ=255)
                                      gACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATg                                                                                                    <  1:885373/150‑1 (MQ=255)
                                         aaCGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATTTaa                                                                                                 <  2:405972/150‑1 (MQ=255)
                                         aaCGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATCGTTTATTTCGATTGATGTaa                                                                                                 <  2:920102/150‑1 (MQ=255)
                                                             cGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTcc                                                                             <  1:1047062/150‑1 (MQ=255)
                                                             cGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTcc                                                                             <  1:1193210/150‑1 (MQ=255)
                                                             cGAGCTGGTTGATCGTCCGGTCGGCGGTGCAGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTcc                                                                             <  1:1209449/150‑1 (MQ=255)
                                                               aGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCt                                                                           <  2:685856/150‑1 (MQ=255)
                                                                  tGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTa                                                                        >  2:1207657/1‑150 (MQ=255)
                                                                       gATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCtt                                                                   <  1:1207657/150‑1 (MQ=255)
                                                                         tCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGc                                                                 >  1:941797/1‑150 (MQ=255)
                                                                                        tGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATActctct                                                  <  2:1315148/150‑1 (MQ=255)
                                                                                          cTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATActctctct                                                >  1:896797/1‑150 (MQ=255)
                                                                                             tAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACa                                             >  2:539123/1‑150 (MQ=255)
                                                                                             tAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGTTTTCTGta                                                                                                                                 >  2:838744/1‑65 (MQ=17)
                                                                                             tAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGTTTTCTGta                                                                                                                                 >  2:838473/1‑65 (MQ=17)
                                                                                             tAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGTTTTCTGta                                                                                                                                 <  1:838744/66‑2 (MQ=17)
                                                                                             tAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGTTTTCTGta                                                                                                                                 <  1:838473/66‑2 (MQ=17)
                                                                                                  gctgctGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGa                                        <  2:896797/150‑1 (MQ=255)
                                                                                                           tAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTaa                                                                       <  2:1015665/110‑1 (MQ=255)
                                                                                                           tAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTaa                                                                       >  1:1015665/1‑110 (MQ=255)
                                                                                                           tAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGc                               <  1:539123/150‑1 (MQ=255)
                                                                                                                  ttCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGaa                        >  1:163155/1‑150 (MQ=255)
                                                                                                                       tctgCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCg                   >  1:971508/1‑150 (MQ=255)
                                                                                                                           cTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATActctct                                                  >  1:1182092/1‑115 (MQ=255)
                                                                                                                           cTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGCTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATActctct                                                  <  2:1182092/115‑1 (MQ=255)
                                                                                                                            aacaaaaaaCCGGGCACTAG‑GCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGaa                        <  1:1094786/135‑1 (MQ=255)
                                                                                                                            aaCAAAAAACCGGGCACTAG‑GCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGaa                        >  2:1094786/2‑140 (MQ=255)
                                                                                                                                gAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCCCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGgaga          <  2:941797/150‑1 (MQ=255)
                                                                                                                                 aaaaCCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAAt               >  1:574004/1‑144 (MQ=255)
                                                                                                                                 aaaaCCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAAt               <  2:574004/144‑1 (MQ=255)
                                                                                                                                   aaCCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGagag         <  2:945171/148‑1 (MQ=255)
                                                                                                                                   aaCCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGagag         >  1:945171/1‑148 (MQ=255)
                                                                                                                                   aaCCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGcc       >  1:1633294/1‑150 (MQ=255)
                                                                                                                                        ggC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGCCCATGa  <  1:914371/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GCTACCTGCGCATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTATTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGCCCATGA  >  NC_002947/564242‑564527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑