Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	713,108:1	+A	6.7%	coding (94/531 nt)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	713,108	1	.	A	6.7%	245.3 / 9.8	60	coding (94/531 nt)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base . (25/31);  new base A (2/2);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCGGAGCACCCGGAAATCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCAGCTTCCTGGCTGACGGAGCGGGTTGGCAGCGCTGGTTGTTCGCC  >  NC_002947/712961‑713257                                                                                                                                                      |                                                                                                                                                      aTCCGGAGCACCCGGAAATCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑cc                                                                                                                                                     >  1:860356/1‑150 (MQ=255)  tCCGGAGCACCCGGAAATCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑cca                                                                                                                                                    >  1:1136853/1‑150 (MQ=255)  tCCGGAGCACCCGGAAATCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑cca                                                                                                                                                    <  1:1467705/150‑1 (MQ=255)  tCCGGAGCACCCGGAAATCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑cca                                                                                                                                                    >  2:395243/1‑150 (MQ=255)  tCCGGAGCACCCGGAAATCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑cca                                                                                                                                                    >  1:201662/1‑150 (MQ=255)      gAGCACCCGGAAATCTGCTGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGCTCCTGGTC‑ATCGACCAGG‑CCACCaa                                                                                                                                                <  1:122837/150‑1 (MQ=255)         cACCCGGAAATCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCt                                                                                                                                             <  2:1348903/150‑1 (MQ=255)               gAAATCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACtt                                                                                                                                       <  1:1059853/150‑1 (MQ=255)                aaaTCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTc                                                                                                                                      <  2:201662/150‑1 (MQ=255)                  aTCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTcaa                                                                                                                                    <  2:573202/150‑1 (MQ=255)                    cTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTcaaca                                                                                                                                  >  1:1522970/1‑150 (MQ=255)                     tGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGGTGG‑CCTGGTC‑GATGACCAGGACCAGCAAGCtca                                                                                                                                           >  1:800519/1‑138 (MQ=37)                     tGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGGTGG‑CCTGGTC‑GATGACCAGGACCAGCAAGCtca                                                                                                                                           <  2:800519/140‑3 (MQ=37)                         gCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGcc                                                                                                                             <  2:860356/150‑1 (MQ=255)                                  tCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATg                                                                                                                    <  2:37906/150‑1 (MQ=255)                                      cAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCaa                                                                                                                                                <  2:1498061/118‑1 (MQ=255)                                      cAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCaa                                                                                                                                                >  1:1498061/1‑118 (MQ=255)                                              ccGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCaa                                                                                                                                                <  2:1492350/110‑1 (MQ=255)                                              ccGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCaa                                                                                                                                                >  1:1492350/1‑110 (MQ=255)                                              ccGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACcagca                                                                                                            >  2:776698/1‑146 (MQ=255)                                              ccGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACcagca                                                                                                            <  1:776698/146‑1 (MQ=255)                                              ccGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATc                                                                                                        >  1:548539/1‑150 (MQ=255)                                              ccGGCGCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATc                                                                                                        <  2:360644/150‑1 (MQ=255)                                                    cGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTc                                                                                                  <  2:297504/150‑1 (MQ=255)                                                        tGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGTACGACTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTc                                                                                              >  1:380212/1‑150 (MQ=255)                                                            gTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGa                                                                                          <  1:632946/150‑1 (MQ=255)                                                            gTGCGCAACTAAGCCCTACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGa                                                                                          <  2:1275322/150‑1 (MQ=255)                                                             tGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCGTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGac                                                                                         <  2:157577/150‑1 (MQ=255)                                                                gcCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGAc                                                                                                                        >  1:423007/1‑116 (MQ=255)                                                                gcCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGAc                                                                                                                        <  2:423007/116‑1 (MQ=255)                                                                gcCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGactac                                                                                      >  2:971300/1‑150 (MQ=255)                                                                 ccACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGGTGG‑CCTGGTCGAT‑GACCAGGACCAGCAAGCtca                                                                                                                                           >  1:1473781/1‑94 (MQ=18)                                                                 ccACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGGTGG‑CCTGGTCGAT‑GACCAGGACCAGCAAGCtca                                                                                                                                           <  2:1473781/96‑3 (MQ=18)                                                                      aTGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGc                                                                                <  2:1522970/150‑1 (MQ=255)                                                                       tGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCt                                                                               >  2:1580893/1‑150 (MQ=255)                                                                       tGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCt                                                                               <  1:180591/150‑1 (MQ=255)                                                                         ccTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTgg                                                                             >  2:231624/1‑150 (MQ=255)                                                                          cTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCGTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGa                                                                            >  2:1098277/1‑150 (MQ=255)                                                                          cTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGa                                                                            >  2:1552767/1‑150 (MQ=255)                                                                               ccTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTg                                                                       <  1:1098277/150‑1 (MQ=255)                                                                                cTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTgg                                                                      >  2:29507/1‑150 (MQ=255)                                                                                     gCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTac                                                                 <  2:18931/150‑1 (MQ=255)                                                                                     gCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTAAATACCCAAAATGCTTACTATTTACGAGCATTTCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTac                                                                 >  1:603611/1‑150 (MQ=255)                                                                                          gcgcTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAacac                                                            >  1:230736/1‑150 (MQ=255)                                                                                              ttCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGt                                                        >  2:965183/1‑150 (MQ=255)                                                                                              ttCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGt                                                        >  1:383305/1‑150 (MQ=255)                                                                                                      ccTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGtt                                                <  2:1136853/150‑1 (MQ=255)                                                                                                       cTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGa                                                                                          >  1:854778/1‑107 (MQ=255)                                                                                                       cTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGa                                                                                          <  2:854778/107‑1 (MQ=255)                                                                                                        ttGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCa                                              <  2:230736/150‑1 (MQ=255)                                                                                                        ttGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCACGTTCa                                              <  1:231624/150‑1 (MQ=255)                                                                                                            aTGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTc                                                                                              <  1:425586/98‑1 (MQ=255)                                                                                                            aTGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTc                                                                                              >  2:425586/1‑98 (MQ=255)                                                                                                                     gctgAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCAGCTTCCTGGCTGa                                 <  1:354379/150‑1 (MQ=255)                                                                                                                     gctgAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCAGCTTCCTGGCTGa                                 >  1:809991/1‑150 (MQ=255)                                                                                                                           cTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCAGCTTCCTGGCTGACGGAGc                           <  1:395243/150‑1 (MQ=255)                                                                                                                             tGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCAGCTTCCTGGCTGACGGAGCgg                         <  1:965183/150‑1 (MQ=255)                                                                                                                              gCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTc                                                                                   <  2:845427/91‑1 (MQ=255)                                                                                                                              gCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTc                                                                                   >  1:845427/1‑91 (MQ=255)                                                                                                                                  gtcctggtc‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCt                                                                               <  1:464676/91‑1 (MQ=255)                                                                                                                                  gtcctggtc‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCt                                                                               >  2:464676/1‑91 (MQ=255)                                                                                                                                         tc‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGc                                                                     <  1:73519/94‑1 (MQ=255)                                                                                                                                         tc‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGc                                                                     >  2:73519/1‑94 (MQ=255)                                                                                                                                               gACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCAGCTTCCTGGCTGACGGAGCGGGTTGGCAGCGCTGgttg        >  1:792656/1‑150 (MQ=255)                                                                                                                                                aCCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCAGCTTCCTGGCTGACGGAGCGGGTTGGCAGCGCTGgttgt       >  1:1181032/1‑150 (MQ=255)                                                                                                                                                    gg‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCAGCTTCCTGGCTGACGGAGCGGGTTGGCAGCGCTGGTTGTTCGc   <  2:1181032/150‑1 (MQ=255)                                                                                                                                                     g‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCAGCCGGCGGGCTGACGGAGCGGGTTGGCAGCGCTGGTAGATCgga  >  1:1251377/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                                      ATCCGGAGCACCCGGAAATCTGCGGCCGTTGCGTCGACAACCTGACCGGCTCGGGTGAGGTGCGCCACTATGCCTAACCCTGCAGCGGGGCGCTTCGGGCGCCTTGCATGGCTGTGGCTGAGCTTGCTGGTCCTGGTC‑ATCGACCAGG‑CCACCAAGCTGTACTTCAACAACGCCCTGACCATGTACCAGCAGATCGTGGTCATTCCTGACTACTTCAGCTGGACCCTGGCTTACAACACCGGTGCCGCGTTCAGCTTCCTGGCTGACGGAGCGGGTTGGCAGCGCTGGTTGTTCGCC  >  NC_002947/712961‑713257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑