| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 26,837:1 | +A | 8.1% | intergenic (+33/+152) | PP_0021 → / ← PP_0022 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 26,837 | 1 | . | A | 8.1% | 246.1 / 16.0 | 62 | intergenic (+33/+152) | PP_0021/PP_0022 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (29/28); new base A (2/3); total (31/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-01 | |||||||||||
GAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGT > NC_002947/26700‑26985 | gAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTgg < 1:27111/149‑1 (MQ=255)gAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTgg > 2:27111/1‑149 (MQ=255) aTCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGttt > 2:1304000/1‑150 (MQ=255) tCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTa < 2:738832/150‑1 (MQ=255) ccTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACa < 1:1147367/150‑1 (MQ=255) tATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGTAAACCACTGTGG‑TTACAGTg < 2:1263494/150‑1 (MQ=255) aTGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGaaaaaaa < 2:1455534/150‑1 (MQ=255) gCCGGTTGATCATCCTTATCGAAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTACTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAAACACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGt < 2:740138/150‑1 (MQ=255) tGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTa < 1:545735/150‑1 (MQ=255) catcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCCCAGTGGTGTACAGTGAAAAAAAGTGGTTTACAAc > 2:196974/1‑150 (MQ=255) catcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAAc < 1:1139093/150‑1 (MQ=255) tcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTTACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAAc < 2:625955/148‑1 (MQ=255) tcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTAc > 2:495338/1‑145 (MQ=255) tcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTAc < 1:495338/145‑1 (MQ=255) tcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAAc > 1:625955/1‑148 (MQ=255) tcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGt < 1:1304000/150‑1 (MQ=255) ttATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGc > 1:289802/1‑150 (MQ=255) gCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTgg > 1:941447/1‑132 (MQ=255) gCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTgg < 2:941447/132‑1 (MQ=255) aaTGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACGACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACAcg > 1:824137/1‑150 (MQ=255) aTAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTgg < 1:345670/123‑1 (MQ=255) aTAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTgg > 2:345670/1‑123 (MQ=255) tGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCa > 1:1129983/1‑150 (MQ=255) ccGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGc > 2:1317311/1‑150 (MQ=255) ccGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGc > 1:626783/1‑150 (MQ=255) cGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑aa > 1:703762/1‑77 (MQ=255) cGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑aa < 2:703762/77‑1 (MQ=255) gggAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCa < 2:1129983/150‑1 (MQ=255) aCTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGct > 1:380655/1‑150 (MQ=255) cTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGctc < 2:289802/150‑1 (MQ=255) gATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCAtt < 1:354477/150‑1 (MQ=255) gAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTg > 1:1147458/1‑61 (MQ=255) gAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTg < 2:1147458/61‑1 (MQ=255) gACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCg > 1:571097/1‑150 (MQ=255) aCTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCgg < 2:571097/150‑1 (MQ=255) tCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGc > 1:194348/1‑150 (MQ=255) gCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCg > 2:1011080/1‑150 (MQ=255) gCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCg > 1:1510664/1‑150 (MQ=255) cTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTa > 2:156326/1‑83 (MQ=255) cTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTa < 1:156326/83‑1 (MQ=255) gCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGaaaaaaa > 2:1560023/1‑56 (MQ=255) gCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGaaaaaaa < 1:1560023/56‑1 (MQ=255) tacgttgtAAACCACTTTTTTTCACTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCAtt < 1:160287/115‑1 (MQ=18) tacgttgtAAACCACTTTTTTTCACTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCAtt > 2:160287/4‑118 (MQ=18) cTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTAtgct > 1:1032085/1‑150 (MQ=255) gtAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGc > 1:782342/1‑84 (MQ=255) gtAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGc < 2:782342/84‑1 (MQ=255) gtAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGc > 1:782379/1‑84 (MQ=255) gtAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGc < 2:782379/84‑1 (MQ=255) tAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGGCGGGCGTCGAACACCTATGCTGCTCgg > 1:304608/1‑150 (MQ=255) aaCCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGc < 1:92157/93‑1 (MQ=255) aaCCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGc > 2:92157/1‑93 (MQ=255) aCCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGc > 1:1215737/1‑150 (MQ=255) aCCACTTTTTCGCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTAAGACACGCGCGCTGGCCACTCCCCAGTCCAGCGCGCTCCTCATTGATTCCTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGc < 1:196974/150‑1 (MQ=39) cACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGtt < 1:1150451/114‑1 (MQ=255) cACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGtt > 2:1150451/1‑114 (MQ=255) aCTTTTTTTCACTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGc < 2:20738/77‑1 (MQ=14) aCTTTTTTTCACTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGc > 1:20738/1‑77 (MQ=14) tttttttCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGc < 2:1215737/150‑1 (MQ=255) ttttttCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGcc > 1:1204352/1‑150 (MQ=255) aCTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCAcgccgc < 2:626783/150‑1 (MQ=255) tGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAgcgcg > 1:1298035/1‑84 (MQ=255) tGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAgcgcg < 2:1298035/84‑1 (MQ=255) gT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGgt < 1:1011080/150‑1 (MQ=255) | GAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGT > NC_002947/26700‑26985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |