| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,606,122 | G→T | 6.0% | H690N (CAT→AAT) | cyaB ← | cyclolysin secretion/processing ATP‑binding protein CyaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,606,122 | 0 | G | T | 6.0% | 214.7 / 3.2 | 67 | H690N (CAT→AAT) | cyaB | cyclolysin secretion/processing ATP‑binding protein CyaB |
| Reads supporting (aligned to +/- strand): ref base G (30/33); new base T (2/2); total (32/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01 | |||||||||||
GTCCTTGAACGCCACACTCATACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAGGATGCGTGGGTTGCCGAACA > NC_002947/5605977‑5606268 | gTCCTTGAACGCCACACTCATACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATACCCAAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGt > 1:926486/1‑150 (MQ=255) cTTGAACGCCACACTCATACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACt < 1:1219086/150‑1 (MQ=255) ttGAACGCCACACTCATACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTg > 2:30132/1‑150 (MQ=255) aaCGCCACACTCATACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCg > 1:1092409/1‑150 (MQ=255) gCCACACTCATACGGCGGCCCCGAGCTGCAGTTGATGCAGGTCGGTATCAATCCCCTGTGTATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGaaa < 2:42497/150‑1 (MQ=255) gCCACACTCATACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGaaa > 1:399462/1‑150 (MQ=255) acacTCATACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGcc > 2:314599/1‑150 (MQ=255) aTACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATggg > 2:1618379/1‑150 (MQ=255) aTACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATg > 2:1057008/1‑148 (MQ=255) aTACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATg < 1:1057008/148‑1 (MQ=255) ccccGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAatgatga < 1:1618379/150‑1 (MQ=255) cgctaGCCGCAGGTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTAATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAatgatgat < 2:138931/146‑1 (MQ=255) aGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGAcc < 1:606870/150‑1 (MQ=255) ggTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGt > 1:830016/1‑136 (MQ=255) ggTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGt < 2:830016/136‑1 (MQ=255) ggTGCCCTGTGGATACCACCGCCGTGTATCATGAGACCATTGCTTTCTGATCTGCCCGCGCTCCATGACCACTGTGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCCATGACGCTGCCCGTCCGCTCCGCACAAATCCGCGCCAt < 2:919854/150‑1 (MQ=255) gTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATg > 2:1258625/1‑150 (MQ=255) gCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTACCCAAAGACGATTTTCATGAAAGAGGACGGGGCTCTTCGCGCAAATCCGCGCCacgct > 2:1089493/1‑146 (MQ=255) tgtgGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATTGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTctgc > 1:883502/1‑150 (MQ=255) tgtgGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATTGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTctgc > 1:884451/1‑150 (MQ=255) tgtgGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTctgc > 2:1631157/1‑150 (MQ=255) tgtgGATAACCCAGCAGCTCATCATGAGTCACATGATCGGTTGTCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTctgc > 1:723325/1‑150 (MQ=255) tgGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTctgctg > 1:722528/1‑150 (MQ=255) tgGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTctgctg < 2:1092409/150‑1 (MQ=255) gATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGcc < 1:1577108/93‑1 (MQ=255) gATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGcc > 2:1577108/1‑93 (MQ=255) aCCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAAtgatgatga < 2:1447500/107‑1 (MQ=255) aCCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAAtgatgatga > 1:1447500/1‑107 (MQ=255) cccAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATc < 2:1568750/150‑1 (MQ=255) cagcTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTc < 1:314599/150‑1 (MQ=255) tcatcaTGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTctg > 1:1309418/1‑131 (MQ=255) tcatcaTGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTctg < 2:1309418/131‑1 (MQ=255) atcatGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAAtgatgatga < 1:1399810/95‑1 (MQ=255) atcatGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAAtgatgatga > 2:1399810/1‑95 (MQ=255) tcatGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGAGCTCGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCa < 1:1089493/150‑1 (MQ=255) tcatGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCa < 2:399462/150‑1 (MQ=255) atGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGCTCGGTAATGATGATAAACCTACAGTTCGCCCGAATCCGCGCCAGGTTCTGCTGGATGATCCGTTCGGACTCATa < 1:30132/150‑1 (MQ=255) aGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCGCGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGc < 1:1566885/78‑1 (MQ=255) aGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGc > 2:1566885/1‑78 (MQ=255) gTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCAt < 2:321416/116‑1 (MQ=255) gTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCAt > 1:321416/1‑116 (MQ=255) ccTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAgc > 1:1159151/1‑150 (MQ=255) gatgatCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACtggt < 2:926486/150‑1 (MQ=255) gatgatCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACtggt < 1:1258625/150‑1 (MQ=255) tCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCtt < 1:1631157/150‑1 (MQ=255) cccACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTc < 1:1464469/100‑1 (MQ=255) cccACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTc > 2:1464469/1‑100 (MQ=255) gCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGaaaa < 1:1567542/150‑1 (MQ=255) tCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGa < 2:983595/113‑1 (MQ=255) tCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGa > 1:983595/1‑113 (MQ=255) aTGACCACGATGCGGTCGGCATTGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAgg < 2:884451/150‑1 (MQ=255) aTGACCACGATGCGGTCGGCATTGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAgg < 2:883502/150‑1 (MQ=255) aTGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAgg > 1:1464098/1‑150 (MQ=255) cACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAatgatgat < 2:307689/51‑1 (MQ=255) cACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAatgatgat > 1:307689/1‑51 (MQ=255) cACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCATCGCACGTGTGGCTTCGTCGAAAATGAGGaagag > 2:268526/1‑146 (MQ=255) cGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAGGATGCGTg < 1:64638/150‑1 (MQ=255) gctGCGGTCGGTATGTCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCAATGTTCTGCTGGATGATTCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAGGATGCGTgg < 1:368128/148‑1 (MQ=255) gATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCGGCGCGCAAATCCGCGCCATGTTCTGCTGAATGATTCGTTCGGACTCATACTCCAGCGCACTGGCGGCTTCGTCGAAAATGAGGATGCGTgg > 1:537501/1‑150 (MQ=255) aTGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCAt > 1:371218/1‑77 (MQ=255) aTGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCAt < 2:371218/77‑1 (MQ=255) ggTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAGGATGCGTGGGTTGc > 2:1065476/1‑150 (MQ=255) gTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAGGATGCGTGGGTTGcc > 1:281454/1‑150 (MQ=255) ggCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAGGATGCGTGGGTTGCCGaa < 1:1065476/150‑1 (MQ=255) gCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAGGATGCGTGGGttatccaca < 2:723325/150‑8 (MQ=255) cATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAGGATGCGTGGGTTGCCGAACa < 2:722528/150‑1 (MQ=255) cATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTATGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAGGATGCGTGGGTTGCCGAACa > 2:400190/1‑150 (MQ=255) | GTCCTTGAACGCCACACTCATACGGCGGCCCCGAGCTGCAGTTGATGCAGGTAGGCATAGGTGCCCTGTGGATAACCCAGCAGCTCATCATGAGTCCCTTGCTCGATGATCTGCCCACGCTCCATGACCACGATGCGGTCGGCATGGCGTACTGCCGAAAGCCGATGGGCAATGATGATGACCGTGCGCTGCGCGCAAATCCGCGCCATGTTCTGCTGGATGATCCGTTCGGACTCATAGTCCAGCGCACTGGTGGCTTCGTCGAAAATGAGGATGCGTGGGTTGCCGAACA > NC_002947/5605977‑5606268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |