Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,914,167 G→A 10.9% intergenic (+3/+150) spuI → / ← argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,914,1670GA10.9% 142.3 / 7.6 55intergenic (+3/+150)spuI/argAglutamylpolyamine synthetase/amino acid N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (21/28);  new base A (2/4);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

AGCTGGACGACAGCGAGATCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGG  >  NC_002947/5914022‑5914305
                                                                                                                                                 |                                                                                                                                          
aGCAGGCCGACAGCGAGAGCCTGAACAAGTACATCGATCCGAAGTAAATCCACATCTTCGTCGCGTGCAAGGAAAGCAAGCTGGAAGAGTTCAACCACTCTAACTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGaaaa                                                                                                                                        <  1:583435/150‑1 (MQ=255)
           aGCGAGATCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGCTCTACGAACTCCAGTATGCCTGTTCTCAAGGTACCGTGTAACCGGAAACGCCGCCCCTc                                                                                                                             <  2:837659/150‑1 (MQ=255)
           aGCGAGATCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTc                                                                                                                             >  1:217727/1‑150 (MQ=255)
                 aTCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGggc                                                                                                                       <  1:187330/150‑1 (MQ=255)
                  tCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAggg                                                                                                                        <  1:1375194/148‑1 (MQ=255)
                  tCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAggg                                                                                                                        >  2:1375194/1‑148 (MQ=255)
                    cTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGggcggc                                                                                                                    >  2:441802/1‑150 (MQ=255)
                     tGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGgcggcg                                                                                                                   <  2:217727/150‑1 (MQ=255)
                     tGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACAAAAACGCCGCCCTTGAGGGgcggcg                                                                                                                   >  2:605354/1‑150 (MQ=255)
                       aaCAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTTGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTTAAGGGCGGCGtt                                                                                                                 <  1:1327474/150‑1 (MQ=255)
                       aaCAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACAAAAACGCCGCCCTTGAGGGGCGGCGtt                                                                                                                 <  1:605354/150‑1 (MQ=39)
                          aaGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACAAAAACGCCGCCCTTGAGGGGCGGCGttttc                                                                                                              <  2:134473/150‑2 (MQ=38)
                           aGTACTTAGATCCTAAATACATTGCCATTTTCCTCGCGTGCAAGTAAAGCGAGCTGGAATTGTTCTAGCCCTCGATCTCCGACCTCCAGTACAACTGGTACCTGCATACCGTGTAAACAAATACGCCGCCCTTGAGGGGCGGCGttttcg                                                                                                             <  1:540850/150‑3 (MQ=255)
                                aTCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTga                                                                                                        <  1:798831/150‑1 (MQ=255)
                                     tCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCg                                                                                                   <  1:1609344/150‑1 (MQ=255)
                                      ccGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCgg                                                                                                  <  1:660806/150‑1 (MQ=255)
                                               aTCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCct                                                                                         >  1:1522269/1‑150 (MQ=255)
                                                           gtcgCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCAc                                                                             <  1:441802/150‑1 (MQ=255)
                                                              gcgTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGGCCCTCAAggg                                                                                                                        <  1:164327/104‑1 (MQ=255)
                                                              gcgTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAggg                                                                                                                        >  2:164327/1‑104 (MQ=255)
                                                                 tGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGc                                                                       >  2:802130/1‑150 (MQ=255)
                                                                    aaGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTcc                                                                    >  1:8949/1‑150 (MQ=255)
                                                                          aGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAgg                                                              <  2:1522269/150‑1 (MQ=255)
                                                                          aGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAgg                                                              <  2:701777/150‑1 (MQ=255)
                                                                            cGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGt                                                            <  1:802130/150‑1 (MQ=255)
                                                                                        gTTCGAGCACTCGATCTCCGACCTCGATTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTga                                                                                                        <  2:666422/94‑1 (MQ=255)
                                                                                        gTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTga                                                                                                        >  1:666422/1‑94 (MQ=255)
                                                                                            gAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCCGCCGCCGGGCACTTTCGCAggatcga                                            >  2:300201/1‑145 (MQ=255)
                                                                                                  tCGATCTCCCACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                      <  1:213773/150‑1 (MQ=255)
                                                                                                    gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTcgc                                                                                   >  2:573043/1‑103 (MQ=255)
                                                                                                    gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTcgc                                                                                   <  1:573043/103‑1 (MQ=255)
                                                                                                    gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGa                                    >  2:1301884/1‑150 (MQ=255)
                                                                                                    gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGa                                    <  1:300201/150‑1 (MQ=255)
                                                                                                       ctcCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACtt                                 >  1:13007/1‑150 (MQ=255)
                                                                                                        tcCGACGGCGAGTACGACTGGTACCTGGATAGCGGGTAAAGGAAAACGCCGCCCCTCAAGGGCGGCGTTGTTGTTTGAGACCGGTGTGGCCCTCTTGGCGGGCAGGCCCGCTGCCGCAGGGGAATTTGGAGGGCTGAAGCGTGTGACTTa                                >  2:1256453/1‑150 (MQ=255)
                                                                                                        tcCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTa                                <  1:1301884/150‑1 (MQ=255)
                                                                                                         ccGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTAc                               <  2:13007/150‑1 (MQ=255)
                                                                                                             ccTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                      <  1:1119899/139‑1 (MQ=255)
                                                                                                             ccTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCACGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                      >  2:1119899/1‑139 (MQ=255)
                                                                                                                    tACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                      <  1:461498/132‑1 (MQ=255)
                                                                                                                    tACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                      >  2:461498/1‑132 (MQ=255)
                                                                                                                          tGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTg              >  1:1077977/1‑150 (MQ=255)
                                                                                                                                cTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCcgcg        <  2:1077977/150‑1 (MQ=255)
                                                                                                                                     tACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCggg                  >  1:262793/1‑135 (MQ=255)
                                                                                                                                     tACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCggg                  <  2:262793/135‑1 (MQ=255)
                                                                                                                                         tctcaaaCAAAAACGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgtg                          <  1:640915/119‑1 (MQ=37)
                                                                                                                                         tctcaaaCAAAAACGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgtg                          >  2:640915/5‑123 (MQ=37)
                                                                                                                                          tgtAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                      >  1:1503967/1‑110 (MQ=255)
                                                                                                                                          tgtAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt                                      <  2:1503967/110‑1 (MQ=255)
                                                                                                                                          tgtAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgt                           <  2:367081/121‑1 (MQ=255)
                                                                                                                                          tgtAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgt                           >  1:367081/1‑121 (MQ=255)
                                                                                                                                              aaCGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGt               <  1:1550189/129‑1 (MQ=255)
                                                                                                                                              aaCGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGt               >  2:1550189/1‑129 (MQ=255)
                                                                                                                                                cGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAgg  <  2:1557815/140‑1 (MQ=255)
                                                                                                                                                cGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCGCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAgg  >  1:1557815/1‑140 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
AGCTGGACGACAGCGAGATCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGG  >  NC_002947/5914022‑5914305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑