Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,984,759 A→C 7.3% D139E (GAT→GAG)  ycaC‑II ← putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,984,7590AC 7.3% 191.3 / 5.8 68D139E (GAT→GAG) ycaC‑IIputative hydrolase
Reads supporting (aligned to +/- strand):  ref base A (27/36);  new base C (2/3);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

CAGGTTACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAG  >  NC_002947/5984615‑5984903
                                                                                                                                                |                                                                                                                                                
cagGTTACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCTTTGAAGGTGCCCGAGGCATCGGt                                                                                                                                             <  1:1318599/150‑1 (MQ=255)
 agGTTACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTc                                                                                                                                            <  2:551250/150‑1 (MQ=255)
   gTTACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTcac                                                                                                                                          >  1:454885/1‑150 (MQ=255)
     tACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTcacca                                                                                                                                        <  2:475687/150‑1 (MQ=255)
     tACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTcacca                                                                                                                                        <  1:1498011/150‑1 (MQ=255)
       cccAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACg                                                                                                                                      >  1:1336958/1‑150 (MQ=255)
         cAGCCCTTCGATGTCGGTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGACCGATGCCTCGGGcaccttcaa                                                                                                                                    <  2:698086/150‑6 (MQ=38)
           gCCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAaca                                                                                                                                  <  2:1336958/150‑1 (MQ=255)
              cTTCGATGTCGTTGCGCCGGTCGCGGTGCAGTTCGCAGGCCACCGAGAGCCTGTTCATCATCTGCGCCCCCGCCTGGGTCATCCGTACCCAAGCGGGTTGCTGCACCGTTTCGTTGAAGGTGTCGGAGGCAGCGGTCACCTCGacaacta                                                                                                                               >  2:151807/1‑144 (MQ=25)
               ttCGATGTCGTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTc                                                                                                                              >  1:607791/1‑150 (MQ=255)
                        gTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGCACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACAGTTTCGTTGAAGGTGCCCCAGGCATCGGAAACCACGAACACTTCAAAACCTTc                                                                                                                     >  2:378374/1‑150 (MQ=39)
                        gTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTc                                                                                                                     <  2:607791/150‑1 (MQ=255)
                         ttGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTcc                                                                                                                    <  2:164881/150‑1 (MQ=255)
                         ttGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTcc                                                                                                                    >  1:164881/1‑150 (MQ=255)
                         ttGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTcc                                                                                                                    <  1:1315291/150‑1 (MQ=255)
                                 gTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAgcgc                                                                                                            <  1:27315/150‑1 (MQ=255)
                                                  cggCCAAGGAGAACGATTGGATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAgg                                                                                           <  2:41508/149‑1 (MQ=255)
                                                   ggCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACTAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGc                                                                                          >  1:917482/1‑150 (MQ=255)
                                                    gCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTc                                                                                                                     >  2:22679/1‑122 (MQ=255)
                                                    gCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTc                                                                                                                     <  1:22679/122‑1 (MQ=255)
                                                     ccACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCAAGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCa                                                                                        <  1:378374/150‑1 (MQ=255)
                                                     ccACCGAGAACCAGTTCATCATCTGCGCACACGCCTGGGTTGTGCGTACCGAACCCGCTCGCTGCACGGTTTCGTGGAAGGTGCCCGAGGAATCGGTCACCACGAACACTTCCACCCCTTACGCTACCGCCGACAGCGTCGGGAAGGCCa                                                                                        <  2:1177337/150‑1 (MQ=255)
                                                      cACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCgg                                                                                                <  2:1066282/141‑1 (MQ=255)
                                                      cACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCgg                                                                                                >  1:1066282/1‑141 (MQ=255)
                                                       aCCGAGAACCAGTTCATCATCTGCGCACGCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGt                                                                                                                                             >  2:1296928/1‑95 (MQ=255)
                                                       aCCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGt                                                                                                                                             <  1:1296928/95‑1 (MQ=255)
                                                                cAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCaaaa                                                                                                                          >  2:654023/1‑105 (MQ=255)
                                                                cAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCaaaa                                                                                                                          <  1:654023/105‑1 (MQ=255)
                                                                 aGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGc                                                                                          >  2:1508060/1‑136 (MQ=255)
                                                                 aGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGc                                                                                          <  1:1508060/136‑1 (MQ=255)
                                                                    tcatcaTCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTca                                                                         <  1:148078/150‑1 (MQ=255)
                                                                     catcatCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTcac                                                                        >  2:1099509/1‑150 (MQ=255)
                                                                        catcTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAgg                                                                                           >  2:677967/1‑128 (MQ=255)
                                                                        catcTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAgg                                                                                           <  1:677967/128‑1 (MQ=255)
                                                                         atcTGCGCACCCGCCTGGGTCATGCGTACCCCAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACcaca                                                                    <  2:1253339/150‑1 (MQ=255)
                                                                         atcTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCAACGATTTGGTGAAGGTGGCCTATTTAGCTGTCACCACGAACACTTCAAAACCTTTCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACcaca                                                                    >  1:444261/1‑150 (MQ=255)
                                                                          tcTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCacac                                                                   >  1:901994/1‑150 (MQ=255)
                                                                          tcTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCacac                                                                   <  2:444261/150‑1 (MQ=255)
                                                                                cACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGACCGATGCCTCGGGcac                                                                                                                                          >  2:846569/1‑73 (MQ=38)
                                                                                cACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGACCGATGCCTCGGGcac                                                                                                                                          <  1:846569/73‑1 (MQ=38)
                                                                                 aCCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCgat                                                            >  1:127640/1‑150 (MQ=255)
                                                                                 aCCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCg                                                              >  1:441421/1‑148 (MQ=255)
                                                                                 aCCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCg                                                              <  2:441421/148‑1 (MQ=255)
                                                                                   ccGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGGTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCgatga                                                          <  2:659240/150‑1 (MQ=255)
                                                                                                 cGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCaaaa                                                                                                                          <  2:988659/72‑1 (MQ=255)
                                                                                                 cGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCaaaa                                                                                                                          >  1:988659/1‑72 (MQ=255)
                                                                                                      ccAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCgat                                                            <  2:186841/129‑1 (MQ=255)
                                                                                                      ccAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCgat                                                            >  1:186841/1‑129 (MQ=255)
                                                                                                       cAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGACCGATGCCTCGGGcacc                                                                                                                                         >  1:1100235/1‑51 (MQ=18)
                                                                                                       cAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGACCGATGCCTCGGGcacc                                                                                                                                         <  2:1100235/51‑1 (MQ=18)
                                                                                                        aaGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCAc                                                                                       <  1:719667/100‑1 (MQ=255)
                                                                                                        aaGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCAc                                                                                       >  2:719667/1‑100 (MQ=255)
                                                                                                         aGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGt                                                                                                   >  2:41219/1‑87 (MQ=255)
                                                                                                         aGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGt                                                                                                   <  1:41219/87‑1 (MQ=255)
                                                                                                            cGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCCCCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCtt                                 <  2:917482/150‑1 (MQ=255)
                                                                                                                    gcACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCt                         <  1:1099509/150‑1 (MQ=255)
                                                                                                                    gcACGGTTGCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCt                         <  1:151807/150‑1 (MQ=255)
                                                                                                                       cGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGa                      <  2:619021/150‑1 (MQ=255)
                                                                                                                           ttCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGaa                  <  2:372057/150‑1 (MQ=255)
                                                                                                                              gTTGAAGGTGCCCGAGGCATCGGTCAACACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTc               >  1:457959/1‑150 (MQ=255)
                                                                                                                               ttGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGc                                                                                     >  2:570126/1‑79 (MQ=255)
                                                                                                                               ttGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGc                                                                                     <  1:570126/79‑1 (MQ=255)
                                                                                                                               ttGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCt              <  2:901994/150‑1 (MQ=255)
                                                                                                                               ttGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCt              >  1:560896/1‑150 (MQ=255)
                                                                                                                                tGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCtt             <  2:560896/150‑1 (MQ=255)
                                                                                                                                 gAACGTGCCCGAGGCATCGGTCACCACGAAAACTGCAAAACCTTCCGCCAGCGCAGACATGGTCGGGAAGGCCACGCACACATCGTTCACCACACCGGCGATGGTCAGTTGCTTGCGGCCCTTGGCCTTGATTGCCTTGACGAAGTCTTc            >  2:81130/1‑150 (MQ=37)
                                                                                                                                           gAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGCCGTGGTAGTGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAg  >  1:837464/1‑150 (MQ=37)
                                                                                                                                           gAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGCCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAg  <  1:1049554/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CAGGTTACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGTTCGCAGGCCACCGAGAACCAGTTCATCATCTGCGCACCCGCCTGGGTCATGCGTACCCAAGCCGCTTGCTGCACGGTTTCGTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAG  >  NC_002947/5984615‑5984903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑