| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,065,595 | A→C | 10.0% | E147A (GAG→GCG) ‡ | phoB → | two‑component system DNA‑binding response regulator PhoB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,065,595 | 0 | A | C | 10.0% | 158.0 / 11.6 | 60 | E147A (GAG→GCG) ‡ | phoB | two‑component system DNA‑binding response regulator PhoB |
| Reads supporting (aligned to +/- strand): ref base A (28/26); new base C (3/3); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01 | |||||||||||
CGCCAAGGGTGAAGAGGACAACAAGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCCTGCTGCAGTTTTTCATGACCCACCAGGAGCGCGCCTATACCCGCGGCCAACTGCTGGATCAG > NC_002947/6065446‑6065743 | cGCCAAGGGTGAAGAGGACAACAAGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGAGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGa < 1:385914/150‑1 (MQ=255) ccAAGGGTGAAGAGGACAACAGGATCCAGGGCCTGGAGGTTGGCGCCGACGACGACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAgg > 1:1270919/1‑150 (MQ=255) gggTGAAGAGGACAACAAGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGtcgg < 1:131277‑M2/150‑5 (MQ=255) gTGAAGAGGACAACAAGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGtcggcg > 2:1270919‑M2/1‑144 (MQ=255) agGACAACAAGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCctgc > 2:1602634/1‑150 (MQ=255) gACAACAAGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGcc > 1:483937/1‑145 (MQ=255) gACAACAAGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGcc < 2:483937/145‑1 (MQ=255) gACAACAAGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCctgctg > 1:234364/1‑150 (MQ=255) aacaaGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGATCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTc > 1:705886/1‑150 (MQ=255) caaGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGa > 1:145665/1‑150 (MQ=255) caaGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGa > 1:1244941/1‑150 (MQ=255) aaGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGAc > 1:274000/1‑150 (MQ=255) aaGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGACCTCGAtcggcgcctcg > 2:1607772‑M2/1‑130 (MQ=255) aaGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGACCTCGAtcggcgcctcg < 1:1607772‑M2/141‑12 (MQ=255) gATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGAccc > 2:1588020/1‑150 (MQ=255) aGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGTCAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATcg > 1:872939/1‑149 (MQ=255) aGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCa > 1:473739/1‑150 (MQ=255) ccTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGTCAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATcggcgc < 2:872939/150‑6 (MQ=255) ccTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCa < 2:701105/150‑1 (MQ=255) cTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCAc > 1:1049471/1‑150 (MQ=255) ggAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCg > 2:927442/1‑150 (MQ=255) tGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGa < 2:282492/150‑1 (MQ=255) tGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGa < 1:1249165/150‑1 (MQ=255) gggCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGAc > 2:1086025/1‑150 (MQ=255) gacgacTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGAc < 2:145665/150‑1 (MQ=255) cgacTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACgg < 1:1602634/150‑1 (MQ=255) gacTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGc > 1:1574756/1‑150 (MQ=255) cATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAgcc < 2:1244941/150‑1 (MQ=255) cATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAgcc > 1:334489/1‑150 (MQ=255) tCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAgccgg > 2:424462/1‑150 (MQ=255) aCCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAgccggcc < 2:473739/150‑1 (MQ=255) aCCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAgccggcc < 1:709782/150‑1 (MQ=255) cAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCga > 2:64444/1‑150 (MQ=255) aaGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGAt < 2:1205563/113‑1 (MQ=255) aaGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGAt > 1:1205563/1‑113 (MQ=255) ccGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGtcggc > 1:1471352‑M2/1‑85 (MQ=255) ccGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGtcggc < 2:1471352‑M2/90‑6 (MQ=255) cGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATgg < 2:131277/150‑1 (MQ=255) tcGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCa > 1:194923/1‑121 (MQ=255) tcGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCa < 2:194923/121‑1 (MQ=255) gccgcGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTAc < 2:1494291/150‑1 (MQ=255) cgcgTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGACCTCGAtcggcgcctcgct < 1:794981‑M2/88‑14 (MQ=255) cgcgTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGACCTCGAtcggcgcctcgct > 2:794981‑M2/1‑75 (MQ=255) gTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAAt < 1:927442/150‑1 (MQ=255) tGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGcc < 2:1574756/150‑1 (MQ=255) gTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCctg < 2:916320/150‑1 (MQ=255) gcgACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCctgct < 1:826133/147‑1 (MQ=255) gcgACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCctgct > 2:826133/1‑147 (MQ=255) gACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGACCTCGAtcggcgcctcgct > 2:1647608‑M2/1‑57 (MQ=255) gACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGACCTCGAtcggcgcctcgct < 1:1647608‑M2/70‑14 (MQ=255) aCTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCAAGGCGCCGATCGAGGTCGGCGGCCGGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCCTGCTGCAGtt > 2:1421865/1‑150 (MQ=255) tGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATggg < 1:158862/121‑1 (MQ=255) tGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATggg > 2:158862/1‑121 (MQ=255) aaGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCCTGCTGCAgatcggaa > 1:1250248/1‑143 (MQ=255) aGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCCTGCTGCAGTTTTTCAt < 2:334489/150‑1 (MQ=255) gTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCCTGCTGCAGTTTTTCATGAcc < 1:424462/150‑1 (MQ=255) cgcACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGGCCTACCGAATAACGCCTGCTGCAGGTTTTAATGAGCCAGCAGGAg > 2:423818/1‑150 (MQ=255) aCTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGAc < 2:1237051/54‑1 (MQ=255) aCTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGAc > 1:1237051/1‑54 (MQ=255) gAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCCTGCTGCAGTTTTTCATGACCCACCAGGAGCGCGCCTATACCCGCGGCCaa < 2:705886/150‑1 (MQ=255) gAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCCTGCTGCAGTTTTTCATGACCCACCAGGAGCGCGCCTATACCCGCGGCCAACTGCTGGATCAg < 2:1049471/150‑1 (MQ=255) | CGCCAAGGGTGAAGAGGACAACAAGATCCAGGGCCTGGAAGTGGGCGCCGACGACTACATCACCAAGCCGTTCTCGCCGCGTGAACTGGTAGCGCGACTGAAAGCCGTGCTGCGCCGCACTGGCCCGAGCGACAGCGAGGCGCCGATCGAGGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGTGTGACCATCGACGGCAAGCCGGCCGAGATGGGCCCTACCGAATACCGCCTGCTGCAGTTTTTCATGACCCACCAGGAGCGCGCCTATACCCGCGGCCAACTGCTGGATCAG > NC_002947/6065446‑6065743 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |