Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	646,991	C→A	6.4%	A204D (GCC→GAC)	acoR →	acetoin catabolism regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	646,991	0	C	A	6.4%	195.9 / 3.4	63	A204D (GCC→GAC)	acoR	acetoin catabolism regulatory protein
Reads supporting (aligned to +/- strand):  ref base C (27/32);  new base A (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

GCCGCCTTCATCGGCCTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATGGCCGCTTGCAGGCGATCAACAGC  >  NC_002947/646846‑647136                                                                                                                                                   |                                                                                                                                                  gccgccTTCATCGGCCTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCcgc                                                                                                                                               >  2:433358/1‑150 (MQ=255)     ccTTCATCGGCCTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCTGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCgaga                                                                                                                                           <  2:1180557/150‑1 (MQ=255)      cTTCATCGGCCTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAG‑ACGGCCCGCGAGAt                                                                                                                                          <  1:1065386/150‑1 (MQ=255)      cTTCATCGGCCTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGAt                                                                                                                                          >  2:447454/1‑150 (MQ=255)       ttCATCGGCCTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATc                                                                                                                                         <  1:1424449/150‑1 (MQ=255)            cGGCCTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGaaaa                                                                                                                                    >  1:395557/1‑150 (MQ=255)             ggCCTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAAc                                                                                                                                   <  1:181849/150‑1 (MQ=255)               ccTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATc                                                                                                                                         <  1:1385160/142‑1 (MQ=255)               ccTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATc                                                                                                                                         >  2:1385160/1‑142 (MQ=255)               ccTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGc                                                                                                                                 <  2:1002391/150‑1 (MQ=255)                 tGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGGAA‑ACGGCCCGCGAGATCGAAAACGCct                                                                                                                               <  1:1278298/150‑1 (MQ=255)                     cTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCcttctt                                                                                                                           <  1:24995/150‑1 (MQ=255)                        cacaGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCAt                                                                                                                        >  1:267591/1‑150 (MQ=255)                         acaGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATg                                                                                                                       <  1:433358/150‑1 (MQ=255)                          caGCGGCGCCGGTGTTCGACCGGTTGGGTGAGTTGTTGGGTGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCTAGTTGGTCGAGCAA‑ACGGCTCGCGAGATCGAAAACGCCTTCTTCATGc                                                                                                                      <  2:224929/150‑1 (MQ=255)                          caGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGc                                                                                                                      >  1:608403/1‑150 (MQ=255)                           aGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGca                                                                                                                     >  2:865679/1‑150 (MQ=255)                                   cGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGccc                                                                                                             <  1:701072/150‑1 (MQ=255)                                           gACCCGTTGGGTGAGTTGTTGGGCGTGGTGTATGTGTCGGCCTGGCAGGCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCAc                                                                                                     >  1:36151/1‑150 (MQ=255)                                            aCCCGTTGGGTGAGTTGTTGGGCGTGGTGGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGTTATTAACGGTGGGCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCATTCTTCATGGATAGTAGGCAGGGCCACt                                                                                                    >  1:515049/1‑150 (MQ=255)                                             cccGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTg                                                                                                   >  2:413868/1‑150 (MQ=255)                                                gTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGt                                                                                                <  2:395557/150‑1 (MQ=255)                                                 ttGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTg                                                                                               <  1:1422988/150‑1 (MQ=255)                                                              tgGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCGCCTGATCCGCCAGTTGGTCGAGCAA‑CCGGCCCGCGAGATCGACAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGGTGCGCGCCCATg                                                                                  <  2:822937/150‑1 (MQ=255)                                                                ggCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATg                                                                                                                       >  2:1139207/1‑111 (MQ=255)                                                                ggCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATg                                                                                                                       <  1:1139207/111‑1 (MQ=255)                                                                ggCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCAAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGt                                                                                >  2:149805/1‑150 (MQ=255)                                                                               tCGGCCTTGCAGTCGCCGGACGATCGACCCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTAtgtg                                                                 <  1:413868/150‑1 (MQ=255)                                                                                cGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGc                                                                                                                                 <  1:490049/85‑1 (MQ=255)                                                                                cGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGc                                                                                                                                 >  2:490049/1‑85 (MQ=255)                                                                                  gCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCCGGCTATGTTgat                                                              <  2:1593990/150‑2 (MQ=255)                                                                                   ccTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGcaca                                                                                                                   >  1:6506/1‑96 (MQ=255)                                                                                   ccTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGcaca                                                                                                                   <  2:6506/96‑1 (MQ=255)                                                                                   ccTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTC‑ACCAAGACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGgaga                                                             <  1:430533/150‑1 (MQ=255)                                                                                             gCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGa                                                   >  1:924147/1‑150 (MQ=255)                                                                                                    gATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGAc                                                  <  2:1379939/144‑1 (MQ=255)                                                                                                    gATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGAc                                                  >  1:1379939/1‑144 (MQ=255)                                                                                                      tCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCggg                                                                        >  2:187841/1‑120 (MQ=255)                                                                                                      tCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCggg                                                                        <  1:187841/120‑1 (MQ=255)                                                                                                      tCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACctgct                                          >  1:828732/1‑150 (MQ=255)                                                                                                               ccagcACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGa                                 >  1:1517327/1‑150 (MQ=255)                                                                                                                cagcACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGAt                                >  2:112118/1‑150 (MQ=255)                                                                                                                cagcACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGAt                                >  1:1590999/1‑150 (MQ=255)                                                                                                                 agcACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATg                               >  1:1467233/1‑150 (MQ=255)                                                                                                                    aCCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCg                            >  2:1640689/1‑150 (MQ=255)                                                                                                                      cTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATg                                                                                  <  1:1109248/94‑1 (MQ=255)                                                                                                                      cTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATg                                                                                  >  2:1109248/1‑94 (MQ=255)                                                                                                                      cTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGAt                          <  1:326100/150‑1 (MQ=255)                                                                                                                        gATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATgg                        >  1:1437400/1‑150 (MQ=255)                                                                                                                        gATCCGCCAGTTGGTCGAGCAA‑ACGGACCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACctgctg                                         <  1:635775/133‑1 (MQ=255)                                                                                                                        gATCCGCCAGTTGGTCGAGCAA‑ACGGACCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACctgctg                                         >  2:635792/1‑133 (MQ=255)                                                                                                                        gATCCGCCAGTTGGTCGAGCAA‑ACGGACCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACctgctg                                         <  1:635792/133‑1 (MQ=255)                                                                                                                        gATCCGCCAGTTGGTCGAGCAA‑ACGGACCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACctgctg                                         >  2:635775/1‑133 (MQ=255)                                                                                                                             gCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTAtgt                                                                  <  2:1506368/103‑1 (MQ=255)                                                                                                                             gCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTAcgt                                                                  >  1:1506368/1‑103 (MQ=255)                                                                                                                                   tGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATGGCCGCTTGCAgg             <  2:1590999/150‑1 (MQ=255)                                                                                                                                      tCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATGGCCGCTTGCAGGCGa          <  1:112118/150‑1 (MQ=255)                                                                                                                                        gAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATGGCCGCTTGCAGGCGATc        >  2:3423/1‑150 (MQ=255)                                                                                                                                           cAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATGGCCGCTTGCAGGCGATCAAc     <  1:865679/150‑1 (MQ=255)                                                                                                                                           cAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATGGCCGCTTGCAGGCGATCAAc     <  2:36151/150‑1 (MQ=255)                                                                                                                                           cAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATGGCCGCTTGCAGGCGATCAAc     <  2:1517327/150‑1 (MQ=255)                                                                                                                                             a‑aCGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATGGCCGCTTGCAGGCGATCAACAg   <  1:1525091/150‑1 (MQ=255)                                                                                                                                               gcGGCCCGCGAGATCGAAAACCCCTTCTTAATGCCCAGCGCCCGGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATGGCCGCTTGCAGGCGATCAACAGc  <  2:267591/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GCCGCCTTCATCGGCCTGACCTGCACAGCGGCGCCGGTGTTCGACCCGTTGGGTGAGTTGTTGGGCGTGGTAGATGTGTCGGCCTTGCAGTCGCCGGACGATCGGCGCAGCCAGCACCTGATCCGCCAGTTGGTCGAGCAA‑ACGGCCCGCGAGATCGAAAACGCCTTCTTCATGCACAGCGCCCAGGGCCACTGGGTGATGCGCGCCCATGGTACGCCGGGCTATGTGGAGAGCCAGCCCGACTACCTGCTGGCCTGGGATGCCGATGGCCGCTTGCAGGCGATCAACAGC  >  NC_002947/646846‑647136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑