Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 813,204 T→C 7.0% intergenic (+108/+23) PP_0699 → / ← PP_t70 LysE family transporter/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947813,2040TC 7.0% 167.1 / 2.7 57intergenic (+108/+23)PP_0699/PP_t70LysE family transporter/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (24/29);  new base C (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

CGGGCTTGGCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTT  >  NC_002947/813057‑813351
                                                                                                                                                   |                                                                                                                                                   
ggggCTTGGCCTGGGTGTGGCGGTGACCGGAATACGTGGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGc                                                                                                                                                   <  2:714/149‑1 (MQ=255)
          cTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCt                                                                                                                                         <  2:282800/150‑1 (MQ=255)
            ggCTGTGGCGGTGACCGGACTACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCGCAAGGTAAGCCCttt                                                                                                                                       <  2:1079679/150‑1 (MQ=255)
            ggCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCttt                                                                                                                                       <  2:694812/150‑1 (MQ=255)
                    cGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCGCAAGGTAAGCCCttt                                                                                                                                       >  2:1303026/1‑142 (MQ=255)
                    cGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCGCAAGGTAAGCCCttt                                                                                                                                       <  1:1303026/142‑1 (MQ=255)
                       tGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtgg                                                                                                                            <  1:354301/150‑1 (MQ=255)
                        gACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggt                                                                                                                           <  1:145273/150‑1 (MQ=255)
                           cGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGc                                                                                                                        >  2:615276/1‑150 (MQ=255)
                           cGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTACGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGc                                                                                                                                                   <  1:163684/123‑1 (MQ=255)
                           cGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTACGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGc                                                                                                                                                   >  2:163684/1‑123 (MQ=255)
                             gAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTa                                                                                                                      <  2:1483263/150‑1 (MQ=255)
                                   gTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTc                                                                                                                                    <  2:242356/130‑1 (MQ=255)
                                   gTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTc                                                                                                                                    >  1:242356/1‑130 (MQ=255)
                                               aTAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAAcccc                                                                                                    >  2:641303/1‑150 (MQ=255)
                                                     gcgcCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGAccc                                                                                              <  2:604239/150‑1 (MQ=255)
                                                         cGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCa                                                                                            <  1:871609/148‑1 (MQ=255)
                                                         cGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCa                                                                                            >  2:871609/1‑148 (MQ=255)
                                                          gAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCa                                                                                         >  2:1350135/1‑150 (MQ=255)
                                                            aaCCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCAtt                                                                                       <  1:615276/150‑1 (MQ=255)
                                                              ccTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTAt                                                                                     <  2:1503067/150‑1 (MQ=255)
                                                                tgtgATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAgg                                                                                                                >  1:707052/1‑121 (MQ=255)
                                                                tgtgATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAgg                                                                                                                <  2:707052/121‑1 (MQ=255)
                                                                  tgATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAat                                                                                 <  2:660687/150‑1 (MQ=255)
                                                                  tgATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAat                                                                                 <  2:1378543/150‑1 (MQ=255)
                                                                     ttCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAatgct                                                                              >  1:1094134/1‑150 (MQ=255)
                                                                            cccGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCAATATGAATGCTATGctct                                                                       <  1:641303/150‑1 (MQ=255)
                                                                                  tgctgcGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATTCTCTAACCaa                                                                 >  2:419518/1‑150 (MQ=255)
                                                                                           aTTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTAt                                                                                     >  1:1562559/1‑121 (MQ=255)
                                                                                           aTTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTAt                                                                                     <  2:1562559/121‑1 (MQ=255)
                                                                                               gttggCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACCTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAg                                                    >  2:415236/1‑150 (MQ=255)
                                                                                                    cgcgGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGAt                                               <  2:1454299/150‑1 (MQ=255)
                                                                                                               gCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCatta                                    <  2:1485941/150‑1 (MQ=255)
                                                                                                               gCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCatta                                    >  2:1369897/1‑150 (MQ=255)
                                                                                                                   cTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggtg                                                                                                                          >  2:1389329/1‑60 (MQ=255)
                                                                                                                   cTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggtg                                                                                                                          <  1:1389329/60‑1 (MQ=255)
                                                                                                                    tttttCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCa                                                                                         >  2:695123/1‑92 (MQ=255)
                                                                                                                    tttttCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCa                                                                                         <  1:695123/92‑1 (MQ=255)
                                                                                                                    tttttCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATtctc                               <  1:95196/150‑1 (MQ=255)
                                                                                                                         cGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACCTGAACCCCGGACCCCAGCATTACGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGaa                          <  1:415236/150‑1 (MQ=255)
                                                                                                                          gCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAAGGCCGCGCCGCGGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAAc                         >  2:55775/1‑150 (MQ=255)
                                                                                                                              gCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAAc                                                                    >  1:546939/1‑103 (MQ=255)
                                                                                                                              gCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAAc                                                                    <  2:546939/103‑1 (MQ=255)
                                                                                                                               cAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAgg                                                                                                                >  2:20010/1‑58 (MQ=255)
                                                                                                                               cAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAgg                                                                                                                <  1:20010/58‑1 (MQ=255)
                                                                                                                               cAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAGCCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGcccc                    >  1:122947/1‑150 (MQ=255)
                                                                                                                                caaGACAAAGGGCTTACCGCAAGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGccccc                   >  1:827864/2‑150 (MQ=255)
                                                                                                                                aaaGGAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGccccc                   >  2:1113549/1‑150 (MQ=255)
                                                                                                                                aaaGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGCCTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGccccc                   >  1:81044/1‑150 (MQ=255)
                                                                                                                                aaaGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGccccc                   >  2:324209/1‑150 (MQ=255)
                                                                                                                                           gCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCaa                                                                 <  1:356077/93‑1 (MQ=255)
                                                                                                                                           gCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCaa                                                                 >  2:356077/1‑93 (MQ=255)
                                                                                                                                            cTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTAt                                                                                     <  2:1624016/72‑1 (MQ=255)
                                                                                                                                            cTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTAt                                                                                     >  1:1624016/1‑72 (MQ=255)
                                                                                                                                            cTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTa                                                     >  2:1128697/1‑104 (MQ=255)
                                                                                                                                            cTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTa                                                     <  1:1128697/104‑1 (MQ=255)
                                                                                                                                                 cTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCAttt  <  1:1113549/150‑1 (MQ=255)
                                                                                                                                                  ttGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAg                                                            <  1:1432269/91‑1 (MQ=255)
                                                                                                                                                  ttGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAg                                                            >  2:1432269/1‑91 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGGGCTTGGCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTT  >  NC_002947/813057‑813351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑