Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 877,904 C→T 5.1% G93D (GGC→GAC)  hprA ← glycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947877,9040CT 5.1% 228.8 / 2.4 79G93D (GGC→GAC) hprAglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (34/40);  new base T (2/2);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

GCAGGCAGAACTGGCTGGCCTTGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCG  >  NC_002947/877755‑878051
                                                                                                                                                     |                                                                                                                                                   
gcaggcagAACTGGCTGGCCTTGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGTCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGc                                                                                                                                                     >  2:847856/1‑150 (MQ=255)
   ggcagAACTGGCTGGCCTTGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCt                                                                                                                                                  <  2:1145481/150‑1 (MQ=255)
              ctggcCTTGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGggcg                                                                                                                                       <  2:1280915/150‑1 (MQ=255)
               tggcCTTGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGggcgg                                                                                                                                      >  1:316592/1‑150 (MQ=255)
                ggcCTTGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGGCAACAGCAGAGCCAGGGTATGCTGGGCTACAGACGGGGTGCCGTAGCCCTGGCAGTTGCACACGGGTATGCCCTGGGCACGggcggc                                                                                                                                     >  1:163165/1‑150 (MQ=255)
                 gcCTTGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTg                                                                                                                                                 >  1:1579879/1‑137 (MQ=255)
                 gcCTTGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTg                                                                                                                                                 <  2:1579879/137‑1 (MQ=255)
                  ccTTGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCAAAAGGTAATGCCATGGGGACAggcggcgg                                                                                                                                   >  2:1169524/1‑150 (MQ=255)
                    ttGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGcc                                                                                                                                 >  1:137291/1‑150 (MQ=255)
                                 cATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACAttgt                                                                                                                    <  2:254058/150‑1 (MQ=255)
                                       ccACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGc                                                                                                              >  2:984270/1‑150 (MQ=255)
                                         aCCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCTGCGGCCAGGTCGACATTGTTGGTGCCg                                                                                                            >  1:751791/1‑150 (MQ=255)
                                          ccGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCgg                                                                                                           <  2:712477/150‑1 (MQ=255)
                                               tGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTggcg                                                                                                      <  2:316592/150‑1 (MQ=255)
                                               tGGTTGTAGGCGCACCGCCGAGTGGCCAGGGCAAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAAGTCGACATTGTTGGTGCCGGTggcg                                                                                                      <  2:283115/150‑1 (MQ=255)
                                                ggTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTggcgg                                                                                                     <  2:680148/150‑1 (MQ=255)
                                                 gTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGggc                                                                                                                                        >  2:1014630/1‑114 (MQ=255)
                                                 gTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGggc                                                                                                                                        <  1:1014630/114‑1 (MQ=255)
                                                 gTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTggcggc                                                                                                    <  2:137291/150‑1 (MQ=255)
                                                  ttGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGcc                                                                                                   >  2:1125406/1‑150 (MQ=255)
                                                      aGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGccacca                                                                                               <  2:325620/150‑1 (MQ=255)
                                                          gCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGAt                                                                                           >  1:294253/1‑150 (MQ=255)
                                                           cacaGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAACCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTACCGGGGGCGGCCACCAGGATc                                                                                          >  1:902830/1‑150 (MQ=255)
                                                              aGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTCAGTGCCGGTGGCGGCCACCAGGATCAGc                                                                                       <  1:863471/150‑1 (MQ=255)
                                                              aGCCGAGTGGCCAGGGCAAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGACCTGGTCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGc                                                                                       <  2:632252/150‑1 (MQ=255)
                                                                ccGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCtt                                                                                     >  1:1345086/1‑150 (MQ=255)
                                                                  gAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGcca                                                                                                  >  2:1415374/1‑135 (MQ=255)
                                                                  gAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGcca                                                                                                  <  1:1415374/135‑1 (MQ=255)
                                                                  gAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCa                                                                                   <  1:1125406/150‑1 (MQ=255)
                                                                        ccAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCCGTTGCACACGGTGTTGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCg                                                                             >  1:198132/1‑150 (MQ=255)
                                                                          aGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACggg                                                                                                                                         <  2:1218437/88‑1 (MQ=255)
                                                                          aGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACggg                                                                                                                                         >  1:1218437/1‑88 (MQ=255)
                                                                            ggCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGtt                                                                         >  1:1067776/1‑150 (MQ=255)
                                                                               cAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTggc                                                                      >  1:1389872/1‑150 (MQ=255)
                                                                               cAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTggc                                                                      >  1:562655/1‑150 (MQ=255)
                                                                                   agcagGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGcc                                                                  >  1:607669/1‑150 (MQ=255)
                                                                                        ggCCTGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTg                                                                        <  2:93216/139‑1 (MQ=255)
                                                                                        ggCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTg                                                                        >  1:93216/1‑139 (MQ=255)
                                                                                               gTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTgcggtg                                                      >  1:303388/1‑148 (MQ=255)
                                                                                               gTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTgcggcg                                                      >  1:1123366/1‑150 (MQ=255)
                                                                                                tATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGt                                                     <  2:294253/150‑1 (MQ=255)
                                                                                                 aTGCTGGGCTACAGACGGCGTGCCGTAGCCGTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCTAGGTTGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTc                                                    <  2:129709/150‑1 (MQ=255)
                                                                                                    cTGGGCTACAGACGGCTGGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCGTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAg                                                 <  2:198132/150‑1 (MQ=255)
                                                                                                    cTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAg                                                 <  1:533511/150‑1 (MQ=255)
                                                                                                    cTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCGGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAg                                                 <  2:467226/150‑1 (MQ=255)
                                                                                                     tGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTgg                                                                                                                                                >  1:681573/1‑54 (MQ=255)
                                                                                                     tGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTgg                                                                                                                                                <  2:681573/54‑1 (MQ=255)
                                                                                                       ggCGACATACGGCGTGCCGTAGGCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCAt                                              <  2:562655/150‑1 (MQ=255)
                                                                                                        gCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATg                                             >  1:240540/1‑150 (MQ=255)
                                                                                                           aCAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGAcc                                          <  1:1169524/150‑1 (MQ=255)
                                                                                                            cAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTg                                                                        <  1:251716/119‑1 (MQ=255)
                                                                                                            cAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTg                                                                        >  2:251716/1‑119 (MQ=255)
                                                                                                             aGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCtt                                        >  1:729146/1‑150 (MQ=255)
                                                                                                              gACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCttg                                       <  1:1447554/150‑1 (MQ=255)
                                                                                                                 ggCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCttgttg                                    <  2:647445/150‑1 (MQ=255)
                                                                                                                    gTGCCGTAGCCCTGGCAGTTGCACACGGTGATGTCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCgg                                                                            >  1:337177/1‑107 (MQ=255)
                                                                                                                    gTGCCGTAGCCCTGGCAGTTGCACACGGTGATGTCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCgg                                                                            <  2:337366/107‑1 (MQ=255)
                                                                                                                    gTGCCGTAGCCCTGGCAGTTGCACACGGTGATGTCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCgg                                                                            <  2:337177/107‑1 (MQ=255)
                                                                                                                    gTGCCGTAGCCCTGGCAGTTGCACACGGTGATGTCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCgg                                                                            >  1:337366/1‑107 (MQ=255)
                                                                                                                      gCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGACCGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGAc                               <  1:950242/150‑1 (MQ=255)
                                                                                                                       ccGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGAcc                              >  1:973527/1‑150 (MQ=255)
                                                                                                                       ccGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGAcc                              <  2:1067776/150‑1 (MQ=255)
                                                                                                                            gCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGc                         <  1:489233/150‑1 (MQ=255)
                                                                                                                             cccTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGcc                        >  2:850336/1‑150 (MQ=255)
                                                                                                                              ccTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCa                       >  1:221914/1‑150 (MQ=255)
                                                                                                                              ccTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCa                       >  1:888965/1‑150 (MQ=255)
                                                                                                                              ccTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCa                       >  1:1560716/1‑150 (MQ=255)
                                                                                                                               cTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCAc                      >  2:203336/1‑150 (MQ=255)
                                                                                                                                  gaaGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGgc                   <  2:240540/148‑1 (MQ=255)
                                                                                                                                  gCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGTGTCGAGCATGACCTTGTTGCTGACCACTGCCACGgc                   <  2:303388/150‑1 (MQ=255)
                                                                                                                                  gCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGgc                   <  2:1123366/150‑1 (MQ=255)
                                                                                                                                       tGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCt              <  2:811325/150‑1 (MQ=255)
                                                                                                                                          acacGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCa           >  2:1629997/1‑150 (MQ=255)
                                                                                                                                          acacGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCa           >  1:464198/1‑150 (MQ=255)
                                                                                                                                           cacGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCaa          <  2:464198/150‑1 (MQ=255)
                                                                                                                                             cGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGc        <  2:1345086/150‑1 (MQ=255)
                                                                                                                                                 gATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTc    <  1:984270/150‑1 (MQ=255)
                                                                                                                                                  aTGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTcc   <  2:729146/150‑1 (MQ=255)
                                                                                                                                                  aTGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTcc   <  2:973527/150‑1 (MQ=255)
                                                                                                                                                   tGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCcg  <  1:76413/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCAGGCAGAACTGGCTGGCCTTGGCCCACTGGCCATCGGCCACCGCCTGGTTGTAGTCGCACAGCCGAGTGGCCAGGGCCAACAGCAGGGCCAGGGTATGCTGGGCTACAGACGGCGTGCCGTAGCCCTGGCAGTTGCACACGGTGATGCCCTGGGCACGGGCGGCGGCCAGGTCGACATTGTTGGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCG  >  NC_002947/877755‑878051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑