Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 944,212 T→C 8.1% A471A (GCA→GCG)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947944,2120TC 8.1% 140.4 / 3.2 49A471A (GCA→GCG) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (26/19);  new base C (2/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

TACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGC  >  NC_002947/944067‑944346
                                                                                                                                                 |                                                                                                                                      
tACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCgg                                                                                                                                    <  1:1080169/150‑1 (MQ=35)
      tGAGGGTAGTGCCGTCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTgggg                                                                                                                              <  2:441850/150‑1 (MQ=255)
       gAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGc                                                                                                                             >  2:245015/1‑150 (MQ=35)
              gtgCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCg                                                                                                                      >  1:1419143/1‑150 (MQ=39)
                       gCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCCGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAg                                                                                                             >  2:1095033/1‑150 (MQ=255)
                               ggCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCCGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGtt                                                                                                     <  1:1095033/150‑1 (MQ=255)
                                   aGATTGCTCGGGCTGTCGGGCGCGGTGGCCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCa                                                                                                 >  1:299684/1‑150 (MQ=255)
                                     aTTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCAcc                                                                                               <  1:777755/150‑1 (MQ=38)
                                        gCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGc                                                                                                                                      >  1:1073901/1‑108 (MQ=38)
                                        gCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGc                                                                                                                                      <  2:1073901/108‑1 (MQ=38)
                                        gCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCAc                                                                                            <  1:1644859/150‑1 (MQ=38)
                                          tCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGa                                                                                          >  1:1062615/1‑150 (MQ=39)
                                                      gcgcGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGa                                                                              <  1:245015/150‑1 (MQ=38)
                                                               tCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGa                                                                                          >  2:1428415/1‑129 (MQ=40)
                                                               tCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGa                                                                                          <  1:1428415/129‑1 (MQ=40)
                                                                          tCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGtt                                                                                                     >  2:899195/1‑107 (MQ=40)
                                                                          tCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGtt                                                                                                     <  1:899195/107‑1 (MQ=40)
                                                                          tCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATcg                                                          >  1:327828/1‑150 (MQ=255)
                                                                                    tAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGa                                                <  1:879158/150‑1 (MQ=255)
                                                                                       ccACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGa                                                                                                                           <  1:1131601/72‑1 (MQ=255)
                                                                                       ccACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGa                                                                                                                           >  2:1131601/1‑72 (MQ=255)
                                                                                       ccACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAg                                             >  1:1389537/1‑150 (MQ=255)
                                                                                                 aTTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTgg                                                                                                                                >  2:244420/1‑57 (MQ=35)
                                                                                                 aTTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTgg                                                                                                                                <  1:244420/57‑1 (MQ=38)
                                                                                                  ttgccgctTGGGTCGGTCTGCACCAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgt                                    >  1:874587‑M2/4‑109 (MQ=255)
                                                                                                  ttGCCGCTTGGGTCGGTCTGCACCAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgt                                    <  2:874587‑M2/148‑40 (MQ=255)
                                                                                                   tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGt                                                                                   <  1:1086409/100‑1 (MQ=35)
                                                                                                   tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGt                                                                                   >  2:1086409/1‑100 (MQ=37)
                                                                                                   tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCt                                 <  2:216510/150‑1 (MQ=255)
                                                                                                   tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCt                                 >  1:216510/1‑150 (MQ=255)
                                                                                                              tcggtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCg                      >  1:870195/1‑150 (MQ=255)
                                                                                                                 gtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGt                                                                                                      <  1:23709/67‑1 (MQ=37)
                                                                                                                 gtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGt                                                                                                      >  2:23709/1‑67 (MQ=37)
                                                                                                                 gtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTc                   >  1:1063046/1‑150 (MQ=255)
                                                                                                                  tcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa                  >  1:1377240/1‑150 (MQ=255)
                                                                                                                  tcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa                  >  2:346195/1‑150 (MQ=255)
                                                                                                                  tcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa                  >  2:394768/1‑150 (MQ=255)
                                                                                                                         cAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgtgatcggcacgggtgttgtg                 <  2:1359512‑M2/144‑59 (MQ=255)
                                                                                                                         cAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgtgatcggcacgggtgttgtg                 >  1:1359512‑M2/1‑86 (MQ=255)
                                                                                                                         cAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAg           >  2:159098/1‑150 (MQ=255)
                                                                                                                         cAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAg           <  2:299684/150‑1 (MQ=255)
                                                                                                                         cAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAg           <  2:1063046/150‑1 (MQ=255)
                                                                                                                            gCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGcc        <  2:1389537/150‑1 (MQ=255)
                                                                                                                              tCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGt      >  1:68086/1‑150 (MQ=255)
                                                                                                                                aaCTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCa    >  1:719139/1‑150 (MQ=255)
                                                                                                                                aaCTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCa    >  2:333680/1‑150 (MQ=255)
                                                                                                                                  cTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGc  >  1:296110/1‑150 (MQ=255)
                                                                                                                                             gCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa                  <  2:1375037/123‑1 (MQ=255)
                                                                                                                                             gCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa                  >  1:1375037/1‑123 (MQ=255)
                                                                                                                                                 |                                                                                                                                      
TACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGC  >  NC_002947/944067‑944346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑