Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 155,543 G→T 8.4% intergenic (‑248/+35) yjbB ← / ← ygdQ Na+/Pi‑cotransporter/transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947155,5430GT 8.4% 180.6 / 3.9 58intergenic (‑248/+35)yjbB/ygdQNa+/Pi‑cotransporter/transport protein
Reads supporting (aligned to +/- strand):  ref base G (28/25);  new base T (2/3);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

TGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAG  >  NC_002947/155399‑155689
                                                                                                                                                |                                                                                                                                                  
tGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAGACGCACAGAAAAAAGGAATTAACCAGGGCCCGGCGAAg                                                                                                                                               >  2:261313/1‑150 (MQ=255)
 gTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGACTGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAgg                                                                                                                                              >  1:418628/1‑150 (MQ=255)
    gCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGcc                                                                                                                                           >  1:1239316/1‑150 (MQ=255)
    gCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCAAAGTGAAAAAAAAAAAAAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGcc                                                                                                                                           <  2:143433/150‑1 (MQ=255)
     ccAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGccc                                                                                                                                          >  1:1145367/1‑150 (MQ=255)
     ccAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGccc                                                                                                                                          <  2:940820/150‑1 (MQ=255)
     ccAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGccc                                                                                                                                          >  2:239712/1‑150 (MQ=255)
         cTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGTCGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTTCGCAGGGCCCtttt                                                                                                                                      <  1:1103199/150‑3 (MQ=255)
           tCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCttgttg                                                                                                                                    <  1:614928/150‑1 (MQ=255)
               aTGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGtgct                                                                                                                                <  1:1527648/150‑1 (MQ=255)
                  gCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAg                                                                                                                                               >  1:1405083/1‑132 (MQ=255)
                  gCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAg                                                                                                                                               <  2:1405083/132‑1 (MQ=255)
                      tCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTTCGCAGGGCCCtttttaat                                                                                                                                  >  1:1480275/1‑135 (MQ=255)
                      tCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTTCGCAGGGCCCtttttaat                                                                                                                                  <  2:1480275/141‑7 (MQ=255)
                        atttatttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCt                                                                                                                       >  2:810506/1‑150 (MQ=255)
                          ttatttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTg                                                                                                                     <  1:582855/150‑1 (MQ=255)
                             tttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGcc                                                                                                                  >  1:844509/1‑150 (MQ=255)
                               tGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCtt                                                                                                                <  1:1466494/150‑1 (MQ=255)
                                gAAACAGATCTTGAAACGAATGACTGCGCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTa                                                                                                               <  1:261313/150‑1 (MQ=255)
                                              aaCGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTa                                                                                                               >  2:260126/1‑136 (MQ=255)
                                              aaCGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTa                                                                                                               <  1:260126/136‑1 (MQ=255)
                                                 gAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCt                                                                                              >  1:1609422/1‑150 (MQ=255)
                                                        tGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTc                                                                                                                         >  2:1471144/1‑116 (MQ=255)
                                                        tGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTc                                                                                                                         <  1:1471144/116‑1 (MQ=255)
                                                                ggggCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGt                                                                                                 <  2:285328/132‑1 (MQ=255)
                                                                ggggCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGt                                                                                                 >  1:285328/1‑132 (MQ=255)
                                                                    ctgctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCCCAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCACTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTc                                                                           >  1:1266350/1‑150 (MQ=255)
                                                                                 aaGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCa                                                              >  2:610225/1‑150 (MQ=255)
                                                                                   gCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATc                                                            >  2:531699/1‑150 (MQ=255)
                                                                                    ccaccaCAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATcc                                                           >  1:892410/1‑150 (MQ=255)
                                                                                        cacaGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGc                                                       >  1:823723/1‑150 (MQ=255)
                                                                                          caGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCgg                                                     <  2:1459985/150‑1 (MQ=255)
                                                                                          caGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCgg                                                     <  1:239712/150‑1 (MQ=255)
                                                                                                          gAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGt                                                                                      >  2:655476/1‑101 (MQ=255)
                                                                                                          gAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGt                                                                                      <  1:655476/101‑1 (MQ=255)
                                                                                                            aaaaaCGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGa                                                                       <  1:1580192/114‑1 (MQ=255)
                                                                                                            aaaaaCGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGa                                                                       >  2:1580192/1‑114 (MQ=255)
                                                                                                                   cacaGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGt                            <  2:823723/150‑1 (MQ=255)
                                                                                                                    acaGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGtt                           >  1:1292874/1‑150 (MQ=255)
                                                                                                                     caGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTGCTTGCGAAGTTTACCCGCTTCATGACTCTAGCCGCCCGTACGGGCCTTGGCGGTGCGCGTTCGGATGTTg                          >  2:835694/1‑150 (MQ=255)
                                                                                                                     caGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTgc                                        <  1:815870/136‑1 (MQ=255)
                                                                                                                     caGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGACGGTgc                                        >  2:815870/1‑136 (MQ=255)
                                                                                                                       gAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGAt                        <  1:531699/150‑1 (MQ=255)
                                                                                                                        aaaaaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGt                                                                             <  2:825928/96‑1 (MQ=255)
                                                                                                                        aaaaaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGt                                                                             >  1:825928/1‑96 (MQ=255)
                                                                                                                           aaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCt                    <  2:1292874/150‑1 (MQ=255)
                                                                                                                             aGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTcc                                                                                               >  1:127385/1‑73 (MQ=255)
                                                                                                                             aGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTcc                                                                                               <  2:127385/73‑1 (MQ=255)
                                                                                                                                 cacaaaaaGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATcc                                                           >  1:819857/4‑105 (MQ=255)
                                                                                                                                 cacaaaaaGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATcc                                                           <  2:819857/102‑1 (MQ=255)
                                                                                                                                    aacaaGGGCCCTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGAt                        <  1:305923/134‑1 (MQ=255)
                                                                                                                                    aacaaGGGCCCTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGAt                        >  2:305923/4‑137 (MQ=255)
                                                                                                                                     aaaaGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCa          <  1:123559/150‑1 (MQ=255)
                                                                                                                                           gCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCgaga    >  1:78765/1‑150 (MQ=255)
                                                                                                                                            cccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGaa   >  1:386219/1‑150 (MQ=255)
                                                                                                                                            cccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGaa   >  2:524863/1‑150 (MQ=255)
                                                                                                                                            cccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGaa   <  2:1609422/150‑1 (MQ=255)
                                                                                                                                             ccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAg  <  1:810506/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAG  >  NC_002947/155399‑155689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑