| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,487,103 | C→T | 6.4% | intergenic (+112/+18) | yhdZ → / ← degS | amino acid ABC transporter ATP‑binding protein/serine endoprotease DegS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,487,103 | 0 | C | T | 6.4% | 183.2 / 2.6 | 62 | intergenic (+112/+18) | yhdZ/degS | amino acid ABC transporter ATP‑binding protein/serine endoprotease DegS |
| Reads supporting (aligned to +/- strand): ref base C (33/25); new base T (2/2); total (35/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
GGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCCGCGCTACCT > NC_002947/1486957‑1487251 | ggACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCa > 1:1374277/1‑150 (MQ=255) gACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCaa > 1:555542/1‑150 (MQ=255) ccTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTggg < 2:1374277/150‑1 (MQ=255) cTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGt > 2:1126833/1‑150 (MQ=255) tCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTctct < 1:1648132/150‑1 (MQ=255) cATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCctg > 2:516772/1‑150 (MQ=255) aTGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTctct < 2:817018/140‑1 (MQ=255) aTGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTctct > 1:817018/1‑140 (MQ=255) aTGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCctggctgg > 2:1550565/1‑150 (MQ=255) tGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCtggctggc < 2:1103698/150‑1 (MQ=255) gcgGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTgg > 2:1305964/1‑150 (MQ=255) CCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCctgg < 2:175112‑M1/122‑1 (MQ=255) CCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCctgg > 1:175112‑M1/1‑122 (MQ=255) TTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGc > 1:1509070‑M1/1‑150 (MQ=255) TCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTCATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGcc > 2:1345399‑M1/1‑150 (MQ=255) CGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGTAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCATGGCTGCCAGCCGGCGCCGGGGGCGGCCGCAgccccc < 2:1441593‑M1/150‑2 (MQ=255) GGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCAcc < 1:145415‑M1/150‑1 (MQ=255) ACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCtcc < 1:842213‑M1/150‑2 (MQ=255) cccgctcccacaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCg > 2:1608262‑M2/75‑150 (MQ=255) CCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCg < 1:1072308‑M1/150‑1 (MQ=255) CCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGa < 1:1440363‑M1/150‑1 (MQ=255) CCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGa > 1:1168691‑M1/1‑150 (MQ=255) GCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATc > 2:555542‑M1/1‑150 (MQ=255) tcccacaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAg > 2:1616409‑M2/70‑150 (MQ=255) ccacaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTAt > 1:386667‑M2/68‑89 (MQ=255) ccacaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTAt < 2:386667‑M2/22‑1 (MQ=255) CCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCt < 1:421562‑M1/150‑1 (MQ=255) cacaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcTGCCGCGCAGCCCTGGGTTTATTTCTCTTc < 1:330056‑M2/30‑1 (MQ=255) cacaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcTGCCGCGCAGCCCTGGGTTTATTTCTCTTc > 2:330056‑M2/67‑96 (MQ=255) CACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCa > 1:105383‑M1/1‑128 (MQ=255) CACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCa < 2:105383‑M1/128‑1 (MQ=255) acaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcTGCAAcgc > 1:764892‑M2/66‑73 (MQ=255) acaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcTGCAAcgc < 2:764892‑M2/8‑1 (MQ=255) acaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTtct > 1:821805‑M2/66‑91 (MQ=255) acaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTtct < 2:821805‑M2/26‑1 (MQ=255) acaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTc > 1:679035‑M2/66‑150 (MQ=255) acaggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTc > 2:473630‑M2/66‑150 (MQ=255) caggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCctggctg < 2:1608198‑M2/37‑1 (MQ=255) caggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCctggctg > 1:1608198‑M2/65‑101 (MQ=255) aggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCctggctg > 2:354308‑M2/64‑100 (MQ=255) aggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCctggctg < 1:354308‑M2/37‑1 (MQ=255) aggaattacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCaa > 1:954692‑M2/64‑150 (MQ=255) AGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCaa > 1:1305964‑M1/1‑150 (MQ=255) attacgcaggctccaaagccagcgctaaacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAActgc > 2:1260452‑M2/60‑150 (MQ=255) gcgcTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACtgctgc > 1:825985/1‑150 (MQ=255) cgcTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCctggctg > 1:1000896/1‑92 (MQ=255) cgcTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCctggctg < 2:1000896/92‑1 (MQ=255) gcTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGccc > 1:169778/1‑150 (MQ=255) ggCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCa < 2:1415746/149‑1 (MQ=255) tAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATg < 2:825985/150‑1 (MQ=255) ttCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGtt > 1:151593/1‑150 (MQ=255) aacctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGtt < 1:473630‑M2/118‑1 (MQ=255) ctgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTAtc < 2:145415‑M2/121‑1 (MQ=255) tgtgggagcgggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTAtct > 1:1260519‑M2/29‑150 (MQ=255) gggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTgg < 1:1126833‑M2/131‑1 (MQ=255) ggcgtgcccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTggg < 1:1641902‑M2/132‑1 (MQ=255) ccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTtctc > 2:48968‑M2/12‑137 (MQ=255) ccgcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTtctc < 1:48968‑M2/126‑1 (MQ=255) gcgaagggcCGCAACGCGGCCCTGGGTTTATTTCTCTGCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCcg > 1:1364873‑M2/10‑150 (MQ=255) cgaagggcCGCAACGCGGCCCTGGGTTTCTTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCcgc < 2:1440363‑M2/142‑1 (MQ=255) agggcCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTGGTTGGGCTTGATCCGCGCt > 2:1079741‑M2/6‑150 (MQ=255) cCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCCGCGCTACCt > 1:246769‑M2/2‑150 (MQ=255) | GGACCAAGTTGTTCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCCGCGCTACCT > NC_002947/1486957‑1487251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |