Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,549,937 A→T 6.5% I99F (ATC→TTC)  groL → chaperonin GroEL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,549,9370AT 6.5% 233.2 / 5.3 75I99F (ATC→TTC) groLchaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base A (37/32);  new base T (2/3);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGT  >  NC_002947/1549790‑1550086
                                                                                                                                                   |                                                                                                                                                     
aCCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATc                                                                                                                                                     >  2:1229860/1‑150 (MQ=255)
aCCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATc                                                                                                                                                     >  2:398807/1‑150 (MQ=255)
 ccACGGACGGCGTTTCCGTGGCCACAGAAATCGAGCAGACAGCCGCGCGCGCAAACATGGGCTCCCAGCTGGTCAAGGAAGTTGCTTGCAACGCCAACGACGCTGCCGGTGACGCCACCACCGCCGCTACCGTTCTGCCTCAGGCCATCg                                                                                                                                                    >  2:443517/1‑150 (MQ=255)
     ggACGGCGTTTGAGTCGCAAAAGAAATTGAGGTGTAAGACTCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCaa                                                                                                                                                <  2:353025/150‑1 (MQ=255)
      gACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAAc                                                                                                                                               <  2:1068890/150‑1 (MQ=255)
         ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGCCTAGCGACGCGCTACTTCACCCTACCACTACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGaa                                                                                                                                            <  1:445096/150‑1 (MQ=255)
          gCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAATGTGCTTAAACGATGCACGACATTTCAGTGGGCTGCACCACCACCGCTACCGTTCTGGCTCAGGCCATAGTCGACGAAg                                                                                                                                           >  1:1427268/1‑150 (MQ=255)
          gCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAg                                                                                                                                           <  1:398807/150‑1 (MQ=255)
             tttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGcc                                                                                                                                        >  1:491000/1‑150 (MQ=255)
                ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGa                                                                                                                                     <  2:95232/150‑1 (MQ=255)
                  gTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGaaa                                                                                                                                   <  2:491000/150‑1 (MQ=255)
                   tCGCCAAGGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAg                                                                                                                                  <  1:271842/150‑1 (MQ=255)
                      ccAAAGAAATCGAGCTGAAAGATGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCg                                                                                                                               >  2:1624107/1‑150 (MQ=255)
                              agcgacttGAAAGACGCTTTCCAAAACATGGGCGCCCACCTGGTCAAGGAAGTTTCTTCCAAGGCCAACGACGCTGCTGGGGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCTGTCGCTGcc                                                                                                                       <  2:537226/143‑1 (MQ=255)
                               tCGAGCTGAAAGACGCTTTCGACAACATGGGCTCCCAGCTGGTCAAGGAAGTTGATTGCAAGGCCAACCACGCTGCCGGTGACGGAACCACAACCGCTACCGTTCTGGCTAACGCCACCGTAACCGAAGGTCTGAAAGCCGTGGCTGCCg                                                                                                                      >  2:764331/1‑150 (MQ=255)
                                 gAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGc                                                                                                                    >  2:1544201/1‑150 (MQ=255)
                                           aCGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGa                                                                                                          >  2:503081/1‑150 (MQ=255)
                                           aCGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGa                                                                                                          >  2:1614384/1‑150 (MQ=255)
                                            ctcTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGCCCTCACACCCGTAACAAATCCCGTTCCTGGCGCGGTTAACGAATTCCTGAAAGCCGTCGCTGCCGGCATGAACCCGAt                                                                                                         <  1:444956/148‑1 (MQ=255)
                                               tttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATgag                                                                                                      >  2:444956/1‑148 (MQ=255)
                                                ttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGAc                                                                                                     >  1:612546/1‑150 (MQ=255)
                                                  cGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCt                                                                                                   <  1:1614384/150‑1 (MQ=255)
                                                   gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGGTTCCAAGGCCAACGACGCTGCCGGTGACGGAACCATCACCGCTAACGTTCTTGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTACCGGCATGAACCTGATGGACCTg                                                                                                  >  2:464499/1‑150 (MQ=255)
                                                   gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGATGGCCTGa                                                                                                                                     <  2:941598/115‑1 (MQ=255)
                                                   gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGATGGCCTGa                                                                                                                                     >  1:941598/1‑115 (MQ=255)
                                                         aTGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACAGTTCTGGCTCAGGACAGTGTCAATCAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcgc                                                                                            <  1:199940/150‑1 (MQ=255)
                                                              cgcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGCCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTAt                                                                                       <  2:1041827/150‑1 (MQ=255)
                                                               gcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc                                                                                      <  1:148083/150‑1 (MQ=255)
                                                                        gTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGcc                                                                             >  1:1476956/1‑150 (MQ=255)
                                                                           aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGTACCCGATGGACCTGAAGCGCGGTATCGACAAg                                                                                >  1:1320376/1‑144 (MQ=255)
                                                                           aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACaa                                                                                 >  2:1073491/1‑143 (MQ=255)
                                                                           aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACaa                                                                                 <  1:1073491/143‑1 (MQ=255)
                                                                           aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAg                                                                                <  2:1320376/144‑1 (MQ=255)
                                                                              gAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActgct                                                                       >  1:1596148/1‑150 (MQ=255)
                                                                               aaGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActgctg                                                                      <  2:1427268/150‑1 (MQ=255)
                                                                               aaGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActgct                                                                       >  1:828463/1‑149 (MQ=255)
                                                                               aaGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActgct                                                                       <  2:828463/149‑1 (MQ=255)
                                                                                aGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACtgctgc                                                                     >  1:441797/1‑150 (MQ=255)
                                                                                  ttGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcg                                                                   >  1:1493556/1‑150 (MQ=255)
                                                                                          aaGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAACGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGAAAAAGCGACTACTGCcgtggtgatc                                                           >  2:506193/1‑146 (MQ=255)
                                                                                            ggCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                                                                           >  1:269401/1‑116 (MQ=255)
                                                                                            ggCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                                                                           <  2:269401/116‑1 (MQ=255)
                                                                                            ggCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGa                                                         >  2:1483647/1‑150 (MQ=255)
                                                                                             gCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGaa                                                        >  1:12619/1‑150 (MQ=255)
                                                                                             gCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGaa                                                        <  2:373205/150‑1 (MQ=255)
                                                                                              ccAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAAc                                                       <  2:1069688/150‑1 (MQ=255)
                                                                                                aaCGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTg                                                     >  1:167154/1‑150 (MQ=255)
                                                                                                aaCGACGCTGCCGGTGACGGCACCACCACCCCTACCCTTACGACATCGACCATCGCGAATTAAGGCCTGTGAGTTGACGCTGCCGGAATAAACCCCATGGACCTGAGGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTg                                                     >  1:29659/1‑150 (MQ=255)
                                                                                                      gCTGCCGGTGCCGGCACCACCTGCTCTAGCATTCTGACGCAGGCCTCCGTTAAAGACGGGCGGTAAGCCTACCCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAAc                                               <  1:443517/150‑1 (MQ=255)
                                                                                                      gCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAAc                                               <  1:1624107/150‑1 (MQ=255)
                                                                                                      gCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAAc                                               <  2:612546/150‑1 (MQ=255)
                                                                                                           cGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTc                                          <  2:167154/150‑1 (MQ=255)
                                                                                                              tGACCGCACCACGACCGCTACCGTTCTGGCTCAGGCCATCGTAAACGAAGGCCAGTAGGTAGTCGCAGGCTTCATGAAACCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCaa                                       <  1:529818/150‑1 (MQ=255)
                                                                                                                  ggCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGt                                                                                         >  2:429493/1‑96 (MQ=255)
                                                                                                                  ggCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGt                                                                                         <  1:429493/96‑1 (MQ=255)
                                                                                                                  ggCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATTGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCa                                   >  1:1255586/1‑150 (MQ=255)
                                                                                                                       caccaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                                                                           <  2:1293367/89‑1 (MQ=255)
                                                                                                                       caccaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                                                                           >  1:1293367/1‑89 (MQ=255)
                                                                                                                       caccaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATgcgc                              <  2:1476956/150‑1 (MQ=255)
                                                                                                                        accaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGcc                             >  2:700958/1‑150 (MQ=255)
                                                                                                                        accaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGcc                             <  2:778878/150‑1 (MQ=255)
                                                                                                                        accaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGcc                             >  1:778878/1‑150 (MQ=255)
                                                                                                                        accaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGcc                             >  1:695170/1‑150 (MQ=255)
                                                                                                                         ccaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCg                            >  1:1636268/1‑150 (MQ=255)
                                                                                                                              gCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc                                                                                      >  2:1502760/1‑87 (MQ=255)
                                                                                                                              gCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc                                                                                      <  1:1502760/87‑1 (MQ=255)
                                                                                                                                tACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCAACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCaa                     <  1:294844/150‑1 (MQ=255)
                                                                                                                                        tGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCg             >  1:1473720/1‑150 (MQ=255)
                                                                                                                                          tttCAGGCCTTCGTTGACGATGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACt                                                      >  1:1392854/3‑107 (MQ=38)
                                                                                                                                          tttCAGGCCTTCGTTGACGATGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACt                                                      <  2:1392854/105‑1 (MQ=38)
                                                                                                                                           cTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGccc          <  1:1544201/150‑1 (MQ=255)
                                                                                                                                            tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa         <  2:1596148/150‑1 (MQ=255)
                                                                                                                                              aGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCagg       >  1:1083722/1‑150 (MQ=255)
                                                                                                                                                  cATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCag        >  1:840470/1‑145 (MQ=255)
                                                                                                                                                  cATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCag        <  2:840470/145‑1 (MQ=255)
                                                                                                                                                   aTCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtaggt  <  1:503081/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGT  >  NC_002947/1549790‑1550086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑